Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays
Figure 1
Characterization of SiO2 NP size and stability. (a) Representative transmission electron micrograph of SiO2 NP. Arrow points out representative SiO2 NP. Scale bar is equal to 100 nm. (b) Particle size distribution of SiO2 NP measured by ES-DMA (raw counts). (c) SiO2 NPs were diluted in water (38 g/L-39 g/L) and characterized by DLS. SiO2 NP display an average size of ~7 nm in water with some agglomeration also noted (~50 nm). A representative intensity-weighted histogram is shown. (d) SiO2 NP were diluted in DMEM media supplemented with FBS and penicillin/streptomycin, incubated at 37°C for 30 minutes (black thin line), 60 minutes (black thin punctuated line), 3 h (black thick line), or 24 h (gray punctuated line), and 3 measurements were run at each time point. Media only (gray thick line) is also included. One representative intensity-weighted histogram is shown at each time point. SiO2 NP average diameter is 20 nm at 30 min and 60 min and increases to ~60 nm at 3 h.
