Helicobacter pylori Induces Disturbances in Gastric Mucosal Akt Activation through Inducible Nitric Oxide Synthase-Dependent S-Nitrosylation: Effect of Ghrelin

Figure 9

Effect of ghrelin (Gh) on H. pylori LPS-induced Akt S-nitrosylation. The gastric mucosal cells were treated with iNOS inhibitor, 1400W (30 $\mu$M), or Gh (0.5 $\mu$g/ml) and incubated for 16 h in the presence of 100 ng/ml LPS. A portion of the cell lysates were processed by biotin switch procedure for protein S-nitrosylation, along with the reminder of the lysates, resolved on SDS-PAGE, transferred to nitrocellulose and probed with phospho-Akt (Ser⁴⁷³) antibody, and after stripping reprobed with anti-Akt antibody. The immunoblots shown are representative of three experiments.