Feasibility of Repurposing the Polyanionic Microbicide, PPCM, for Prophylaxis against HIV Transmission during ART
Figure 3
Lack of effect of PPCM on sperm motility, forward progression and viability. Washed spermatozoa were exposed to PPCM at different concentrations and durations of exposure, as indicated. The experimental protocol for these experiments is presented in Section 2. (a): Neither concentration nor duration of treatment with PPCM affects sperm motility. Values are the means ยฑ SEM of duplicate determinations from each of 3 donors (number of donors represents sample size). Two-way ANOVA shows no effect of either duration of treatment (; ) or dose of PPCM (; ) after exposure to 10โmg/mL PPCM for up to 90โmin. Similarly, one-way ANOVA shows no overall treatment effect of PPCM (; ). (b): Neither concentration nor duration of exposure affects sperm forward progression. Values are the means ยฑ SEM of duplicate determinations from each of 3 donors (number of donors represents sample size). Two-way ANOVA shows no effect of either duration of treatment (; ), or concentration of PPCM (; ). Similarly, one-way ANOVA shows no effect of treatment (; ). (c): Duration of exposure to, but not dose of PPCM has small effect on sperm viability. Data are expressed as percentage of postwash control viability, as described in Section 2. Values are the means ยฑ SEM of duplicate determinations from each of 3 donors (number of donors represents sample size). Two-way ANOVA shows an effect of duration of treatment (; ), but not concentration of PPCM (; ); no interactive effect of dose and exposure time is seen. The Newman-Keuls multiple range test indicates essentially no effect among treatment groups (). One-way ANOVA shows an overall effect of treatment (; ). Viability at 90โmin for samples exposed to either 5โmg/mL or 10โmg/mL PPCM is reduced by 11% as compared with the untreated sample at 90โmin (, Newman-Keuls multiple range test).
