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ISRN Toxicology
Volume 2012 (2012), Article ID 247072, 10 pages
http://dx.doi.org/10.5402/2012/247072
Research Article

Toxicity Assessment of Expired Pesticides to Green Algae Pseudokirchneriella subcapitata

1Toxicology, International Institute of Biotechnology and Toxicology (IIBAT), Padappai, Kancheepuram District, Tamil Nadu 601301, India
2Bio-organic Chemistry, Central Leather Research Institute, Adayar, Chennai, Tamil Nadu 600 020, India

Received 26 August 2012; Accepted 1 October 2012

Academic Editors: F. Fernández-Piñas and G. J. McBean

Copyright © 2012 G. Satyavani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to investigate the effect of expired pesticides on the yield and growth rate of green algae Pseudokirchneriella subcapitata, a study was conducted as per the Organisation for Economic Cooperation and Development (OECD) guideline number 201. Fifteen expired pesticide formulations, most commonly used in Indian agriculture, were tested in comparison with their unexpired counterparts. The expired pesticide formulations studied belonged to various class and functional groups: organophosphate, pyrethroid-based insecticides; azole-based fungicides; acetamide, propionate, acetic acid-based herbicides; fungicides mixtures containing two actives—azole and dithiocarbamate. The toxicity endpoints of yield ( C50: 0–72 h) and growth rate ( C50: 0–72 h) of Pseudokirchneriella subcapitata for each pesticide formulation (both expired and unexpired pesticides) were determined statistically using TOXSTAT 3.5 version software. The results pointed out that some expired pesticide formulations exhibited higher toxicity to tested algal species, as compared to the corresponding unexpired pesticides. These data thus stress the need for greater care to dispose expired pesticides to water bodies, to avoid the effects on aquatic ecospecies tested.

1. Introduction

Agriculture is the back bone of world economy, and in India about 60% of the population depends on agriculture as their only occupation [1]. Pesticides have been a major contributor to the growth of agricultural productivity and food supply [2]. India ranks 10th position in the world in pesticide consumption as its total consumption amounts to about 500 million tons. India is presently the largest manufacturer of pesticides among the South Asian and African countries, with the exception of Japan. The Indian pesticides market is the 12th largest in the world with a value of US $0.6 bn, which is 1.6% of the global market pie [3]. Process of agricultural production is supported by the increased use of agrochemicals [4]. The increased use of pesticides will cause an increase in the number of pesticide dealers, including fumigators/pest controllers, retailers, and formulators [5]. Majority of dealers were reportedly interested in profit making and not pest control [6]. In India, obsolete pesticides have been either repacked or their date of expiry been erased and a new date of expiry given, and such pesticides are sold at a reduced price in the pesticide mela during festival season [7]. A recent case reported by Meenakam, World Tamils News Media, Hayleys Agro Chemical Company, Brahmanagama was caught while changing labels of expired pesticides from the date of expiry 2008 to 2012. On July 12, 2011, The Sri Lankan Consumer Affairs Authority raided the warehouse of the company and found 100,000 expired packets of pesticide, worth Rs. five million [8].

A case was reported at Nongpoh, Shillong, were packets of expired pesticides were distributed free of cost to farmers. According to the officer, the pesticide was supplied by M/s. ENBEE Enterprise of Shillong in the month of July 2010. However, the farmer received the expired pesticide in the month of March, 2011 [9]. Another case of selling expired pesticides illegally by a dealer at Punjab in 2002 was reported. The city police registered a case against Bhim Sain Goyal, a pesticide dealer and former president of the Mansa Fertilisers, Seeds and Pesticides Dealers Association for selling expired, substandard, and spurious pesticides. According to the First Information Report, the dealer had changed the date of manufacturing, expiry, and the price of pesticides. The pesticides had already expired on the date of sale to the farmer by the owner of Union Seed Agency [10]. Expired pesticides are the obsolete pesticides which are no longer used for original purpose and these obsolete pesticides are a major threat to human health and environment. There are stocks of pesticides that have lost their efficacy because their shelf life has expired. The expiry date indicates the endpoint when a chemical is no longer within acceptable specifications for its efficiency and stability [11]. The terms “expired” and “obsolete” pesticides may be used interchangeably as in practical terms the two situations are the same [12]. India has at least 200 tons of obsolete pesticides in terms of active ingredients [13]. In Nepal, the amount of expired pesticides was estimated as 150 tons; however, despite the date of expiry being reached, 39 tons were used in farming and 75 tons were sprayed without planning or buried [14].

