Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves

Table 2

Elution methods for the three systems are specified. Solvent A: water/acetic acid (98 : 2); solvent B: methanol/acetic acid (98 : 2).

Particulate C-18 column		Monolithic column		UPLC system
Time (min)	Solvent B (%)	Time (min)	Solvent B (%)	Time (min)	Solvent B (%)
0	0	0	0	0	0
5	30	2	32	2	15
20	30	5	32	3	60
30	50	8	40	4	60
40	100	9	100	4.1	100