248280.fig.005a
(a)
248280.fig.005b
(b)
Figure 5: (a) Phorbol ester treatment does not alter global protein expression. Zebrafish larvae were treated with (1) DMSO, (2) 1 μM prostratin, (3) 10 μM prostratin, and (4) 10 μM PMA. Equivalent concentrations of protein samples were subjected to PAGE (b) Phorbol esters upregulate MAPK-8 in treated larvae. Zebrafish larvae were treated with (1) DMSO, (2) 1 μM prostratin, (3) 10 μM prostratin, and (4) 10 μM PMA. Equivalent concentrations of larval lysates were subjected to PAGE/Western analysis using zebrafish specific MAPK-8 antisera.