Co-localization of the PDGF β-Receptor and Actin during PDGF Stimulation in Mouse Fibroblasts

Figure 2

Immunofluorescence labeling revealing the localization of the PDGF β-receptor in serum-starved cells. Serum-starved mouse fibroblasts were stained with two different antibodies raised against the PDGF β-receptor (green) and stained for F-actin with phalloidin-TRITC (red). The antibody raised against a peptide corresponding to amino acids 1013–1025 of human PDGF β-receptors located the PDGF β-receptor in patches on the cell surface in serum-starved cells (a, arrows). Patches with receptors are often located at the edge of cells. The patches with PDGF β-receptors were also detected with the antibody raised against a peptide that corresponds to part of the extracellular domain of murine PDGF β-receptors in serum-starved cells (d, arrows). In addition a more homogenous scattered distribution of PDGF β-receptors was detected with both antibodies (a, d). Bar represents 10 μm.