- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Volume 2012 (2012), Article ID 616310, 14 pages
Development of Safer Gene Delivery Systems to Minimize the Risk of Insertional Mutagenesis-Related Malignancies: A Critical Issue for the Field of Gene Therapy
Department of Biology, College of Science and Technology, Temple University, Bio-Life Science Building, Suite 456, 1900 N. 12th Street, Philadelphia, PA 19122, USA
Received 29 August 2012; Accepted 23 October 2012
Academic Editors: A. Goussia, T. Otto, and Z. Suo
Copyright © 2012 Gaetano Romano. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Integrating gene delivery systems allow for a more stable transgene expression in mammalian cells than the episomal ones. However, the integration of the shuttle vector within the cellular chromosomal DNA is associated with the risk of insertional mutagenesis, which, in turn, may cause malignant cell transformation. The use of a retroviral-derived vector system was responsible for the development of leukemia in five children, who participated in various clinical trials for the treatment of severe combined immunodeficiency (SCID-X1) in France and in the United Kingdom. Unfortunately, the hematological malignancy claimed the life of one patient in 2004, who was enrolled in the French clinical trial. In addition, adeno-associated-viral-(AAV-) mediated gene transfer induced tumors in animal models, whereas the Sleeping Beauty (SB) DNA transposon system was associated with insertional mutagenesis events in cell culture systems. On these grounds, it is necessary to develop safer gene delivery systems for the genetic manipulation of mammalian cells. This paper discusses the latest achievements that have been reported in the field of vector design.
Gene transfer technology requires the introduction of recombinant genetic elements into human cells and holds a considerable therapeutic potential for the treatment of a wide variety of pathological conditions, such as cancer, genetic disorders, neurological illnesses, diabetes, infectious diseases, and cardiovascular maladies [1–7]. The gene-based treatments both of cancer and infectious diseases may require only a transient expression of the recombinant genetic elements, which have the function to destroy either neoplastic tissues, or cells that harbor an infectious agent [3, 4]. Conversely, genetic disorders, neurological illnesses and cardiovascular maladies need a long-term transgene expression, as the treatment of these maladies envisions the introduction of functional copies of certain genes in the attempt to correct the phenotype of the disease [3, 4]. A long-term transgene expression is also required for autologous T lymphocytes that are genetically engineered to express recombinant T cell receptors, which may impart binding specificity either for neoplastic markers or infected cells [5–7]. To this end, gene delivery systems that integrate their genome into the target cell chromosomal DNA allow for a more stable and long-lasting transgene expression [3, 4]. Various types of gene delivery systems are currently available [3, 4, 8–16]. The most common gene transfer models derive from retroviruses, lentiviruses, adenoviruses, adeno-associated viruses (AAV) and so-called non-viral vector systems [3, 4, 8–16]. Adenoviruses and most of nonviral-derived vectors are episomal gene transfer models [3, 4, 13, 14], whereas vectors based on retroviruses, lentiviruses, AAV, Sleeping Beauty (SB) DNA transposon system, and Steptomyces bacteriophage integrase ΦC31 have integrative properties [3, 4, 12, 14–16]. Sleeping Beauty (SB) DNA transposon system and Streptomyces bacteriophage integrase ΦC31 are non-viral-derived gene transfer systems that only require a plasmid DNA transfection for the delivery of their transgene, which is then stably integrated within the target cell genome .
Retroviral-based vector systems were utilized in the first two phase I gene therapy clinical trials, which were conducted in the United States of America between 1989 and 1990 [3, 4, 8–10]. One clinical trial dealt with the treatment of adenosine deaminase (ADA) deficiency, which is a genetic disorder leading to immunodeficiency [3, 4, 9, 10]. A retroviral vector carrying the functional copy of adenosine deaminase was used for the ex vivo gene transduction of autologous bone marrow-derived hematopoietic stem cells, which were subsequently reinfused back into the two young patients who were enrolled in this trial [3, 4, 9, 10]. The other clinical study used a retroviral-encoded neomycin resistance gene to trace autologous human tumor-infiltrating T lymphocytes in five patients with advanced melanoma [3, 4, 8–10]. Both gene therapy clinical trials demonstrated to be feasible and safe in patients. Moreover, a long-term clinical benefit was reported in the two young patients who participated in the first human gene therapy clinical trial for the treatment of ADA deficiency. The functions of the immune system were restored in both patients [3, 4, 9, 10]. Remarkably, the expression of the recombinant ADA gene was observed in 20% of lymphocytes of one patient ten years after the last infusion of transduced bone-marrow-derived hematopoietic stem cells . Naturally, this initial success prompted for the worldwide submission of hundreds of phase I and phase II human gene therapy protocols, which utilized various viral and nonviral gene delivery models for the treatment of monogenic disorders and cancer [3, 4]. However, the majority of these clinical trials provided disappointing results, as the design of the various gene transfer systems were not sufficiently adequate to support efficacious human gene therapy protocols in the clinical setting [3, 4]. Common problems were related to transgene silencing following the genetic manipulation of target cells and/or mediocre transduction efficiency [3, 4]. In addition, host immune responses to the vector systems and/or transduced cell populations constituted a very critical issue both in terms of safety and efficacy for the gene-based interventions [3, 4, 11–15, 17]. A young patient affected by a partial ornithine transcarbamylase deficiency died because of an acute inflammatory reaction, which was caused by a massive intrahepatic infusion of adenoviral vector particles carrying the functional copy of the defective gene [3, 13, 18]. This gene-based clinical trial was conducted in the United States of America in September 1999 and represented the first severe setback for gene therapy programs, which underwent austere public scrutiny [3, 13, 18]. Therefore, the field of vector design had to tackle a variety of critical issues to control host humoral and/or cytotoxic T cell (CTL) immune responses against viral vector particles and/or transduced cell populations [3, 4, 11–15, 17]. Furthermore, it was necessary to improve the production of viral and non-viral vector stocks, enhance the efficiency of ex vivo and in vivo gene transduction of several cell types, optimize transgene expression levels after gene transduction, and stabilize the duration of transgene expression in target cells [3, 4, 11–15, 17]. On these grounds, viral promoters were genetically modified in order to minimize de novo methylation of CpG-rich islands, which is one of the factors that are responsible for transgene silencing [3, 4, 11–15, 17]. Stronger enhancers were utilized to sustain higher levels of transgene expression in transduced cell populations [3, 4, 11–15, 17]. Lastly, protocols for the in vitro gene transduction of human hematopoietic stem cells were optimized [11–17]. These strategies contributed to the development of more efficient vector systems for gene transfer modalities both in human gene therapy protocols and preclinical studies [11–17]. Optimized gene transfer protocols were utilized in the field of stem cell research for the genetic manipulation of human and mouse embryonic stem (ES) cells, several types of adult stem cells, and, more recently, the production of induced pluripotent stem (iPS) cells, which may derive from every type of either human or animal somatic cell [19–33]. Indeed, gene transfer technology has emerged as a very useful tool to support stem cell research over the last decade [18–32]. The merging of gene transfer technology and stem cell research may have important implications in the field of regenerative medicine, which is aiming at developing novel therapeutic approaches for the treatment of neurodegenerative diseases, diabetes, and cardiovascular disorders [19–33]. So far, the combination between gene therapy and stem cell research was applied for the treatment of hematological maladies that derive from genetic disorders, such as ADA deficiency and severe combined immunodeficiency (SCID)-X1 [1–4].
