Hepatitis C Virus-Related Lymphomagenesis in a Mouse Model
Figure 1
(A) The structure of the hepatitis C virus (HCV) transgene (HCR6-Rz); HCV gene expression was regulated by the Cre/loxP expression cassette (upper). The Cre transgene was located in the CD19 locus (bottom). (B) Expression of HCV core protein in the liver, spleen, and plasma of RzCD19Cre mice was quantified by core ELISA. Data represent the mean (SD) (). (C) Histological analysis of tissues from a normal mouse (a, c, e; lymph node from CD19Cre mouse; b, d, f) and B cell lymphoma (b, d, f; RzCD19Cre mouse). Paraformaldehyde-fixed and paraffin-embedded tumor tissues were stained with hematoxylin and eosin (H&E) (c–f); immunostaining of lymphoma with anti-CD45R (g) and anti-CD3 (h) is indicated. Scale bars, 100 μm (c, d) and 20 μm (g, h); arrow heads indicate mitotic cells. (D) Concentration of sIL-2Rα in serum samples from tumor-free control mice, and RzCD19Cre and wild-type (WT) mice with or without B-cell lymphomas or other tumors.