Due to lack of awareness, farmers are using these expired pesticides which will enter water bodies through agriculture runoff. A study conducted in Rift Valley, Ethiopia, showed that 62% of farmers did not check the expiry date of the pesticides when they bought them and 10% of the farmers kept left-over pesticides and used them in the next season [15], which were expired when used. PAN UK reported that about 91% of the farmers prepared their pesticides close to water sources used by local people for drinking, cooking, and other household purposes. In all, 61% washed their pesticides sprayers and other equipment on the farm field [15] in Ethiopia. EPA in 1990 reported that 10% of community wells and 4% of rural domestic wells have detectable levels of at least one pesticide of the 127 pesticides tested in a national survey [16]. Along with this, obsolete pesticides are more likely to be spilt or leak into the environment and populations and the risk of exposure to these pesticides is higher [17]. Leaking of disposed date expired pesticides, runoff of sprayed chemicals, and improper disposing of empty containers are some of the sources which pose major threat to water contamination [18].

Algae are one of the essential living organisms of aquatic ecosystems. They produce oxygen and organic substances on which most other life forms depend by providing food for other organisms, including fish and invertebrates. Toxic chemical effects on algae can directly affect the structure and function of an ecosystem, resulting in oxygen depletion and decreased primary productivity [19], and also affect the food chain in the aquatic ecosystem. Natural ecosystems are complex and consist of many layers of interacting organisms. Damage to any one of these organisms in the ecosystem may have an impact on the entire ecosystem [20]. Pesticides can affect the structure and function of aquatic communities through altering species composition of an algal community [21]. Organophosphate pesticides and other pesticides (such as pyrethroid-based pesticides) used in urban and agricultural areas in the SJR (San Joaquin River) watershed and Delta are causing aquatic life toxicity in the state’s waters [22]. Pesticide mixtures in streams draining agricultural watersheds resulted in increased risk compared with consideration of individual pesticides to Pseudokirchneriella subcapitata [23]. As a threat reported to green algae, we focused on the toxicity of expired pesticides towards green algae Pseudokirchneriella subcapitata.

2. Objective

The purpose of the present study is to determine the toxicity of the expired and unexpired pesticide formulations towards Pseudokirchneriella subcapitata and compare them with their toxicological endpoints such as and and their 95% confidence limits. Growth is quantified with measurements of the algal biomass as a function of time.

3. Materials and Methods

Based on the wide usage of pesticide formulations in India, the following pesticide formulations were selected for the present study: Insecticides: (1) Dichlorvos 76% EC, (2) Endosulfan 35% EC, (3) Quinalphos 25% EC; Pyrethroids: (4) Alphacypermethrin 10% SC, (5) Fenvalerate 20% EC, (6) Lambda-cyhalothrin 5% EC; Herbicides: (7) Pretilachlor 50% EC, (8) 2,4-D sodium salt 80% WP, (9) Fenaxaprop-p-ethyl 9.3% EC;Fungicides: (10) Tebuconazole 25% EC, (11) Mancozeb 75% WP, (12) Hexaconazole 5% EC; Combinational Fungicides: (13) Captan 70% + Hexaconazole 5% WP, (14) Carbendazim 12% + Mancozeb 63% WP, (15) Metalaxyl 8% + Mancozeb 64% WP.

All the above products (unexpired pesticide formulation) were purchased from pesticide shops in the market, and all the products were within the mandatory for two-year-shelf life period. The same 15 expired products were obtained from IIBAT’s repository (expiry period within the range of 10 to 24 months after two years of shelf life) were tested simultaneously as given in Table 1. Manufacturer’s name and brand name of pesticides are withheld.

tab1
Table 1: Details of pesticide formulations.

The test species green alga Pseudokirchneriella subcapitata strain SAG 61.81 was used in this study. The primary culture was procured from University of Göttingen, Germany, and the culture is maintained in the Department of Ecotoxicology, IIBAT, as per the procedure stated in the OECD guideline number 201 [24].