On one hand, the integration of the shuttle vector within the chromosomal DNA of the target cell is one of the requirements for a long-term transgene expression. On the other hand, however, integrative gene transfer models have a potentially dangerous downside, as they are associated with the risk of insertional mutagenesis, which may eventually result in the development of malignancies [3, 4, 11, 12, 14, 15, 17]. The integration of the shuttle vector may tamper with the natural chromosomal arrangement of the target cell and trigger a variety of events leading to the establishment of a transformed cell phenotype [3, 4]. Unfortunately, retroviral-mediated gene transfer was responsible for the development of leukemia in five young subjects who participated in clinical trials for the treatment of SCID-X1 in France and in the United Kingdom [1, 2, 34–44]. The various outcomes reported in the SCID-X1 clinical trials and the mechanisms leading to the development of insertional mutagenesis-induced carcinogenesis will be discussed in greater detail in the next section. This paper will also describe the current strategies adopted to minimize and possibly prevent insertional mutagenesis-related events in patients.
2. Mechanisms of Insertional Mutagenesis-Induced Carcinogenesis in the SCID-X1 Clinical Trials
SCID-X1 is an inherited genetic disorder that leads to immunodeficiency and is associated with the loss of the common γ chain (γc) cytokine receptor subunit [36, 45, 46]. The absence of the γc subunit impairs the biological and biochemical functions of the cellular receptors that bind a variety of interleukins, such as IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 [45, 46]. The impaired cellular receptors fail to interact with a variety of interleukins, which are important regulators of cell proliferation, growth, survival, and differentiation [42, 43]. For this reason, early lymphoid progenitors become unresponsive to the previously mentioned interleukins and, for this reason, they are not able to differentiate into B, T, and natural killer (NK) cells [45, 46]. The deprivation of these three cell types in the hematopoietic compartment results in a severe host immunodeficiency [45, 46].
A retroviral-encoded γc subunit was used in the various gene therapy clinical trials for the treatment of SCID-X1, which were initially conducted in France  and then in the United Kingdom [42, 47], Australia , and United States of America . Beneficial clinical outcomes were reported in all of these gene-based trials [42, 47–50]. Autologous CD34+ bone marrow-derived cells were extracted from the patients and transduced in vitro with a retroviral-based vector system. The retroviral vector transduction was very efficient and the expression of the recombinant γc subunit rendered autologous CD34+ bone-marrow cells susceptible to the influence of IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 . The genetically modified autologous bone-marrow cells were reinfused into the patients, in the attempt to correct the phenotype of the disease. The French clinical trial was conducted in 2000 and initially reported a considerable success, as the immune system functions were restored in 9 out of 10 patients . However, four of these patients developed a leukemia-like illness in the following months [1–3, 34–44]. The hematological malignancy was first observed in two patients after periods of 30 and 34 months [1–3, 37, 43], whereas the other two patients came down with leukemia in subsequent months [2, 34, 36, 43]. Sadly, one of the first two patients who developed leukemia had a relapse of the disease and died in October 2004 [2, 38, 39]. An additional fifth case of leukemia was reported in a child who participated in the British SCID-X1 trial that was conducted in 2005 . The British and the Australian gene therapy clinical trials utilized lower amounts of transduced autologous CD34+ hematopoietic stem cells for infusion into the patients, with the intent to reduce the risk of insertional mutagenesis-induced malignancies [42, 47, 48]. Unfortunately, this strategy was not particularly effective, as shown by the fifth case of leukemia reported in the British SCID-X1 clinical trial .
