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ISRN Organic Chemistry
Volume 2012 (2012), Article ID 208284, 8 pages
Synthesis of Macrocyclic Bis-Hydrazone and Their Use in Metal Cations Extraction
Department of Chemistry, Faculty of Science, University of Damascus, Damascus, Syria
Received 9 October 2011; Accepted 30 October 2011
Academic Editor: G. Li
Copyright © 2012 Farouk Kandil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Two new macrocyclic hydrazone Schiff bases were synthesized by reaction of succindihydrazide and adipdihydrazide with acetylacetone. Hydrazones have been characterized by elemental analyses and IR, mass, 1H NMR, and 13C NMR spectral data. Hydrazones have been studied by liquid-liquid extraction towards the s-metal ions (Li+, Na+, and K+) and d-metal ions (Cu2+ and Cr3+) from aqueous phase to organic phase. The effect of chloroform and dichloromethane as organic solvents over the metal chlorides extraction was investigated at 25 ± 0.1°C by using flame atomic absorption. We found differences between the two solvents in extraction selectivity.
Hydrazones are special class of compounds in the Schiff bases family. They are characterized by the presence of the following (1) Hydrazone Schiff bases of acyl, aroyl, and heteroaroyl compounds have an addition donor sites like C=O. The additional donor sites make them more flexible and versatile. This versatility has made hydrazones good polydentate chelating agents that can form a variety of complexes with various transition and inner transition metals and have attracted the attention of many researchers .
A wide varieties of hydrazones and their metal complexes have been studied because of their important properties which can be used in different applications, such as, extraction of some metal ions , microdetermination of metal ions , determination of titanium in bauxite, Portland cement, amphibolites granites , and different biological activities, such as antimicrobial [2, 4–7], antifungal [8, 9], antitumor [10, 11], and insecticides . For these applications, we are extending this field of compounds for synthesising novel macrocyclic hydrazones.
Five dissymmetric tridentate Schiff base ligands containing a mixed donor set of ONN and ONO were prepared by the reaction of benzohydrazide with the appropriate salicylaldehyde and pyridine-2-carbaldehyde and characterized by FT-IR, 1H NMR, and 13C NMR .
Inoue et al.  reported the synthesis and spectroscopic characterization of complexes of Ni2+, Cu2+, Zn2+, and Cd2+ with hydrazones Schiff bases derived from 6-amino-5-formyl-1,3-dimethyluracil, nicotinic and isonicotinic acids hydrazides. Avaji et al.  reported the synthesis and characterization of a macrocyclic hydrazone Schiff bases prepared by condensation of 1,4-dicarbonyl phenyl dihydrazide with 2,6-diformyl-4-methyl phenol. Emara et al. reported [16–18] formation of binuclear complexes for the ligands derived from 4,6-diacetylresorcinol, where the ligands were prepared by condensation of 4,6-diacetylresorcinol (DAR) with oxalyldihydrazine (ODH) in the molar ratios (1 : 1) and (1 : 2) to afford the corresponding hydrazone, H6La and H4Lb, ligands, respectively. Otomo and Nakayama et al.  reported formation of three terdentate hydrazones, all containing the 1-phthalazino grouping in the hydrazine moiety but differing in the heterocyclic substituent in the aldehyde moiety, where the ligands were used as analytical reagents for palladium(II), the optimal conditions for the extractive spectrophotometric determination of palladium(II) in the presence of chloride ions being deduced. These compounds were highly selective and sensitive reagents for palladium(II), since they were not extracted into chloroform from sulfuric acid solutions and did not react with other platinum group metals. Odashima and Ishii  reported the synthesis and spectroscopic characterization of four new hydrazones, 2-pyridinecarbaldehyde 3-nitro-2-pyridylhydrazone, 2-pyridinecarbaldehyde 3,5-dinitro-2-pyridylhydrazone (PA-3,5-NPH), 2-quinolinecarbaldehyde 5-nitro-2-pyridylhydrazone, and 6-phenanthridinecarbaldehyde 5-nitro-2-pyridylhydrazone. Also useful information on the molecular design of the hydrazone reagent was obtained. A highly sensitive and practical extraction-spectrophotometric method for the determination of nickel with PA-3,5-NPH developed and applied successfully to the determination of nickel in steel samples.
In the context of the above applications, we have reported here the synthesis and characterization of novel macrocyclic hydrazone Schiff bases and their extraction study of some transition metal cations. The structure of Schiff bases and scheme synthesis is shown in Scheme 1.
