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ISRN Pharmaceutics
Volume 2012 (2012), Article ID 216068, 5 pages
Research Article

Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry

Department of Chemistry, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran

Received 23 October 2012; Accepted 7 November 2012

Academic Editors: J. Lee, L. Pezza, and C. Zacharis

Copyright © 2012 Mehdi Taghdiri and Arash Mohamadipour-taziyan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4–1.6 μg mL−1 (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% ( ). The detection limit was obtained, 0.26 μg mL−1 (1.7 μM). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.