Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves

Table 4

Repeatability and reproducibility of the developed methods.

	Compounds
	1	2	3	4	5
HPLC with particulate C-18 column
RT (min)	7.9	9.0	25.1	29.0	30.7
RT (RSD, 𝑛=12, intraday)	0.49	0.64	1.39	0.54	0.40
RT (RSD, 𝑛=18, interday)	0.38	0.49	1.12	0.43	0.32
Area (RSD, 𝑛=12, intraday)	5.73	6.37	5.70	5.74	5.19
Area (RSD, 𝑛=18, interday)	5.38	6.19	5.81	5.07	4.39
Asymmetry	1.98	0.94	1.20	1.36	0.80
HPLC with monolithic columns
RT (min)	1.38	1.86	3.98	4.41	4.86
RT (RSD, 𝑛=12, intraday)	1.18	0.61	0.38	0.62	0.71
RT (RSD, 𝑛=18, interday)	1.07	0.77	0.96	1.45	1.58
Area (RSD, 𝑛=12, intraday)	0.64	3.02	0.99	0.79	0.57
Area (RSD, 𝑛=18, interday)	1.17	3.79	1.78	1.85	2.00
Asymmetry	1.31	1.29	1.16	1.43	1.63
UPLC
RT (min)	1.24	1.86	3.40	3.63	3.81
RT (RSD, 𝑛=12, intraday)	0.13	0.07	0.04	0.06	0.07
RT (RSD, 𝑛=18, interday)	0.25	0.22	0.04	0.05	0.06
Area (RSD, 𝑛=12, intraday)	0.25	0.35	0.77	0.26	0.18
Area (RSD, 𝑛=18, interday)	0.63	0.82	0.77	0.28	1.28
Asymmetry	1.80	2.02	1.29	1.87	1.01