2.1. Reagents and Apparatus
All the chemicals used were of AnalaR grade and procured from Sigma-Aldrich and Fluka. Metal salts were purchased from E. Merck and were used as received. Distilled water was used in extraction experimentals. The solvents were saturated with each other before use in order to prevent volume changes of the phases during extraction.
The C, H, and N were analyzed on a Carlo-Erba 1106 elemental analyzer. The IR spectra was recorded on Jusco 300 instrument in KBr pellets. 1H and 13C-NMR spectra of ligands in CDCl3 solution were recorded on a Bruker DT-400 MHz spectrometer, and chemical shifts are indicated in ppm relative to tetramethylsilane. Mass spectra were recorded using a KRATOS MS50TC spectrometer.
AA 929 Unicam Spectrometer was used for FAAS measurements with an air-acetylene flame. A pH meter (Metrohm 691 pH Meter) was also used. All extractions experiments were performed by using a mechanical flask agitator in 50 cm3 stoppered glass flasks.
2.2. Synthesis of Diethyl Succinate
A succinic acid (9.19 mmol) in absolute dry ethanol (60 mL) containing 2-3 drops of concentrated H2SO4 was refluxed, continuing the heating for 20–30 minutes. When the reflux time is complete, remove the flask from the heating mantle, allow it to cool briefly. Then, the reaction mixture was poured onto ice cold water. Place about 5 mL chipped ice in a 50-mL beaker and mix it with 15 mL 10% aqueous Na2CO3 (sodium carbonate) (the pH should be above 7. If it is not, add more (10%) Na2CO3 dropwise until the solution is basic). The ester thus obtained was extracted by diethyl ether. Then dry the ether solution in the centrifuge tube by adding several spatula-tip loads of anhydrous Na2SO4 .
2.3. Synthesis of Diethyl Adipate
Adipic acid (7.92 mmol) in absolute dry ethanol (60 mL) containing 2-3 drops of concentrated H2SO4 was refluxed, then continue the heating for 20–30 minutes. When the reflux time is complete, remove the flask from the heating mantle, allow it to cool briefly. Then, the reaction mixture was poured onto ice cold water. Place about 5 mL chipped ice in a 50-mL beaker and mix it with 15 mL 10% aqueous Na2CO3 (sodium carbonate)(the pH should be above 7. If it is not, add more 10% Na2CO3 dropwise until the solution is basic). The ester thus obtained was extracted by diethyl ether. Then dry the ether solution in the centrifuge tube by adding several spatula-tip loads of anhydrous Na2SO4 .
2.4. Synthesis of Succinic Acid Dihydrazide
Mixture of diethyl ester of succinic acid (2.22 g) and hydrazine hydrate (98% 2 cc) in ethanol (80 mL) was refluxed for 4-5 h. The reaction mixture was allowed to cool in room temperature, then the cooled solution was poured onto ice cold water. The dihydrazide of Succinic acid thus obtained was filtered and recrystallized from ethanol.
2.5. Synthesis of Dihydrazide of Adipic Acid Dihydrazide
A mixture of diethyl ester of adipic acid (2.22 g) and hydrazine hydrate (98% 2 cc) in ethanol (80 mL) was refluxed for 4-5 h. The reaction mixture was allowed to cool in room temperature, then the cooled solution was poured onto ice cold water. The dihydrazide of adipic acid obtained was filtered and recrystallized from ethanol.
2.6. Synthesis of Macrocyclic Succinic Acid Hydrazone Ligand(I)
The hot ethanolic solution (20 mL), of acetylacetone (2 mmol, 0.2 g), and a hot ethanolic solution (20 mL), of dihydrazide of succinic acid (2 mmol, 0.292 g), were mixed slowly with constant stirring. This mixture was refluxed at ~75°C for 9 h in presence of few drops of concentrated hydrochloric acid (pH~3). The reaction mixture was allowed to cool in room temperature, then the cooled solution was poured onto ice cold water. On cooling, white-colored precipitate is separated out, filtered, washed with cold EtOH, and dried under vacuum over P2O5 yield 50%. Elemental analysis found % (found atomic mass 420 amu), C 51.14 H 5.64; N 26.53 for C18H28N8O4.
