Clinical Study
Immunohistochemical Evaluation of Cell Cycle Regulators: Impact on Predicting Prognosis in Stage T1 Urinary Bladder Cancer
Table 1
Antibodies for immunohistochemistry.
| | Antibodies | Clones | Source | Dilution
| Abnormal | Positive |
| | Cyclin D1 | P2D11F11 | Novocastra | 1 : 10 | >10% | Nuclei | | p53 | DO-7 | DAKO | 1 : 100 | >10% | Nuclei and cytoplasm | | p21 | SX118 | DAKO | 1 : 50 | <10% | Nuclei | | p16 | 6H12 | Novocastra | 1 : 20 | 0% or >50% | Nuclei and cytoplasm | | pRb | G3-245 | BD Pharmingen | 1 : 100 | 0% or >50% | Nuclei | | MMP 2 | 17B11 | Novocastra | 1 : 40 | >10% | Cytoplasm | | MMP 9 | 2c3 | Novocastra | 1 : 40 | >10% | Cytoplasm |
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