Helicobacter pylori Induces Disturbances in Gastric Mucosal Akt Activation through Inducible Nitric Oxide Synthase-Dependent S-Nitrosylation: Effect of Ghrelin

Figure 8

Effect of iNOS inhibitor, 1400W, cNOS inhibitor, L-NAME, and ascorbate on the ghrelin-(Gh-) induced changes in Akt kinase threonine (Thr³⁰⁸) and serine (Ser⁴⁷³) phosphorylation in gastric mucosal cell exposed to H. pylori LPS. The cells, preincubated with 30 $\mu$M 1400W (14 W), 300 $\mu$M L-NAME (LN), or 300 $\mu$M ascorbate (As), were treated with Gh at 0.5 $\mu$g/ml and incubated for 16 h in the presence of 100 ng/ml LPS. Values represent the means $\pm$ SD of five experiments. ${}^{*}P<.05$ compared with that of control. ${}^{**}P<.05$ compared with that of LPS alone. ${}^{***}P<.05$ compared with that of Gh + LPS.