Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro
Figure 5
Expression of (a) VR1412 β-gal, (b) GFP in KB cells and (c) KB cells expressing GFP seen with fluorescent microscopy (magnification × 10) Group 1: negative control (KB cells); Group 2: Naked DNA; Group 3: Ch-DNA nanoparticule with a Mw = 5 KDa chitosan; Group 4: Ch-PEG-FA-DNA nanoparticule with a Mw = 5 KDa chitosan; Group 5: Ch-DNA nanoparticule with a Mw = 25 KDa chitosan; Group 6: Ch-PEG-FA-DNA with a Mw = 25 KDa chitosan; Group 7: Ch-DNA with a Mw = 50 KDa chitosan; Group 8: Ch-PEG-FA-DNA nanoparticulewith a Mw = 50 KDa chitosan; Group 9: lipofectamine coupled with DNA; Group 10: KB cells seen with optical microscopy; Group 11: KB cells seen with fluorescent microscopy; Group 12: negative control, nontreated KB cells seen with fluorescent microscopy. *Statistical significant differences compared with positive control .
