Figure 4: In vitro effect of FBPase and GS antisense cDNAs expressed in human glioma-derived cell line. Invasiveness (a) was determined in semisolid medium by measuring the number of foci that each cell can induce. Doubling time (b) of various cells is determined using counting of viable cells. Value represents the mean of 5 experiments (corresponding to 3 sets of transfection) ± SEM. < 0.05, < 0.01, < 0.001 versus empty plasmid, and statistical analysis was made using Student test. Data adapted from Blin et al. .