Single Layer Centrifugation Can Be Scaled-Up Further to Process up to 150 mL Semen

Table 1

Summary of the volumes of colloid and extended semen*, centrifuge tubes, and centrifuges used in these experiments (modified from [14]).

Species	Tube size	Volume of colloid (mL)	Volume of extended semen* (mL)	Name of technique	Centrifuge
Stallion/boar	12 mL	4.0	4.5	SLC-4.5	1
Stallion/boar	50 mL	15	15–18	SLC-15	1
Stallion/boar	100 mL	20	25	SLC-25	2
Stallion/boar	200 mL	60	60	SLC-60	3
Boar**	500 mL	150	150	SLC-150	1

^*Extended to give a concentration of approximately 100 × 10⁶ spermatozoa/mL.
^**this technique has not been attempted yet with stallion semen but is theoretically possible.
Centrifuge 1: for example: Hereaus Multifuge, Kendro, Osterode, Germany;
Centrifuge 2: for example: Sigma Laboratory Centrifuge, Osterode, Germany;
Centrifuge 3: for example: Centra MP 4R, International Equipment Company, MA, USA.