2.7. Synthesis of Macrocyclic Adipic Acid Hydrazone Ligand(II)
The hot ethanolic solution (20 mL), of acetylacetone (2 mmol, 0.2 g), and a hot ethanolic solution(20 mL), of dihydrazide of adipic acid (2 mmol, 0.348 g), were mixed slowly with constant stirring. This mixture was refluxed at ~75°C for 9 h in presence of concentrated hydrochloric acid (pH~3). The reaction mixture was allowed to cool in room temperature, then the cooled solution was poured onto ice cold water. On cooling, white-colored precipitate is separated out, filtered, washed with cold EtOH, and dried under vacuum. Yield 55%. Elemental analysis found % (found atomic mass 420 amu), C 55.69 H 6.66; N 22.62 for C22H36N8O4.
2.8. Extraction Procedure
Aqueous solutions containing 1.5 × 10−3 mol L−1 metal chloride in appropriate buffer were equilibrated with equal volumes of the chloroform and dichloromethane solutions of the ligand 4 × 10−4 M by shaking in a mechanical shaker at 25°C. Optimum equilibration time was determined for this system. In most cases, distribution equilibrium was attained in less than 180 min and a shaking time of 120 min. The ionic strength of the aqueous phase was 0.1 M KCl in all experiments except those in which the effect of ionic strength was studied. After agitation, the solutions were allowed to stand for 120 min. The copper(II) and chrome(III) concentrations of the aqueous phase were determined by FAAS and that of the organic phase from the difference by considering the mass balance. The pH of aqueous phase was recorded as equilibrium pH.
3. Results and Discussion
The preparation of two ligands containing nitrogen and oxygen donor atoms are shown in Scheme 1. The structures of new compounds were characterized by a combination of IR, MS, 1H NMR, and 13C NMR spectral data. Compounds were synthesized according to reported procedures .
The spectrum showed a strong bands at 1715 and 1587 cm−1 in the spectrum of the Schiff base assigned to (C=O) of carbonyl and azomethine (C=N) vibrations, respectively. An intense band at 3111 cm−1 is due to the –NH-vibrations of the hydrazine group, a broad medium intense band was at 2930 cm−1 due to methylene groups, and the band at 1068 cm−1 is assigned to hydrazinic (N–N) of the ligand [21–25].
In the electron impact spectrum (Figure 1) of the ligand, we confirm the probable formula by showing a peak at 420 amu, corresponding to macrocyclic moiety [(C18H28N8O4), calculated atomic mass 420]. The series of peaks in the range, that is, 37, 40, 41, 42, 44, 55, 95, 96, 97, 101, 121, 132, 150, 179, 201 amu, and so forth, may be assigned to various fragments. Their intensity gives an idea of stability of fragments (Figure 1).
In the 1H NMR spectrum the ligand(I) exhibits signals at 2.24 ppm due to CH3–C(6H), 2.54 due to –CH2(4H), 3.55 due to –CH2–CO–(8H) and at 5.9 ppm due to NH protons (Figure 3(a)).
In the 13C NMR spectrum (Figure 3(b)) of ligand(I), and indicated new resonances are 13.80, 14.58(–CH3), 23.74, 34.90(CH2), 110.96, 143.99(CH2CO), 151.77(C=N), 173.71(–CO–NH–).
The spectrum showed strong bands at 1717 and 1581 cm−1 in the spectrum of the Schiff base assigned to (C=O) of carbonyl and azomethine (C=N) vibrations, respectively. A broad medium intense bands were at 2959 and 2873 cm−1 due to methyl groups and band at 1136 cm−1 is assigned to hydrazinic (N–N) of the Schiff base [21–25].
In the electron impact spectrum (Figure 2) of the ligand(II), we confirm the probable formula by showing a peak at 476 amu, corresponding to macrocyclic moiety [(C22H36N8O4), calculated atomic mass 476]. The series of peaks in the range, that is, 38, 39, 40, 41, 42, 43, 54, 55, 56, 96, 97, 111, 121, 138, 150, 165, 179, 201, 205, 251, 301, 302 amu, and so forth, may be assigned to various fragments. Their intensity gives an idea of stability of fragments (Figure 2).
In the 1H NMR spectrum, ligand(II) exhibits signals at 2.24 due to CH3–C(12H), 3.17 ppm due to –CO–CH2(4H), 1.8 ppm due to –CO–CH2–CH2(8H), 2.54 ppm due to –CH2–CN–(4H), and 5.9 ppm due to NH protons signal (Figure 4(a)). All these observations support the infrared conclusions.
In the 13C NMR spectrum Figure 4(b) of ligand, indicated new resonances are 13.82, 14.18, 14.45, 28.58, 29.88, 30.47, 60.66(CH2), 111.04, 144.08(CH2CO), 152.07(N=C), and 172.60(–CO–NH–).
