Characterization of LlaKI, a New Metal Ion-Independent Restriction Endonuclease from Lactococcus lactis KLDS4

Figure 1

SDS-PAGE analysis of the purification steps of LlaKI. Samples from different steps of the purification procedure were resolved on 12% polyacrylamide gel. Mr: prestained molecular weight marker, line 1: flow-through fraction of the DEAE cellulose column, line 2: Mono Q column fractions pool line 3: Mono S column fractions pool line 4: HiTrap Heparin HP fraction. The line indicates the LlaKI band.