Single Layer Centrifugation Can Be Scaled-Up Further to Process up to 150 mL Semen
Figure 3
SLC-150. Notes: (a) After centrifugation, the different layers can be seen, comprising 150 mL seminal plasma and extender on top of 150 mL colloid, with the sperm pellet in the bottom of the tube; (b) Removing the supernatant after centrifugation using a water pump; (c) Aspirating the sperm pellet.