There are two main mechanisms leading to insertional mutagenesis for retroviridae-based gene delivery models, which comprise retroviral- and lentiviral-derived vector systems [3, 4, 11, 15, 51]. The genera of retroviridae have two long terminal repeats (LTRs) situated at the 5′- and 3′-end of their genome (Figure 1). Each LTR contains an enhancer and promoter elements . Consequently, following the integration of the retroviridae-based vector into the cellular chromosomes, the 3′-LTR might promote the expression of an endogenous oncogene if this should be situated in the proximity of the integration site [3, 4, 11, 15]. Furthermore, the integration of the retroviridae-based vector might silence the expression of cellular tumor suppressor genes if the viral vector integration should occur within an exon of a certain tumor suppressor gene (Figure 2) [3, 4, 11, 15]. This first mechanism that may activate the expression of cellular oncogenes and/or silence tumor suppressor genes has a short range of action. A second mechanism for insertional mutagenesis with a much longer range of action envisions the interaction between the enhancer element of a retroviral LTR and a cellular promoter driving the expression of an endogenous oncogene (Figure 3) [3, 4, 11, 15]. The second mechanism was responsible for the onset of hematological malignancies in five patients of the SCID-X1 gene therapy clinical trials [1, 2, 34–44]. The analysis of malignant cells obtained from the first two leukemic patients of the French SCID-X1 clinical trial showed an overexpression of the LIM only protein 2 (LMO2) oncogene [37, 46]. LMO2 at physiological levels is an important regulator of hematopoietic stem cell development, whereas aberrant levels of LMO2 expression in T cells are associated with acute lymphoblastic leukemia [46, 52–54], whereas the possible involvement of LMO2 overexpression in B-cell acute lymphoblastic leukemia is currently under investigation . A report analyzed the malignant T cells obtained from the first two patients who developed leukemia in the French gene therapy clinical trial for the treatment of SCID-X1 . In one patient, the site of integration of the retroviral vector was 3 kb upstream of the LMO2 transcription start, whereas in the other patient the integration site was in antisense orientation to the LMO2 promoter and 5 kb downstream of the LMO2 transcription site, which corresponded to the first LMO2 intron . This study did not find any replication-competent retrovirus and ruled out a possible contribution of the overexpressed recombinant γc subunit to the onset of the hematological malignancy . According to a couple of reports, an overexpressed recombinant γc subunit induced tumors in animal models [56, 57]. However, further studies on human cells excluded the possibility that the overexpressed recombinant γc subunit had any carcinogenic effects [43, 58–60]. These findings, taken together, indicate that animal models do not always have the ability to recapitulate human maladies, especially in the case of oncological diseases [61–63].
LMO2 overexpression, per se, might not be sufficient to promote carcinogenesis, which requires a multistep mechanism that involves a variety of genetic alterations, epigenetic mutations, activation of cellular oncogenes, and/or inactivation of putative tumor suppressor genes and environmental factors [61–72]. A subsequent study analyzed the phenotype of malignant cells of the other two leukemic patients who participated in the French SCID-X1 clinical trial . LMO2 overexpression was reported also in these two clinical cases. Moreover, a second retroviral vector integration site was detected in the proximity of the proto-oncogene CCDN2 in leukemic cells of one patient, whereas malignant cells of the other patient carried an integrated retroviral vector close to the proto-oncogene Bmi1 . Blast cells of both leukemic patients exhibited a variety of genetic alterations, such as deletion of tumor suppressor gene cyclin-dependent kinase 2A (CDKN2A), chromosomal translocations, SIL-TAL1 rearrangements, 6q interstitial losses, and gain-of-functions resulting in the activation of NOTCH1 .
Almost analogous findings were reported in the phenotypic analysis of blast cells of the leukemic patient who was enrolled in the British SCID-X1 gene therapy clinical trial . LMO2 overexpression was observed also in this case, along with a variety of genetic aberrations, which included loss of expression of the tumor suppressor gene CDKN2A, gain-of-function of NOTCH1, and translocation of the T-cell receptor (TCR)-b region to the STIL-TAL1 locus .
A protocol based on linear amplification-mediated PCR (LAM-PCR) was developed to analyze the retroviral vector integration sites within the human genome following a gene-based intervention in patients [73–76]. The LAM-PCR protocol was used to determine the integration site profile of retroviral-based vectors within the genome of CD34+ hematopoietic stem cells of two patients with Wiskott-Aldrich syndrome (WAS), who participated in a phase I gene therapy clinical trial [76–78]. WAS is a genetic disorder characterized by micro-thrombocytopenia, frequent infections, eczema and is associated with a high incidence of lymphoreticular malignancy and autoimmune disorders . The retroviral vector system was utilized to express the functional copy of the human WAS gene in autologous CD34+ hematopoietic stem cells, which were transduced ex vivo and then injected back into the patients [76, 77]. LAM-PCR analysis was conducted 892 and 891 days after intervention in patients 1 and 2, respectively. This analysis reported 5,709 and 9,538 unique retroviral vector integration sites in patients 1 and 2, respectively. The most recurrent common integration sites (CIS) involved the following genetic loci: LMO2, MDS-EVI1, CCDN2 and PRDM16 [76, 77]. Retroviral integration sites either in the proximity or inside the LMO2 and CCDN2 gene loci were more frequent in lymphoid cells, whereas retroviral integration sites involving either the PRDM16 and MDS1-EVI1 gene loci were predominant in myeloid cells [76, 77]. The reconstitution of the hematopoietic compartment in both patients exhibited polyclonal blood cell populations, which indicates the absence of hematological malignancies [76, 77].
Further studies are currently in progress in order to better characterize the mechanism of insertional mutagenesis-induced hematological malignancies in patients. Indeed, the characterization of retroviral vector integration sites in dominating monoclonal blood cell population might reveal important clues on the deregulated cellular signaling systems, which may play a role in the establishment of a malignant cell phenotype and impart clonal expansion .
3. Preclinical Studies for Insertional Mutagenesis
The use of retroviridae-derived vector systems in clinical trials poses a serious safety concern, because of the onset of hematological malignancies that may be promoted by insertional mutagenesis events [1–3]. As already mentioned, insertional mutagenesis-induced malignancies were reported in five leukemic patients of the SCID-X1 gene therapy clinical trials [1–3, 34–44, 51, 81]. Insertional mutagenesis events were also observed both in animal systems [44, 58, 82–84] and human cell culture models [84–91]. These preclinical studies focused on the integrating properties of retroviridae-derived vector systems based on murine leukemia virus (MLV), avian sarcoma-leukosis virus (ASLV), and human immunodeficiency virus type 1 (HIV-1) [85–92]. The integration of the aforementioned retroviridae-derived vector systems was not completely random within the human genome. In fact, most of integration sites were preferentially localized in the proximity of chromosomal regions that contained transcriptionally active genes [85–91]. In addition, MLV-derived vector systems showed a remarkable predisposition for integration points near transcription start sites [85, 86], whereas HIV-1-based lentiviral vector systems favored genomic loci rich in active genes and intercalated with chromosomal regions containing methylated CpG islands that are not permissive for gene expression [85–89]. In contrast, ASLV-based vector systems did not exhibit any specific preference for transcription start sites and had no bias for regions containing active genes .
