Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo
Figure 4
Selective expansion of cotranduced murine bone marrow cells. 5-FU-enriched or SKL cells isolated from whole BM were cotransduced for 12 hours using equal or low MGMT : GFP virus mixtures at equivalent total MOIs. Single- and dual-expression levels in culture-expanded bone marrow (a) or SKL (b) cells before and after drug treatment. The percentage of cotransduced murine bone marrow (c) or SKL-derived, (d) CFU-surviving treatment with 20 μM BG and 0–30 μM BCNU. Error bars indicate ±SEM, .
