Fluorometric Determination of Drugs Containing α-Methylene Sulfoxide Functional Groups Using 𝑁¹- Methylnicotinamide Chloride as a Fluorogenic Agent

Table 4

Recovery data of 1–5 when assayed in standard solutions using the proposed method.

Drug	Claimed drug concentration	Recovered concentration*	% recovery	Mean % recovery ± S.D.	CV
1 μg/mL	1; 3; 12; 15; 20; 30; 40; 50	0.99; 3.01; 12.02; 14.99; 20.1; 30; 39.99; 49.89	99%; 100.3%; 100.16%; 99.9%; 100.5%; 100%; 99.9%; 99.8%	99.4±0.44	0.45%
2 ng/mL	50; 75; 200; 500; 1000; 1200	50.1; 74.4; 203; 500; 1004; 1199	100.2%; 99.2%; 101.5%; 100%; 100.4%; 99.9%	100.2±0.75	0.755%
3 ng/mL	100; 300; 1200; 1500	98; 301; 1195; 1509	98%; 100.3%; 99.6%; 100.6%	99.625±1.16	1.166%
4 ng/mL	100; 300; 400; 600; 800; 1000; 1200; 1500	99.5; 301; 403; 597; 799; 1002; 1198; 1493	99.5%; 100.3%; 100.75%; 99.5%; 99.87%; 100.2%; 99.83%; 99.53%	99.94±0.46	0.455%
5 ng/mL	20; 200; 600; 800; 1000; 1500; 2200	20.2; 202; 606; 795; 990; 1510; 2185	101%; 101%; 101%; 99.4%; 99%; 100.6%; 99.3%	100.5±0.92	0.92%

*Average of triplicate analyses for each concentration.