Lipid Droplet Binding of Hepatitis C Virus Core Protein Genotype 3
Figure 2
Colocalization of GFP-Core fusion constructs with ADRP in transfected cells. (a) Cells expressing GFP-Core fusion constructs were analyzed using immunofluorescence with antibodies to ADRP. Separate green channel (GFP) and red channel (ADRP) images are presented as well as a merged image (Merge) to illustrate colocalization as defined by yellow overlap of the green and red fluorescence. A portion of each merged image (white box) was magnified (Mag) to provide a more detailed view of fluorescent overlap. DAPI was used as a nuclear counterstain. (b) Cells expressing untagged HCV Core domain 1-2 construct from genotype 1 (G1 d1-2) and steatosis-associated genotype 3 (G3 d1-2). Cells were analyzed using HCV Core monoclonal antibody (α-Core, green channel), ADRP antibody (red channel) and a merged image (yellow overlap). DAPI was used as a nuclear counterstain.
