Helicobacter pylori Induces Disturbances in Gastric Mucosal Akt Activation through Inducible Nitric Oxide Synthase-Dependent S-Nitrosylation: Effect of Ghrelin

Figure 6

Effect of Akt kinase inhibitor, SH-5 (SH), on ghrelin-induced cNOS phosphorylation in the gastric mucosal cells exposed to H. pylori LPS. The cells were treated with ghrelin (Gh) at 0.5 $\mu$g/ml or Akt inhibitor, SH-5 (30 $\mu$M) + Gh, and incubated for 16 h in the presence of 100 ng/ml LPS. Cell lysates were resolved on SDS-PAGE, transferred to nitrocellulose, and probed with phosphorylation-specific cNOS (pcNOS) antibody, and after stripping reprobed with anti-cNOS antibody. The immunoblots shown are representative of three experiments.