Nitric Oxide Synthase Gene Transfer Overcomes the Inhibition of Wound Healing by Sulfur Mustard in a Human Keratinocyte In Vitro Model
Figure 1
Effect of AIVP transfection on iNOS protein expression after scratching with or without SM treatment: NHEK cells were infected immediately after scratching either with AIVP or ANVP (mock) at a 1 : 5,000 dilution of the original stock shown in Table 1 immediately after scratching with or without SM treatment, and cells were then collected 24 h after scratching and subjected to Western blot analysis (a). The total cellular protein loaded in each lane was 10 μg. The same PVDF membrane was reprobed with β-actin antibody to use as a loading control in Western blot analysis (b). The image was a representative of 3 independent Western blot experiments. Lanes: 1: control (ANVP transfection, no scratch, no transfection, no SM); 2: AIVP transfection (no scratch); 3: scratch only; 4: scratch with AIVP; 5: SM only (no scratch, ANVP); 6: scratch with SM; 7: scratch (SM, AIVP); M: molecular markers.