All integrating gene delivery models may present the risk of insertional mutagenesis in target cells. In fact, experiments in animal systems and cell culture models demonstrated that insertional mutagenesis might also be triggered by AAV-based vectors  and integrating non-viral vector systems, such as SB DNA transposon-derived vectors . In vivo administration of AAV-derived vectors was responsible for either insertional mutagenesis-induced angiosarcomas or hepatocellular carcinomas in mice [12, 92]. Other studies on human cell culture systems detected residual promoter-like properties in terminal repeat sequences of SB DNA transposon-derived vectors, which was able to induce the expression of cellular genes that were contiguous to the integration site of the non-viral vector system .
Many studies placed an emphasis on integration of retroviral- and AAV-derived vector systems within common fragile sites of the human genome [90, 94–96]. Human common fragile sites consist of specific regions linked with chromosomal breakpoints, which may play a relevant role in the early stages of malignant cell transformation [95, 97–102]. Interestingly, a number of human oncogenic viruses integrate preferentially their genome within common fragile sites . These human oncogenic viruses comprise human papilloma virus [103–106], Epstein-Barr virus [95, 107, 108], and hepatitis B virus .
The analysis of so-called hotspots for certain viral-derived vector systems integration sites is essential to determine the mechanism of insertional mutagenesis-related onset of malignancies in patients.
4. Tactics to Reduce the Incidence of Insertional Mutagenesis
Gene therapists are currently addressing the issue of insertional mutagenesis-induced malignancies in patients by pursuing three main approaches: (i) engineering of safer integrating gene delivery models; (ii) development of episomal gene delivery systems with improved duration of transgene expression in transduced cell populations; (iii) production of genetically modified meganucleases and zinc-finger proteins for the correction of genetic defects.
The central issue for the development of safer integrating gene delivery systems consists of producing gene transfer shuttle vectors that do not affect the physiological genomic organization of transduced cells. Therefore, it is necessary to prevent interactions between enhancer and/or promoters of the vector system and the host genome. The engineering of self-inactivating retroviridae-derived vectors may minimize the incidence of insertional mutagenesis-related events in transduced cells [3, 11, 15]. The replication cycle of the genera of retroviridae comprises an initial transcription stage, in which the viral messenger RNA (mRNA) is synthetized and subsequently packaged into the virion (Figure 4). This viral mRNA does not contain the enhancer/promoter U3 region in the 5′-LTR and the U5 region in the 3′-LTR. After cell infection, the viral mRNA is released into the cytoplasm and then is reversed transcribed into double stranded proviral DNA. At this stage, the U3 region in the 3′-LTR is copied at the 5′-LTR, whereas the U5 region in the 5′-LTR is copied at the 3′-LTR. The double-stranded proviral DNA is successively assembled in a preintegration complex, which crosses the nuclear membrane and is integrated within the genome of the infected cell (Figure 4) [3, 11, 15].
Self-inactivating retroviridae-derived vectors are produced by a deletion of the U3 region in the 3′-LTR (Figure 5) [3, 11, 15]. Thus, the 3′-LTR is no longer transcribing. In this context, the 5′-LTR drives the transcription of a viral mRNA lacking the U3 region in the 3′-LTR. Following the reverse transcription process in transduced cells, the truncated U3 region in the 3′-LTR is reproduced at the 5′-LTR. This results in a proviral DNA without transcriptional activity at both LTRs (Figure 5). Obviously, self-inactivating retroviridae-derived vectors need an internal promoter to express the transgene (Figure 5) [3, 11, 15].
Self-inactivating retroviridae-derived vectors may be considered safer than their original counterparts, which are termed gamma-retroviral or gamma-lentiviral based vectors [3, 11, 15]. The deletion of retroviral enhancers and promoter regions in the LTRs minimizes the probability of interactions between the transfer vector system and the host genome [3, 11, 15]. In fact, the nontranscribing 3′-LTR is not able to drive the expression of cellular oncogenes that might be present in the vicinity of the integration site (Figure 5). Moreover, the removal of retroviridae enhancer regions precludes the long-range interference with cellular promoters that control the expression of endogenous oncogenes (Figure 5) [3, 11, 15]. However, the presence of an enhancer within the internal promoter of a self-inactivating retroviridae-derived vector still poses the issue of insertional mutagenesis, as it may stimulate cellular promoters of oncogenic factors [3, 11, 15, 110, 111]. Studies produced self-inactivating lentiviral vectors containing enhancer-less internal promoters [110, 111]. In one case, a self-inactivating lentiviral vector system utilized as internal promoter the enhancer-less and methylation-free CpG islands promoter of the ubiquitously acting chromatin opening elements, which is a human housekeeping gene and was termed A2UCOE [110, 112]. The use of the A2UCOE internal promoter has a dual advantage: the first advantage is related to the vector design safety improvement that is due to the absence of enhancer regions; the second advantage is correlated with the absence of CpG-rich islands within the sequence of the human housekeeping gene promoter, which confers resistance to de novo methylation-mediated transcriptional silencing [110, 112]. The self-inactivating retroviridae-derived vector containing the enhancer-less A2UCOE internal promoter was utilized for the gene transduction of the recombinant γc subunit in primary bone-marrow-derived CD34+ hematopoietic stem cells of a patient with SCID-X1 . Transduced cell populations readily expressed the recombinant γc subunit and became susceptible to the action of interleukins in the in vitro system . Similar results were observed in a SCID-X1 mouse model . These findings indicate that the production of enhancer-less self-inactivating retroviridae-derived vectors constitute a significant improvement in safety design.
