Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models
Figure 6
Impaired growth and survival of fission yeast expressing E46K α-synuclein. (a) Survival assay schematic: 1000 yeast cells expressing WT or E46K α-synuclein or vector alone were first grown in inducing media (without thiamine for fission yeast and galactose for budding yeast) for 8 hours (early log phase) or 18 hours (late log phase) and then plated onto repressing (+thiamine for fission yeast and glucose for budding yeast) or inducing (−thiamine or galactose). Images of plates were taken either two or five days of growth and yeast colonies were examined for size and number . (b) Composite images of portions of representative plates that contain TCP1 cells grown in repressed conditions for two days or induced conditions for two or five days. (c and d) Same as B, but for SP3 and BY4741 cells, respectively. After two or five total days of continuous expression, E46K α-synuclein cells remain significantly smaller than WT in SP3 cells, but not in TCP1 cells or BY4741 cells.
