Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model

Figure 2

Antimicrobial effects of diode laser on E. faecalis in test tubes. The bacteria were exposed to laser irradiation with or without an added photosensitizer (closed diamond, without photosensitizer; closed square, with photosensitizer) for 30, 60, and 120 seconds. (A) Elimination of E. faecalis in model of apical periodontitis. E. faecalis in the presence and absence of a photosensitizer[PS(−), without photosensitizer; PS(+), with photosensitizer] in the lesion defect area of the model were exposed to diode laser irradiation for 30, 60, and 120 seconds. In addition, one sample was irrigated with sterilized saline for 120 seconds as a negative control (saline-120 s) and another with 2.5% NaOCl for 120 seconds as a positive control (2.5% NaOCl-120 s). (B) The number of viable cells after each treatment was counted. Data are expressed as the mean ± standard deviation of triplicate determinations. The experiment was performed three times, with similar results obtained in each.