In other studies, the internal promoters of self-inactivating lentiviral vector systems were based either on the enhancer-less human Vav1 promoter [111, 113] or the phosphoglycerate kinase (PGK) promoter . The enhancer-less human Vav1 promoter was used for the expression of the recombinant γc subunit in a murine model of SCID-X1 . Although the transgene expression levels were not high, it was possible to correct the phenotype of the disease in a mouse model .
Another preclinical study on Fanconi anemia utilized the enhancer-less human Vav1 promoter and the phosphoglycerate kinase (PGK) promoter . Fanconi anemia is an inherited genetic disorder leading to bone marrow failure and high incidence of leukemia . Genotypic analysis of patients with Fanconi anemia identified mutations in a group of fifteen genes, which were termed FANC genes . Mutations in the so-called FANCA gene are among the most frequent in patients with Fanconi anemia . Self-inactivating lentiviral vector system was utilized in a preclinical study to express the functional copy of FANCA, which was driven either by the enhancer-less human Vav1 promoter or the phosphoglycerate kinase (PGK) promoter . Transduction efficiency and duration of transgene expression were efficient in this study. However, the levels of transgene expression were rather low. In order to optimize FANCA expression levels, the investigators utilized a variant of the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) region . Overall, all tested vector systems induced a comparable phenotypic correction in cell culture models for Fanconi anemia .
A self-inactivating lentiviral vector was utilized in a mouse model for the correction of SCID-X1. This vector system used a minimal promoter obtained from the eukaryotic elongation factor α (EF1α) gene to drive the expression of a codon-optimized human γc cDNA . Remarkably, this vector system was able to correct the phenotype of the disease in the mouse model, without causing the overexpression of the LMO2 oncogene . In contrast, the self-inactivating lentiviral vector system containing the human γc promoter driving the expression of the human γc cDNA induced the over-expression of LMO2 . The chicken hypersensitivity site 4 (cHS4) insulator was placed in the 3′-LTR of both vector systems . Insulators were utilized to prevent the silencing of retroviridae-encoded transgenes, which may derive either from de novo methylation of viral promoters and/or position effects imparted by chromosomal sequences surrounding the retroviral integration site [116, 117]. However, the result of this study indicates that insulators are not efficacious in providing a barrier against the genotoxicity of retroviridae-based vectors . Studies are currently in progress in order to identify novel insulators that have the ability to prevent genotoxic effects of integrating gene delivery systems .
A major inadequacy of self-inactivating retroviridae-derived vectors consists of an increased polyadenylation (polyA) signal read-through in the 3′-LTR (Figure 6) . Leaky transcriptional termination signals may result in the activation of silent cellular oncogenes in transduced cells (Figure 6) . Furthermore, the polyA read-through in packaging cell lines might cause the uptake of cellular oncogenes into the genome of retroviridae-based vectors, which, in turn, may transmit it to transduced cell populations . This phenomenon occurred in so-called acute transforming retroviruses [119–122]. The deletion of the U3 region from the 3′-LTR enhances the probability of polyA read-through in self-inactivating retroviridae-based vectors [123, 124]. In fact, the U3 region of the LTR contains both transcription termination motifs and enhancer-promoter elements [123, 124]. In this respect, a study demonstrated that the incorporation of seven SV40-derived upstream polyA enhancer elements in the residual U3 region improved transcription termination efficiency in self-inactivating retroviridae-based vectors . This study reported also a 3-fold enhancement in viral vector titers, along with increased and stable transgene expression levels in transduced cell populations .
Insertional mutagenesis-induced malignancies were also observed in animal models for AAV-mediated gene transfer . Wild-type AAV integrates specifically into a safe site of the human genome . This safe integration site is termed AAV1 and is located in the q arm of the chromosome 19, between q13-3 and qter [3, 12]. The wild-type AAV-specific integration into AAV1 is mediated by the viral factors Rep 68 and Rep 78 [3, 12]. However, most of the wild-type AAV genome must be removed in order to engineer AAV-derived vector systems, because of the limited capacity of AAV-based vectors in accommodating transgenes [3, 4, 12]. For this reason, AAV-derived vectors do not have Rep 68 and Rep 78 proteins and, therefore, integrate randomly within the cellular genome [3, 4, 12]. The field of vector design is attempting to restore AAV1-specific integration via coexpression in transduced cell populations of AAV-based vectors along with Rep 68 and Rep 78 proteins .
Another strategy to circumvent the issue of insertional mutagenesis consists of producing episomal gene delivery models with enhanced duration of transgene expression. On one hand, episomal vector systems do not alter the transduced cell genome, as they do not integrate into the chromosomal DNA . On the other hand, nonintegrating gene delivery models only allow for a transient expression of the transgene . On these grounds, the field of vector design is working on the development of novel systems for the stabilization of transgene expression after the episomal vector-mediated gene transfer into target cells. To this end, gene therapists placed a particular emphasis on the development on episomal lentiviral vectors [125–135], because of their ability to transduce proficiently an ample variety of mammalian cells and regardless of their cell cycle phase . Several studies showed that episomal lentiviral-based vectors maintained high transduction efficacy and wide cell tropism [125, 128–131]. Studies on mouse models focused on in vivo gene delivery of episomal lentiviral vectors in the central nervous system , stem cells of the hematopoietic compartment , muscles , and ocular tissues . Episomal lentiviral vector systems can be produced via inactivation of the viral integrase and mutation of the integrase attachment sites (att) that are present in the U3 region of the 5′- and 3′-LTRs . The viral integrase is inactivated by site directed mutagenesis of the chromosome-binding moiety, proviral DNA binding domain and catalytic site [125, 130]. These point mutations do not affect at all the ability of the viral integrase to transport the pre-integration complex through the nuclear membrane [125, 130]. Negligible levels of residual integration of episomal lentiviral vectors were reported in some transduced cell populations . Nevertheless, such a residual integration activity was related to background recombination events, rather than the mutated viral integrase . Episomal lentiviral vector systems allow for a better long-term transgene expression in nondividing cells than in dividing cells, as mitosis may cause the dilution of the episomal vector genome in progeny cells [125, 128, 131]. Remarkably, the inclusion of scaffold/matrix attachment regions (S/MAR) may increase the duration of transgene expression of episomal lentiviral vector systems within transduced cell populations [136, 137]. This approach may also be utilized to enhance the episomal permanence of non-viral gene-delivery models in transfected human cells [137, 138]. In fact, promising results were reported in human hematopoietic progenitor cells . However, the efficacy of S/MAR in increasing episomal permanence was cell-type-dependent . Further research is necessary to improve the preclinical applications of nonintegrating gene delivery models.