3.1. Extraction of Metal Ions with Schiff Bases
3.1.1. Effect of pH and Solvents on the Extraction of Cu(II) and Cr(III)
To the best of our knowledge, the stability of a transition metal complex with a polydentate chelate ligand in organic phase depends on a range of factors including: number and type of the donor atoms present, the number and size of the chelate rings formed on complexation . In addition, the stability and selectivity of complexations strongly depend on the donor ability, dielectric constant of the solvent, and shape and size of the solvent molecules .
In this research, Liquid-liquid extraction experiments were performed to examine the efficiency and selectivity of hydrazones (I) and (II) in transferring s-metal ions (Li+, Na+, and K+) and d-metal ions (Cu2+ and Cr3+) from aqueous phase into chloroform. The results show that the alkali metals ions are not extracted by hydrazones (%).
Also the results (Figures 5(a) and 5(b)) show the extractability of Cu2+ and Cr3+ from the aqueous phase into organic phase by macrocyclic hydrazones (I) and (II). It is clear that the extractability results of hydrazones are different for both of the organic solvents. When dichloromethane was used as organic solvent, macrocyclic Schiff bases extracted Cu2+ ion 98% and Cr3+ 94%. It is interesting that the percentage of the extraction of the metal ions with both hydrazones (I) and (II) is high. These results suggest that N donor (the presence of the lone electron pair on the nitrogen atom in hydrazone molecules provides their basic properties) increases the percentage of the extraction of the metal ions.
omethane and chloroform are 9.1 and 4.8, respectively. Dichloromethane having a high dielectric constants is favored for the extraction of all the metal ions; there are similar results in the literature . On the other hand, the better solvation of the complexes by dichloromethane may be a valuable reason for better extraction.
From the extraction data shown in the figures, it is clear that the ligands which have N8O4 donor sets show that both of the cationcavity size and the type of binding sites in the ring contribute to the ability of Cu2+ ions and Cr3+ ions binding. As shown in the figures, the copper and chrome extraction is high within the pH range of 5.2–6, 5–7(Cu), 7-8, 7–7.5(Cr), respectively. Moreover, transition metals were extracted in the order Cu > Cr, which are in the same order of decreasing ionic radius.
3.1.2. Composition of the Extracted Species
If only mononuclear species are extracted, under the condition in which chloride does not take part in the distribution equilibrium, the extraction process may be represented by equation where (I) represents the extractant reagent and subscripts and denote the aqueous and organic phases, respectively. The extraction constant of the species CuL is given by
When CuL is the only extractable species and the metal is present in the aqueous phase predominantly as the cation Cu2+, the metal distribution ratio () and the extraction constant are related by
The effect of pH on the extraction of Cu2+ and Cr3+ ions from KCl media of ionic strength () has been studied, the logarithm of the values obtained was plotted against the corresponding pH values (according to (4) a plot of against pH at constant 4 × 10−4 M of ). A straight line with a slope of about 2 was obtained at of Cu2+ and Cr3, as shown Figures 6(a) and 6(b). The values represent the number of hydrogen ions released during the formation of metal-ligand complex and intercept .
Also Figure 7 shows the evolution of when increasing the concentration of ligands at constant chloride metal concentration with two different organic solvents. As seen from the plots, there is a linear relationship between and log[L]org, and the slope should be equal to the number of ligand molecules per cation in the extracted species. The slopes of lines are equal to 0.5 of dichloromethane and chloroform. Therefore, ligands form a 1 : 2 () complex with Cu2+ for both solvents.
3.1.3. Effect of Ionic Strength of Aqueous Phase
The influence of KCl in the concentration range of 0.1–1.0 M on the extraction efficiency of Cu2+ was studied in solutions containing 1.5 × 10−3 M Cu2+ and Cr3+ with 4 × 10−4 M ligand in organic phase. The extraction efficiency decreases with increase in ionic strength of the aqueous medium. Taking into account (5), the extraction constant () at zero ionic strength for this reaction can be correlated with the ionic strength(I) by According to the Debye-Huckel limiting law given
The activity coefficient () decreases with increase in ionic strength. At the constant pH, the activity coefficient of Cu2+ decreases as the ionic strength increase, hence decreases.
The new synthesized compounds act as tetra dentate Schiff bases. In most cases, these symmetrical compounds were obtained with yield more than 50% in some cases. Two imines (Schiff bases) were synthesized. We think that these compounds were prepared for the first time. This is confirmed by a precise review of the scientific background concerning this category of compounds. Their structures were identified by spectroscopy methods. The results indicate that H2L(I, II) in organic phase extracts efficiently Cu2+ and Cr3+ in aqueous phase containing 0.1 mol L−1 KCl in the pH range of approximately 5–7 and 7-8, respectively, at 25°C and 2 h stirring.
