Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form

Table 6

Summary of degradation products of emtricitabine and peak purity of spots of emtricitabine resolved.

Stress conditions	𝑅𝑓 values of degradation products	Peak purity data 𝑟(𝑆,𝑀), 𝑟(𝑀,𝐸)
Acid hydrolysis, 0.1 N HCl, refluxed for 3 h at 80°C	0.03, 0.09, 0.75	0.999868, 0.999808
Alkaline hydrolysis, 0.01 N NaOH, refluxed for 3.5 h at 80°C	0.03, 0.07, 0.71	0.998896, 0.998778
Oxidation (H2O2, 3% v/v), refluxed for 8 h at RT	0.07, 0.43	0.999982, 0.999852
Wet heat, refluxed for 5 days at 80°C	Not detected	0.999959, 0.999957
Dry heat, heated in oven at 80°C for 12 days	Not detected	0.999966, 0.999944
Day light, exposed to sunlight for 5 days	Not detected	0.999811, 0.999806