Meganucleases and artificially engineered zinc-finger proteins are attracting a great deal of interest in the field of gene transfer technology, as they hold the potential of conducting either site-specific rectification of defective genes, or inclusion of genetic elements into selected loci within the genome of transfected cells [139–143]. ZFPs have the ability to identify several varieties of DNA motifs [139–142]. The C2H2-type ZFPs are by far the most common DNA-binding moieties of transcription factors . Amazingly, such DNA-binding domains constitute about 2% of the entire human genome [139–144]. Novel C2H2-type ZFPs can be designed for the binding of specific DNA sequences. This strategy relies on the variation of C2H2-type ZFPs that are present in the Sp1 transcription factor . A similar tactic was utilized for the production of recombinant meganucleases or homing endonucleases [143, 145–148].
Meganucleases consist of sequence-specific endonucleases that identify relatively large DNA binding motifs in living cells . Such DNA binding motifs are in the range of 14 base pairs (bp) or more . The production of genetically modified meganucleases might allow for the design of site-specific gene delivery into the human genome [145–148]. For example, the I-CreI-derived meganuclease DNA binding domain was adjusted to recognize specifically new designed genetic sequences [145–147]. The artificial I-CreI-derived meganuclease was utilized to target the human xeroderma pigmentosum group C (XPC) gene, whose genetic mutations may be responsible for the pathogenesis of Xeroderma Pigmentosa disease , which is a rare autosomal recessive genetic illness causing both hypersensitivity to ultraviolet light and high incidence of skin cancer . Two independent reports showed an efficient I-CreI-derived meganuclease-mediated rectification of a flawed chromosomal locus in various mammalian cell lines [146, 148]. Interestingly, the employment of genetically engineered meganucleases was not associated with noticeable levels of genotoxicity in mammalian cell lines [143, 148]. Other studies are currently underway for the characterization of I-SceI [150–152] and I-DmoI meganucleases .
The possible onset of insertional mutagenesis-induced malignancies in patients constitutes a serious obstruction to the establishment of gene therapy programs in the clinical setting. Undeniably, gene transfer technology holds enormous therapeutic potential for the treatment of a wide variety of pathological conditions, such as cancer, cardiovascular disorders, genetic diseases, diabetes, neurodegenerative illnesses, and infectious maladies [3, 4]. This is the reason that motivates a keen interest for gene therapy. Moreover, the merging between gene transfer technology and stem cell research may have important implications in the area of regenerative medicine.
The field of vector design is currently tackling the critical issue of insertional mutagenesis by adopting three main strategies. The first approach is based on the production of integrating gene delivery models that can only express the transgene and do not interact with the human genome, in order to avoid the activation of cellular oncogenes. In this respect, enhancer-less self-inactivating retroviridae-derived vectors have attracted a lot of interest and seem very promising. The second approach depends on the engineering of episomal vector systems, which were genetically modified to allow for a more stable and long-lasting transgene expression in transduced cell populations. The third approach utilizes genetically modified meganucleases and artificially engineered zinc-finger proteins to carry out either site-specific correction of faulty genes or inclusion of genetic factors into specific and safe loci of the genome of transfected cells. These strategies require major efforts from gene therapists. However, the solution of the insertional mutagenesis issue may bring a substantial contribution to a more successful application of gene therapy programs in various sectors of experimental medicine.
This paper is dedicated to the memory of Martino Massi (fra’ Martino) and Marcello Ripamonti (fra’ Marcellino). The author wishes to thank Dr. Nurit Pilpel for her helpful comments on the paper.
- M. L. Edelstein, M. R. Abedi, J. Wixon, and R. M. Edelstein, “Gene therapy clinical trials worldwide 1989 2004—an overview,” Journal of Gene Medicine, vol. 6, no. 6, pp. 597–602, 2004.
- M. L. Edelstein, M. R. Abedi, and J. Wixon, “Gene therapy clinical trials worldwide to 2007—an update,” Journal of Gene Medicine, vol. 9, no. 10, pp. 833–842, 2007.
- G. Romano, “Gene transfer in experimental medicine,” Drug News and Perspectives, vol. 16, no. 5, pp. 267–276, 2003.
- G. Romano, P. Micheli, C. Pacilio, and A. Giordano, “Latest developments in gene transfer technology: achievements, perspectives, and controversies over therapeutic applications,” Stem Cells, vol. 18, no. 1, pp. 19–39, 2000.
- B. J. Uttenthal I. Chua, E. C. Morris, and H. J. Strauss, “Challenges in T cell receptor gene therapy,” The Journal of Gene Medicine, vol. 14, no. 6, pp. 386–399, 2012.
- R. A. Willemsen, R. Debets, P. Chames, and R. L. H. Bolhuis, “Genetic engineering of T cell specificity for immunotherapy of cancer,” Human Immunology, vol. 64, no. 1, pp. 56–68, 2003.
- S. Thomas, H. J. Stauss, and E. C. Morris, “Molecular immunology lessons from therapeutic T-cell receptor gene transfer,” Immunology, vol. 129, no. 2, pp. 170–177, 2010.
- S. A. Rosenberg, P. Aebersold, K. Cornetta et al., “Gene transfer into humans—immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction,” New England Journal of Medicine, vol. 323, no. 9, pp. 570–578, 1990.
- R. M. Blaese, K. W. Culver, A. D. Miller et al., “T lymphocyte-directed gene therapy for ADA- SCID: initial trial results after 4 years,” Science, vol. 270, no. 5235, pp. 475–480, 1995.