The authors are grateful to the Department of Chemistry, Faculty of Science, University of Damascus, Damascus, Syria for supporting of this paper.
- J. P. Cornelissen, J. H. Van Diemen, L. R. Groeneveld, J. G. Haasnoot, A. L. Spek, and J. Reedijk, “Synthesis and properties of isostructural transition-metal (copper, nickel, cobalt, and iron) compounds with 7,,8,-tetracyanoquinodimethanide(1-) in an unusual monodentate coordination mode. Crystal structure of bis(3,5-bis(pyridin-2-yl)-4-amino-1,2,4-triazole)bis(7,,8,- tetracyanoquinodimethanido)copper(II),” Inorganic Chemistry, vol. 31, no. 2, pp. 198–202, 1992.
- N. Nawar, M. A. Khattab, and N. M. Hosny, “Some metal(II) complexes of o-aminoacetophenone benzoylhydrazone (AABH): their preparation, characterization and antimicrobial activity,” Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry, vol. 29, no. 8, pp. 1365–1384, 1999.
- M.-D. Gui, R.-H. Zhang, H. Yan, H.-M. Wang, X.-B. Chen, and S.-J. Zhu, “Synthesis and magnetic and catalytic properties of manganous isonicotinoyl hydrazone complexes,” Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry, vol. 28, no. 8, pp. 1381–1391, 1998.
- Z. H. Chohan and A. Rauf, “Some biologically active mixed ligand complexes of Co(II), Cu(II) and Ni(II) with ONO, NNO and SNO donor nicotinoylhydrazine-derived ligands,” Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry, vol. 26, no. 4, pp. 591–604, 1996.
- S. K. Agarwal, J. Jain, and S. Chand, “Synthetic and antimicrobial studies of hexacoordinated ternary complexes of Mn(II) and Cu(II),” Asian Journal of Chemistry, vol. 14, no. 1, pp. 489–492, 2002.
- X. Zhu, C. Wang, W. Dong et al., “Synthesis, structure, and biological activities of the O,O′-diethylphosphorohydrazonothionate ligand and complexes with nickel(II), copper(II), and zinc(II),” Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry, vol. 32, no. 3, pp. 475–487, 2002.
- K. A. El-Manakhly, H. A. Bayoumi, M. M. Ezz Eldin, and H. A. Hammad, “Physicochemical and antimicrobial studies of some metal complexes,” Journal of the Indian Chemical Society, vol. 76, no. 2, pp. 63–64, 1999.
- K. P. Deepa, K. K. Aravindakshan, and F. Suhara, “Synthesis, characterization and antifungal studies of metal, complexes of benzoyl- and salicylylhydrazones of N-methylacetoacetanilide,” Asian Journal of Chemistry, vol. 13, no. 2, pp. 513–523, 2001.
- A. K. Panda, D. C. Dash, P. Mishra, and H. Mohanty, “Metallo ligands: part V—homo and bimetallic trinuclear complexes of copper(II), nickel(II) and cobalt(II) with 1,8-di-(2′-hydroxy-phenyl)-4,5-diphenyl-2,3,6,7-tetrazaoctan-1,3,5,7- tetraene,” Indian Journal of Chemistry, vol. 35, no. 10, pp. 848–851, 1996.
- M. F. Iskander, L. El-Sayed, N. M. H. Salem, W. Haase, H. J. Linder, and S. Foro, “Synthesis, characterization and magnetochemical studies of some copper(II) complexes derived from n-salicylidene-n-alkanoylhydrazins: X-Ray crystal and molecular structure of bis [monochloro-(μ-n-salicylidenemyristoylhydrazine) ono(-1)] dicopper(II),” Polyhedron, vol. 23, no. 1, pp. 23–31, 2004.
- C. Li, L. Wang, X. Meng, and H. Zhao, “Synthesis, characterization and antitumor activity of benzaldehyde nitrogen mustard picolinoyl hydrazone complexes,” Transition Metal Chemistry, vol. 24, no. 2, pp. 206–209, 1999.
- G. P. Pokhariyal, B. Lal, and V. K. Rastogi, “Synthesis, spectral and thermal studies of some divalent metal complexes with 8-acetyl-7-hydroxy-4-methyl coumarrin oxime-hdrazone,” Asian Journal of Chemistry, vol. 9, no. 3, pp. 541–543, 1997.
