Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry

Figure 4

Influence of stirring time on the absorbance of BCG in solid phase. Conditions: 1.2 μg mL⁻¹ of DP; [BCG] = 10⁻⁴ M; pH = 4.0; 70 mg Sephadex LH-20; sample volume 10 mL (air as reference). (a) before adding of Sephadex LH-20 and (b) after adding of Sephadex LH-20.

(a)

(b)