- L. M. Muul, L. M. Tuschong, S. L. Soenen et al., “Persistence and expression of the adenosine deaminase gene for 12 years and immune reaction to gene transfer components: long-term results of the first clinical gene therapy trial,” Blood, vol. 101, no. 7, pp. 2563–2569, 2003.
- G. Romano, “Current development of lentiviral-mediated gene transfer,” Drug News and Perspectives, vol. 18, no. 2, pp. 128–134, 2005.
- G. Romano, “Current development of adeno-associated viral vectors,” Drug News and Perspectives, vol. 18, no. 5, pp. 311–316, 2005.
- G. Romano, “The controversial role of adenoviral-derived vectors in gene therapy programs: Where do we stand?” Drug News and Perspectives, vol. 19, no. 2, pp. 99–106, 2006.
- G. Romano, “Current development of nonviral-mediated gene transfer,” Drug News and Perspectives, vol. 20, no. 4, pp. 227–231, 2007.
- G. Romano, P. P. Claudio, T. Tonini, and A. Giordano, “Human immunodeficiency virus type 1 (HIV-1) derived vectors: safety considerations and controversy over therapeutic applications,” European Journal of Dermatology, vol. 13, no. 5, pp. 424–429, 2003.
- B. L. Levine, L. M. Humeau, J. Boyer et al., “Gene transfer in humans using a conditionally replicating lentiviral vector,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 46, pp. 17372–17377, 2006.
- G. Romano, “Latest advances in gene transfer technology: a summary on the fourteen annual meeting of the American Society of Gene and Cell Therapy,” Drugs of the Future, vol. 36, no. 7, pp. 535–541, 2011.
- M. Brenner, “Gene transfer by adenovectors,” Blood, vol. 94, no. 12, pp. 3965–3967, 1999.
- G. Romano, “The standpoint of stem cell research,” Drug News and Perspectives, vol. 21, no. 7, pp. 408–412, 2008.
- G. Romano, “Artificial reprogramming of human somatic cells to generate pluripotent stem cells: a possible alternative to the controversial use of human embryonic stem cells,” Drug News & Perspectives, vol. 21, no. 8, pp. 440–445, 2008.
- I. A. Muchkaeva, E. B. Dashinimaev, V. V. Terskikh, Y. V. Sukhanov, and A. V. Vasiliev, “Molecular mechanisms of induced pluripotency,” ActaNaturae, vol. 4, no. 1, pp. 12–22, 2012.
- C. Wu and C. E. Dunbar, “Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity,” Frontiers of Medicine in China, vol. 5, no. 4, pp. 356–371, 2011.
- X. Ren, C. G. T. Tahimic, M. Katoh, A. Kurimasa, T. Inoue, and M. Oshimura, “Human artificial chromosome vectors meet stem cells: new prospects for gene delivery,” Stem Cell Reviews, vol. 2, no. 1, pp. 43–50, 2006.
- P. Asuri, M. A. Bartel, T. Vazin, J.-H. Jang, T. B. Wong, and D. V. Schaffer, “Directed evolution of adeno-associated virus for enhanced gene delivery and gene targeting in human pluripotent stem cells,” Molecular Therapy, vol. 20, no. 2, pp. 329–338, 2012.
- Y. Strulovici, P. L. Leopold, T. P. O'Connor, R. G. Pergolizzi, and R. G. Crystal, “Human embryonic stem cells and gene therapy,” Molecular Therapy, vol. 15, no. 5, pp. 850–866, 2007.
- V. Sueblinvong, B. T. Suratt, and D. J. Weiss, “Novel therapies for the treatment of cystic fibrosis: new developments in gene and stem cell therapy,” Clinics in Chest Medicine, vol. 28, no. 2, pp. 361–379, 2007.
- U. R. Goessler, K. Riedel, K. Hörmann, and F. Riedel, “Perspectives of gene therapy in stem cell tissue engineering,” Cells Tissues Organs, vol. 183, no. 4, pp. 169–179, 2006.
- N. Kimelman, G. Pelled, G. A. Helm, J. Huard, E. M. Schwarz, and D. Gazit, “Review: gene- and stem cell-based therapeutics for bone regeneration and repair,” Tissue Engineering, vol. 13, no. 6, pp. 1135–1150, 2007.
- J. A. Korecka, J. Verhaagen, and E. M. Hol, “Cell-replacement and gene-therapy strategies for Parkinson's and Alzheimer's disease,” Regenerative Medicine, vol. 2, no. 4, pp. 425–446, 2007.
- K. Takahashi, K. Tanabe, M. Ohnuki et al., “Induction of pluripotent stem cells from adult human fibroblasts by defined factors,” Cell, vol. 131, no. 5, pp. 861–872, 2007.
- J. Yu, M. A. Vodyanik, K. Smuga-Otto et al., “Induced pluripotent stem cell lines derived from human somatic cells,” Science, vol. 318, no. 5858, pp. 1917–1920, 2007.
- G. Romano, “Latest advances in iPS cell technology: toward the delineation of the genetic and epigenetic codes for pluripotency and development of a novel system for the study of human maladies and drug screening,” Drugs of the Future, vol. 37, no. 6, pp. 431–435, 2012.
- K. H. Narsinh and J. C. Wu, “Gene correction in human embryonic and induced pluripotent stem cells: promises and challenges ahead,” Molecular Therapy, vol. 18, no. 6, pp. 1061–1063, 2010.
- C. Baum, “What are the consequences of the fourth case?” Molecular Therapy, vol. 15, no. 8, pp. 1401–1402, 2007.
- C. Baum, “Gene therapy for SCID-X1: focus on clinical data,” Molecular Therapy, vol. 19, no. 12, pp. 2103–2104, 2011.
- M. Cavazzana-Calvo and A. Fischer, “Gene therapy for severe combined immunodeficiency: Are we there yet?” Journal of Clinical Investigation, vol. 117, no. 6, pp. 1456–1465, 2007.