The nutrient culture medium, OECD TG 201 medium (Table 2), was used for the present study. The deionised water was used to prepare OECD medium throughout the study. All pesticide formulations were dissolved in the OECD medium without using any solvent. The initial pH of bulk solution of each test item concentration and control was recorded and adjusted when required to using 0.1 M NaOH (sodium hydroxide) solution, and final pH was recorded in control and treated flasks at 72 h after incubation. The test item concentrations (Table 3) were fixed for each pesticide formulation both for expired and unexpired pesticide formulations on the basis of available reference literature for unexpired pesticide formulation [25]. The test item stock solution was diluted with the OECD medium to attain the required test item concentrations. For each treatment and control, 100 mL of test item solution was transferred into sterile 250 mL Erlenmeyer flasks.

tab2
Table 2: Composition of the OECD TG 201 medium.
tab3
Table 3: Percent inhibition of yield (% ) and growth rate (% ) at 72 hours.

The control and treatment flasks were inoculated with three-day-old preculture of Pseudokirchneriella subcapitata to get an initial cell concentration of about cells per mL under aseptic conditions. All the flasks were kept in the shaker incubator for 72 h providing continuous illumination of 6500–8000 lux light intensity with temperature ranging 20–22°C and continuously shaken at 110–120 rpm. During the experiment, control and treatment flasks were randomly repositioned in the shaker incubator daily to minimize the variation of the light intensity. After 72 h of incubation, alga cell count was recorded in all the flasks using Improved Neubaur’s Haemocytometer under illuminance of the microscope.

Based on the inhibitions of yield and growth rate, (0–72 h) and (0–72 h) were determined with 95% confidence limits by probit, logit, and log-log model. The ECX (effective concentration) values were reported based on the statistical model with the best fit. The calculations were done using software TOXSTAT 3.5 version [26].

4. Results and Discussion

In the present study, the biomass of Pseudokirchneriella subcapitata (cells/mL) in the control was increased exponentially and recorded more than 16 times in 72 h. The percent coefficient of variation (% CV) for section by section specific growth rate in the control during the whole test period did not exceed 35% and the percent coefficient of variation of average specific growth rate during the whole test period in the control did not exceed 7%. The final pH in the control did not vary by more than 1.5 units from the initial pH value.These above findings are validating the results of the present study (as per OECD guideline number 201, 2006). EC50 (EyC50 and ErC50) values were reported in Figures 15.

fig1
Figure 1: Effective yield and growth rate concentration of insecticides on Pseudokirchneriella subcapitata at 0–72 h.
fig2
Figure 2: Effective yield and growth rate concentration of pyrethroids on Pseudokirchneriella subcapitata at 0–72 h.
fig3
Figure 3: Effective yield and growth rate concentration of herbicides on Pseudokirchneriella subcapitata at 0–72 h.
fig4
Figure 4: Effective yield and growth rate concentration of fungicides on Pseudokirchneriella subcapitata at 0–72 h.
fig5
Figure 5: Effective yield and growth rate concentration of combinational fungicides on Pseudokirchneriella subcapitata at 0–72 h.

In expired condition (Table 4), insecticides and combinational fungicides were relatively less toxic when compared to the unexpired. The increased toxicity of expired pesticide formulations of pyrethroids, herbicides, and fungicides was attributed to the degraded products of active ingredients or impurities of the pesticide formulations which might be more toxic than their parent compounds, in similar low toxicity of insecticides and combinational fungicides when compared to the unexpired which may be due to less potent degraded products than their parent compounds. Along with this we have determined pH of the above pesticide formulations in expired and unexpired condition. pH of the above compounds was reported in the research article “Toxicity effect of expired pesticides to freshwater fish, Labeo rohita” [27]. Due to pH alteration there may be formation of unknown compounds which were not studied and there is a requirement for detailed investigation of those degraded products. The degree of toxicity depends upon the nature of the pesticides, their environmental concentration, and factors such as temperature, humidity, pH, and oxygen concentration [28]. The impurities may also contribute to the toxicity of the pesticide or might alter the physical properties of the product [29]. Ambrus et al. [29] reported that unfavorable storage conditions may lead to the decomposition of pesticides to produce degradation products much more toxic than the active ingredient. In agreement with Ambrus et al., Sanyal and Dureja [30] reported that Technical quinalphos stored in open glass bottle at 30°C for 6 months underwent degradation to give a black viscous mass containing the parent compound (C2H5O)2P(S)OQ (7.5%), isoquinalphos (C2H5O)2P(O)SQ (5.8 %), quinalphos oxon (C2H5O)2P(O)OQ (3.2%), 2-hydroxyquinoxalin QOH (12%), quinoxalin-2-thiol QSH (18%), diquinoxalin-2-yl-sulfide QSQ (15%), diquinoxalin-2-yldisulfide QSSQ (7%), dithienobisquinoxalin Q(S2)Q (2.0%), and at least 11 other compounds

tab4
Table 4: Toxicity level of expired pesticide formulations against unexpired in percentage (%).