- F. Bouyagui Tamboura, P. Marcel Haba, M. Gaye, A. Salam Sall, A. Hamady Barry, and T. Jouini, “Structural studies of bis-(2,6-diacetylpyridine-bis-(phenylhydrazone)) and X-ray structure of its Y(III), Pr(III), Sm(III) and Er(III) complex,” Polyhedron, vol. 23, no. 7, pp. 1191–1197, 2004.
- Y. Inoue, T. Kanbara, and T. Yamamoto, “Preparation of a new receptor for anions, macrocyclic polythiolactam—structure and high anion-binding ability,” Tetrahedron Letters, vol. 44, no. 28, pp. 5167–5169, 2003.
- P. G. Avaji, C. H. Vinod Kumar, S. A. Patil, K. N. Shivananda, and C. Nagaraju, “Synthesis, spectral characterization, in-vitro microbiological evaluation and cytotoxic activities of novel macrocyclic bis hydrazone,” European Journal of Medicinal Chemistry, vol. 44, no. 9, pp. 3552–3559, 2009.
- A. A. A. Emara, B. A. El-Sayed, and E. S. A. E. Ahmed, “Syntheses, spectroscopic characterization and thermal behavior on novel binuclear transition metal complexes of hydrazones derived from 4,6-diacetylresorcinol and oxalyldihydrazine,” Spectrochimica Acta—Part A, vol. 69, no. 3, pp. 757–769, 2008.
- A. A. A. Emara and A. A. A. Abou-Hussen, “Spectroscopic studies of bimetallic complexes derived from tridentate or tetradentate Schiff bases of some di- and tri-valent transition metals,” Spectrochimica Acta—Part A, vol. 64, no. 4, pp. 1010–1024, 2006.
- H. S. Seleem, A. A. Emara, and M. Shebl, “The relationship between ligand structures and their CoII and NiII complexes: synthesis and characterization of novel dimeric CoII/CoIII complexes of bis(thiosemicarbazone),” Journal of Coordination Chemistry, vol. 58, no. 12, pp. 1003–1019, 2005.
- M. Otomo and I. Nakayama, “Solvent extraction and spectrophotometric determination of palladium(II) with some nitrogen-containing heterocyclic hydrazones in the presence of chloride ions,” Microchemical Journal, vol. 25, no. 1, pp. 75–81, 1980.
- T. Odashima and H. Ishii, “Synthesis and properties of hydrazones from 3- and/or 5-nitro-2-pyridylhydrazine and heterocyclic aldehydes, characterization of their complexes and extraction-spectrophotometric determination of traces of nickel with 2-pyridinecarbaldehyde 3,5-dinitro-2-pyridylhydrazone,” Analytica Chimica Acta, vol. 277, no. 1, pp. 79–88, 1993.
- M. Robert, X. S. Francis, and J. David, Spectrometric Identification of Organic Compounds, John Wiley & Sons, New York, NY, USA, 2005.
- L. Donald, M. Pavia Gary, and S. Lampman, Introduction to Spectroscopy, Thomson Learning, 3rd edition, 2001.
- E. Pretsch, P. Buhlmann, and C. Affolter, Structure Determination of Organic Compounds, Springer, Berlin, Germany, 3rd edition, 2001.
- D. P. Singh, R. Kumar, and J. Singh, “Synthesis and spectroscopic studies of biologically active compounds derived from oxalyldihydrazide and benzil, and their Cr(III), Fe(III) and Mn(III) complexes,” European Journal of Medicinal Chemistry, vol. 44, no. 4, pp. 1731–1736, 2009.
- S. Chandra and A. K. Sharma, “Nickel(II) and copper(II) complexes with Schiff base ligand 2,6-diacetylpyridine bis(carbohydrazone): synthesis and IR, mass, 1H NMR, electronic and EPR spectral studies,” Spectrochimica Acta—Part A, vol. 72, no. 4, pp. 851–857, 2009.
- M. Popovic, “Multinuclear NMR studies of alkali ions in nonaqueous solvents,” Pure and Applied Chemistry, vol. 51, no. 4, pp. 101–110, 1979.
- B. Ziyadanoǧullari, G. Topal, S. Erdoǧan, C. Hamamci, and H. Hoşgoren, “Effect of structural modifications of diaza-18-crown-6 on the extractability and selectivity of univalent metal picrates,” Talanta, vol. 53, no. 5, pp. 1083–1087, 2001.