| Isolates |
Before En | After En | 1st En | 2nd En | 3rd En | 4th En |
| Asph1 Gram +ve bacillus shape, straight rods with rounded ends, opaque, smooth, convex, entire, and mutt creamy white | (+++) | (+++) | (+++) | (+++) | (++) | Asph2 Gram −ve rod-shaped, straight or slightly curved rods, irregular margin, opaque, smooth, low convex, entire, and shiny beige | (+++) | (+++) | (+++) | (++++) | (++++) | Asph3 Gram +ve cocci shape, small, circular, opaque, smooth, convex, entire, and yellow canary color | (++) | (++) | (++) | (++) | (+++) | Asph4 Gram +ve short-rod-shaped, small, straight, or slightly curved rods, opaque, smooth, convex, entire, and orange color | (+) | (+) | (+) | (−) | (−) |
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