International Scholarly Research Notices

Research Article

Feeding of Bait to Snail Lymnaea acuminata and Their Effect on Certain Enzyme in the Nervous Tissue

Table 2

Effect of sublethal exposure (20% and 60% of 24 h and 96 h LC₅₀) of bait formulations containing eugenol, ferulic acid, umbelliferone, and limonene on the activity of acetylcholinesterase (AchE) in the nervous tissue of the snail L. acuminata.


Treatment	24 h LC ₅₀		96 h LC₅₀		Withdrawal
Treatment	20%	60%	20%	60%	60% (96 h LC₅₀)

Control (Agar)	0.099 ± 0.002 (100)				0.097 ± 0.007 (100)
Control (a) Glu. + Meth.	0.098 ± 0.003 (100)				0.097 ± 0.005 (100)
Control (b) Star. + Meth.	0.097 ± 0.002 (100)				0.098 ± 0.006 (100)
Control (c) Glu. + Hist.	0.096 ± 0.005 (100)				0.095 ± 0.001 (100)
Control (d) Star. + Hist.	0.098 ± 0.004 (100)				0.097 ± 0.006 (100)
Glu. + Meth. + Eug.	0.075 ± 0.001* (75.75)	0.063 ± 0.002* (63.63)	0.05 ± 0.001* (58.58)	0.051 ± 0.003* (51.51)	0.067 ± 0.006⁺ (69.07)
Glu. + Meth. + Fer.	0.065 ± 0.003* (65.65)	0.051 ± 0.002* (51.51)	0.086 ± 0.003* (86.86)	0.065 ± 0.004* (65.65)	0.078 ± 0.003⁺ (98.73)
Glu. + Meth. + Umb.	0.073 ± 0.001* (73.73)	0.068 ± 0.008* (68.68)	0.070 ± 0.003* (70.70)	0.055 ± 0.001* (55.55)	0.063 ± 0.001⁺ (64.94)
Glu. + Meth. + lim.	0.078 ± 0.002* (78.78)	0.069 ± 0.001* (69.69)	0.067 ± 0.002* (67.67)	0.060 ± 0.003* (60.60)	0.075 ± 0.002⁺ (77.31)
Star. + Meth. + Eug.	0.055 ± 0.006* (55.55)	0.050 ± 0.003* (50.50)	0.058 ± 0.005* (58.58)	0.049 ± 0.006* (49.49)	0.055 ± 0.004⁺ (56.70)
Star. + Meth. + Fer.	0.062 ± 0.004* (62.62)	0.060 ± 0.008* (60.60)	0.058 ± 0.001* (58.58)	0.054 ± 0.003* (54.54)	0.059 ± 0.003⁺ (60.82)
Star. + Meth. + Umb.	0.072 ± 0.005* (71.71)	0.065 ± 0.001* (65.65)	0.062 ± 0.002* (62.62)	0.057 ± 0.005* (57.57)	0.062 ± 0.003⁺ (63.91)
Star. + Meth. + Lim.	0.072 ± 0.008* (72.72)	0.063 ± 0.004* (63.63)	0.068 ± 0.003* (68.68)	0.058 ± 0.002* (58.58)	0.062 ± 0.001⁺ (63.91)
Glu. + hist. + Eug.	0.057 ± 0.001* (57.57)	0.054 ± 0.002* (54.54)	0.055 ± 0.007* (55.55)	0.050 ± 0.007* (50.50)	0.058 ± 0.006⁺ (59.79)
Glu. + Hist. + Fer.	0.082 ± 0.003* (82.82)	0.075 ± 0.006* (75.75)	0.069 ± 0.003* (69.69)	0.061 ± 0.003* (61.61)	0.069 ± 0.003⁺ (71.13)
Glu. + Hist. + Umb.	0.070 ± 0.008* (70.70)	0.068 ± 0.001* (68.68)	0.069 ± 0.004* (69.69)	0.066 ± 0.002* (66.66)	0.070 ± 0.006⁺ (72.16)
Glu. + Hist. + Lim.	0.069 ± 0.003* (69.69)	0.066 ± 0.003* (66.66)	0.065 ± 0.007* (65.65)	0.060 ± 0.003* (60.60)	0.006 ± 0.003⁺ (68.04)
Star. + Hist. + Eug.	0.057 ± 0.006* (57.57)	0.055 ± 0.004* (55.55)	0.055 ± 0.003* (55.55)	0.051 ± 0.005* (51.51)	0.057 ± 0.008⁺ (58.76)
Star. + Hist. + Fer.	0.060 ± 0.003* (60.60)	0.059 ± 0.003* (59.59)	0.058 ± 0.002* (58.58)	0.055 ± 0.001* (55.55)	0.058 ± 0.002⁺ (59.79)
Star. + Hist. + Umb.	0.078 ± 0.008* (78.78)	0.077 ± 0.006* (77.77)	0.075 ± 0.001* (75.75)	0.070 ± 0.003* (70.70)	0.073 ± 0.006⁺ (75.25)
Star. + Hist. + Lim.	0.074 ± 0.003* (74.74)	0.071 ± 0.002* (71.71)	0.072 ± 0.002* (72.72)	0.068 ± 0.004* (68.68)	0.073 ± 0.003⁺ (75.25)

Each value is mean ± SE of six replicates. Values in parentheses are percent change with control taken as 100%.
Concentration (w/v) has been expressed as final concentration in aquarium water. *Significant (P < 0.05) when t-test was applied in between treated and control groups and ⁺in between 60% of 96 h LC₅₀ and withdrawal group. Glu.: glucose; Star.: starch; Meth.: methionine; Hist.: histidine; Eug.: eugenol; Feb.: ferulic acid; Umb.: umbelliferone; Lim.: limonene.