H19-Promoter-Targeted Therapy Combined with Gemcitabine in the Treatment of Pancreatic Cancer
Figure 4
The effect of sequential administration of BC-819 and gemcitabine on tumor progression and on the survival of a nude mice heterotopic pancreatic carcinoma model. Pancreatic carcinoma cells from hamster (PC1-0 cells) were injected subcutaneously into the back of athymic nude mice. After tumor development, 3 treatments of plasmid vectors were given, with a 2-day interval between each treatment, by direct injection into the tumor. Mice were randomly divided into 4 groups of 4 animals each and received the following treatments: Luc-H19 (control vector), BC-819, Luc-H19 + gemcitabine, BC-819 + gemcitabine. Mice were sacrificed when tumor reached a diameter larger than 13 mm. (a) Average tumor growth progression comparing the tumor volume measured after the last treatment to the volume measured before the first treatment. (b) Percentage of mice with a tumor diameter <13 mm as a function of time after the start of the treatment. The days or treatment are marked by arrows.
