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ISRN Nanotechnology
Volume 2012 (2012), Article ID 187354, 9 pages
http://dx.doi.org/10.5402/2012/187354
Research Article

Encapsulation of Berberine in Nano-Sized PLGA Synthesized by Emulsification Method

1Department of Chemistry, Momin College, Maharashtra, Bhiwandi, India
2N.S.N. Research Centre for Nanotechnology & Bionanotechnology, Jambhul Phata, Maharashtra, Ambernath 421 505, India

Received 30 April 2012; Accepted 17 July 2012

Academic Editors: Y. I. Jeong, C. Li, and P. Perriat

Copyright © 2012 Manisha Khemani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nanoparticles of PLGA (polylactide glycolic acid) were prepared using biodegradable poly (D, L-lactide-co-glycolide)—75 : 25, by emulsification method using PVA (Mol. Wt. 9000) or didodecyl dimethyl ammonium bromide (DMAB) as surfactant. Nanoparticles were morphologically characterized using scanning electron microscope (SEM) and particle size analyzer. The distribution of size of PLGA nanoparticles was in the range of 48–211 nm. Berberine, a yellow isoquinoline alkaloid that is used as traditional anticancer drug, was loaded on to PLGA nanoparticles by single emulsion as well as multiple emulsion solvent evaporation techniques. Particle size analysis showed an increase in berberine loaded PLGA NP size to 180–310 nm when PVA was used as a stabilizer. Whereas use of DMAB as a stabilizer led to precipitation. In vitro drug release analysis revealed that acidic pH of 5.5 was more suitable for release of berberine than pH 7.4.