Genetic Structure of Sympatric Sexually and Parthenogenetically Reproducing Population of Chara canescens (Charophyta)

Table 2

Characterization of four microsatellite loci developed for Chara canescens; 𝑛=604. 𝑇_𝑎 is the annealing temperature used in the analysis and 𝑁_𝐴 the is number of alleles determined.

Locus	Repeat motif	Primer sequences (5′–3′)	𝑇a (°C)	𝑁A	Size range (bp)	Accession no.
ChcanA9	(CA)n	F: GAGCATCCGCAGTAATCC	55	14	147–213	FN430830
		R: CCTCTCTGCACGTCTGTG
ChcanA19	(GT)n (GA)n	F: GCTTACTTCGCGCATCTAG	60	17	127–175	FN430831
		R: GACGAGTGCCATCTGATTC
ChcanA6	(CA)n	F: AAACTGGATCGGAGGTACTG	60	16	171–237	AM710335
		R: GCAGAGACTGTTCTTGACATG
ChcanA1	(AC)n	F: CACTTTGCTGCTTGCTCCAT	60	16	191–267	FN430832
		R: AGGAGATCAGACAGTAAGCC