From the above study, we found that expired insecticides (include organophosphate and cyclodiene) EC50 values were high which indicated less toxicity when compared with unexpired and this might be due to less potent degraded products which may be formed from the active ingredients or impurities. Similar to this was reported by Hamilton et al. [31], aldicarb sulfone and aldicarb sulfoxide, metabolites of aldicarb (insecticide) whose toxicity was altered (less potent and equipotent, resp.) from parent compound. Larson et al. [32] reported that endosulfan sulfate, a transformed product of endosulfan, is more toxic to fish than endosulfan. He also reported that 3-methyl-4-aminophenol and 1-naphthol, metabolites of fenitrothion and carbaryl (insecticides-organophosphate and carbamate), respectively, are more toxic to fish than their parent compounds. Allender and Keegan [33] reported that one 2-year-old sample containing 13.8% TCP, 0.65% sulfotep, and trace amounts of chlorpyrifos oxon was reported as the cause of the death of 50 bulls treated directly with the product for ecto-parasite control [33]. In our results, expired pyrethroids EC50 values were lower when compared with unexpired indicating increased toxicity of expired pesticides. Lee and Jones [22] reported that pyrethroid-based pesticides have been found to cause aquatic life toxicity in storm water runoff and other runoff/discharges from urban and agricultural areas where they have been applied. These pesticides accumulate in sediments following runoff events, causing sediment toxicity. In sediment toxicity there is an accumulation of dead algae, which create anoxic conditions in sediments through their decay. This leads to an accumulation of ammonia in the sediments, which is toxic to a number of forms of aquatic life. It also leads to low DO (dissolved oxygen) conditions, which are also toxic to many forms of aquatic life [22].

We found EC50 values of expired fungicides were lower which was due to higher toxicity against unexpired. In agreement with this, Shen [34] reported degradation product of fungicide chlordimeform, 4-chloro-methyl aniline, possesses strong carcinogenic effect towards rats than their parent substance. Herbicides in expired condition showed increased toxicity with low EC50 values in comparison to its counterpart. In contrast to this, Glenn [35] reported that atrazine, a herbicide, was significantly more toxic to green algae than its degradation products, deethylated atrazine, deisopropylated atrazine, hydroxy-, and diaminoatrazine. In agreement with our results, Battaglin and Fairchild [36] reported water in midwestern streams during spring and early summer runoff events can contain pesticides in sufficient quantities to be toxic to nontarget aquatic organisms and say that herbicide degradation can substantially increase the estimated toxicity of stream water to aquatic plants. Miller et al. [37, 38], through toxicity identification evaluation procedures, determined that a number of unidentified algal toxicants in Central Valley waters are likely to be herbicides. These herbicides can affect algal populations that in turn can affect DO depletion in the DWSC (deep-water ship channel) through reducing the magnitude of the algal-related oxygen demand. Based on the above data, we can understand that due to agriculture runoff pesticides able to cause algal toxicity which can also be possible in expired pesticide usage and agriculture runoff and unknown degraded products formed may further increase the toxicity level to algae and whole food chain can get disturbed and in turn leads to environment pollution.

5. Conclusion

We found that (0–72 h) and (0–72 h) values (derived statistically) of above-mentioned pesticide formulations were higher in expired condition (pyrethroids, herbicides, and fungicides) when compared to unexpired respective formulation. When we compare this entire data as a whole, it suggests us not to use, accumulate, or store expired pesticides as bulk or individual which causes toxicity to green algae after leakage into water and leads to disturbance in the food chain and causing environment pollution. Hence, we suggest that strict regulations may be followed to ensure that expired pesticides are never released in the environment and they are properly incinerated or disposed off without impairing the aquatic environment.

Acknowledgment

The authors are thankful to the Management of IIBAT for providing the facility.

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