Detection and Typing of Human Papilloma Virus by Multiplex PCR with Type-Specific Primers

Table 1

Multiplex PCR primer list.

Name	Forward primer sequence	Reverse primer sequence	lane	size bp	R.E.	Digested fragment sizes
6-1	acgtggccttgtgcggtacagtc	agagacgagtcaggcaatgc	4	757	HaeIII	259, 250, 101, 72, 68, 7
6-2	tgtcccatctgcgcaccgaagac	cgtacactgtttgtgggcgcttc	4	592	AluI	366, 129, 97
11-1	agttccgtagatgccaagggca	tgcctcaggtgaggcccaatgc	3	528	RsaI	194, 172, 94, 68
11-2	tggtaccccctacacagggtgg	acagaatgttggacagggtcagg	3	730	HaeIII	433, 160, 137
16-1	ttaggcagcacttggccaacca	taatccgtcctttgtgtgagct	3	207	MspI	110, 97
16-2	actgcaatgtttcaggacccac	cgaagcgtagagtcacacttgc	2	661	MspI	405, 199, 57
18-1	tcgcgtcctttatcacagggcga	tgcccaggtacaggagactgtg	1	536	AluI	235, 200, 101
18-2	tccgtggtgtgcatcccagcag	cacttgtgcatcattgtggacc	2	274	RsaI	185, 48, 41
26-1	tggtatacaacgagtgtcagctcc	ggggcaatgatggccatgtcg	7	635	MspI	425, 210
31-1	aggcacggttggtgaatcggtc	tagatgctgagggtgcactacg	1	683	HaeIII	481, 202
31-2	catgaactaagctcggcattgg	tccaacatgctatgcaacgtcc	2	385	RsaI	233, 152
33-1	agcttagaggtgtggctttgtg	tgcagttagttgcagtacgtgc	2	493	RsaI	211, 145, 122, 15
33-2	tgacccacctacagctgcaatc	gggtgtgtacattatccacatcg	2	899	RsaI	615, 277, 7
35-1	ccaccaagtggttccaacgcag	tgtaggcgtgtagctgtgtagc	3	488	RsaI	216, 193, 79
35-2	gtcctgttggaaaccaacacgt	acacacagacgtagtgtcgcct	3	251	AluI	135, 81, 35
39-1	acacaaacggtgtattccgtgcca	tgtgcagttggagatttgggatcc	5	200	RsaI	129, 71
39-2	tgtgcagtaccagtgacggatcg	atttttggcgttgtgactctgtg	6	438	HaeIII	221, 217
45-1	ggacatcacacctaccgtggac	ctgtgaggtggacacacggacc	4	298	RsaI	126, 69, 59, 26, 18
45-2	acctgcacaattgcaacctggt	caactgccaggggtttcacgca	4	345	RsaI	179, 105, 61
51-1	aattgctggcaacgtacacgac	acacttgaacacctgcaacacg	4	255	RsaI	190, 51, 14
51-2	cctactccaggggttagtcgca	taaggagggcaactgcctagac	4	504	HaeIII	270, 234
52-1	cccaagtgtaacgtcatgcgtg	agggttgtttatagccgtgcac	3	323	AluI	215, 108
52-2	acctccgcagtgtccgtgggtg	aagagcggcctaagcactgcac	1	601	RsaI	143, 126, 96, 95, 66, 41, 34
53-1	ttgttcagtgtacggggctagc	gtgacgccattgcagttatcgcct	6	549	MspI	389, 160
53-2	ttctgcagtaagctatgagggcat	aaccactgtcgatttcggtgtt	7	449	MspI	270, 179
56-1	ctgggcactaggtcaaagcctgct	caaccacgcgtaaaagcactcat	6	307	AluI	278, 29
58-1	ggtagtaccccaccgtctgagg	agacgtgacattgccactgtca	1	414	MspI	289, 125
58-2	accagactccagagacaacacc	tcacctttgtcatcactggtcc	1	264	RsaI	165, 62, 25, 12
59-1	agacaccgttacatgagctgct	tcattctcggagtcggagtcag	1	320	AluI	213, 91, 16
59-2	tctaacgccatctgcagcaagg	acagtagtccactgacacgctg	4	438	HaeIII	339, 99
66-1	tgcggtagtatccttgggcagtg	tacaataagggctacacgccaa	5	388	RsaI	135, 131, 122
68-1	ggtactgcttggaacacgcctg	ggcccccagacatagggacctt	6	368	RsaI	307, 57, 4
68-2	gtcaaaaagacgcccctgcaccta	cacaccttagggtagggctacaa	5	490	HaeIII	329, 161
73-1	acaggctattagttgccaacgtc	ttcttaggtgtggcacttgtg	6	222	AluI	115, 62, 45
73-2	ggggtgggcaaaggtaggtagc	acaatccaggggcctctggtccga	7	322	RsaI	189, 108, 25
82-1	tgtccgtggacacctgcgacca	gtagttaaaggtgatgtggcaacc	7	546	RsaI	275, 215, 56
82-2	cccaaaaccaatacacgtgctgaa	aacatcctgttggtcgttgcca	5	270	HaeIII	189, 81
β-globin	gaagagccaaggacaggtac	caacttcatccacgttcacc	7	268