Simultaneous Determination of Six Major Constituents of the Herbal Formula Insampaedok-san Using HPLC-PDA

Table 2

Linearity, correlation coefficient, LOD, and LOQ of the marker compounds (𝑛=3).

Compound	Linear range (μg mL^−1)	Regression equation^a	Correlation coefficient (𝑅^2)	LOD^b (μg mL^−1)	LOQ^c (μg mL^−1)
1	0.78–100.00	𝑌 = 16,546.59𝑥 + 327.71	1.0000	0.09	0.29
2	0.39–50.00	𝑌 = 46,824.31x − 4,190.75	1.0000	0.03	0.09
3	3.13–400.00	𝑌 = 15,866.42𝑥 + 3,406.43	1.0000	0.08	0.27
4	0.39–50.00	𝑌 = 18,561.95x − 244.18	1.0000	0.08	0.26
5	1.56–200.00	𝑌 = 19,357.24𝑥 + 1,447.17	1.0000	0.07	0.22
6	0.78–100.00	𝑌 = 8,513.98𝑥 + 1,124.60	1.0000	0.09	0.31

^a𝑌 = peak area (mAU) of the components. 𝑥 = concentration (μg mL⁻¹) of the components.
^bLOD = 3 × signal-to-noise (S/N) ratio.
^cLOQ = 10 × signal-to-noise (S/N) ratio.