- S. Hacein-Bey-Abina, C. von Kalle, M. Schmidt et al., “LMO2-Associated clonal T cell proliferation in two patients after gene therapy for SCID-X1,” Science, vol. 302, no. 5644, pp. 415–419, 2003.
- S. Hacein-Bey-Abina, A. Garrigue, G. P. Wang et al., “Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1,” Journal of Clinical Investigation, vol. 118, no. 9, pp. 3132–3142, 2008.
- S. Hacein-Bey-Abina, C. von Kalle, M. Schmidt et al., “A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency,” New England Journal of Medicine, vol. 348, no. 3, pp. 255–256, 2003.
- P. Noguchi, “Risks and benefits of gene therapy,” New England Journal of Medicine, vol. 348, no. 3, pp. 193–194, 2003.
- J. Chinen and J. M. Puck, “Successes and risks of gene therapy in primary immunodeficiencies,” Journal of Allergy and Clinical Immunology, vol. 113, no. 4, pp. 595–604, 2004.
- S. J. Howe, M. R. Mansour, K. Schwarzwaelder et al., “Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients,” Journal of Clinical Investigation, vol. 118, no. 9, pp. 3143–3150, 2008.
- K. Pike-Overzet, M. van der Burg, G. Wagemaker, J. J. M. van Dongen, and F. J. T. Staal, “New insights and unresolved issues regarding insertional mutagenesis in X-linked SCID gene therapy,” Molecular Therapy, vol. 15, no. 11, pp. 1910–1916, 2007.
- C. Baum, “Insertional mutagenesis in gene therapy and stem cell biology,” Current Opinion in Hematology, vol. 14, no. 4, pp. 337–342, 2007.
- M. Cavazzana-Calvo, S. Hacein-Bey, G. de Saint Basile et al., “Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease,” Science, vol. 288, no. 5466, pp. 669–672, 2000.
- M. P. McCormack and T. H. Rabbitts, “Activation of the T-cell oncogene LMO2 after gene therapy for X-linked Severe combined immunodeficiency,” New England Journal of Medicine, vol. 350, no. 9, pp. 913–922, 2004.
- H. B. Gaspar, K. L. Parsley, S. Howe et al., “Gene therapy of X-linked severe combined immunodeficiency by use of a pseudotyped gammaretroviral vector,” The Lancet, vol. 364, no. 9452, pp. 2181–2187, 2004.
- S. L. Ginn, J. A. Curtin, B. Kramer et al., “Treatment of an infant with X-linked severe combined immunodeficiency (SCID-X1) by gene therapy in Australia,” Medical Journal of Australia, vol. 182, no. 9, pp. 458–463, 2005.
- J. Chinen, J. Davis, S. S. de Ravin et al., “Gene therapy improves immune function in preadolescents with X-linked severe combined immunodeficiency,” Blood, vol. 110, no. 1, pp. 67–73, 2007.
- K. Schwarzwaelder, S. J. Howe, M. Schmidt et al., “Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo,” Journal of Clinical Investigation, vol. 117, no. 8, pp. 2241–2249, 2007.
- R. Quinonez and R. E. Sutton, “Lentiviral vectors for gene delivery into cells,” DNA and Cell Biology, vol. 21, no. 12, pp. 937–951, 2002.
- K. El Omari, S. J. Hoosdally, K. Tuladhar et al., “Structure of the leukemia oncogene LMO2: implications for the assembly of a hematopoietic transcription factor complex,” Blood, vol. 117, no. 7, pp. 2146–2156, 2011.
- D. J. Curtis and M. McCormack, “The molecular basis of Lmo2-induced T-cell acute lymphoblastic leukemia,” Clinical Cancer Research, vol. 16, no. 23, pp. 5618–5623, 2010.
- I. Homminga, M. J. Vuerhard, A. W. Langerak, J. Buijs-Gladdines, R. Pieters, and J. P. P. Meijerink, “Characterization of a pediatric T-cell acute lymphoblastic leukemia patient with simultaneous LYL1 and LMO2 rearrangements,” Haematologica, vol. 97, no. 2, pp. 258–261, 2012.
- R. Malumbres, V. Fresquet, J. Roman-Gomez et al., “LMO2 expression reflects the different stages of blast maturation and genetic features in B-cell acute lymphoblastic leukemia and predicts clinical outcome,” Haematologica, vol. 96, no. 7, pp. 980–986, 2011.
- U. P. Davé, N. A. Jenkins, and N. G. Copeland, “Gene therapy insertional mutagenesis insights,” Science, vol. 303, no. 5656, p. 333, 2004.
- N. B. Woods, V. Bottero, M. Schmidt, C. von Kalle, and I. M. Verma, “Gene therapy: therapeutic gene causing lymphoma,” Nature, vol. 440, no. 7088, p. 1123, 2006.
- U. Modlich, A. Schambach, M. H. Brugman et al., “Leukemia induction after a single retroviral vector insertion in Evi1 or Prdm16,” Leukemia, vol. 22, no. 8, pp. 1519–1528, 2008.
- K. Pike-Overzet, D. de Ridder, F. Weerkamp et al., “Gene therapy: Is IL2RG oncogenic in T-cell development?” Nature, vol. 443, no. 7109, p. E5, 2006.
- A. J. Thrasher, H. B. Gaspar, C. Baum et al., “Gene therapy: X-SCID transgene leukaemogenicity,” Nature, vol. 443, no. 7109, pp. E5–E6, 2006.
- A. Fucito, C. Lucchetti, A. Giordano, and G. Romano, “Genetic and epigenetic alterations in breast cancer: What are the perspectives for clinical practice?” International Journal of Biochemistry and Cell Biology, vol. 40, no. 4, pp. 565–575, 2008.
- A. Giordano, A. Fucito, G. Romano, and I. R. Marino, “Carcinogenesis and environment: the cancer stem cell hypothesis and implications for the development of novel therapeutics and diagnostics,” Frontiers in Bioscience, vol. 12, pp. 3475–3482, 2007.
- A. L. Welm, S. Kim, B. E. Welm, and J. M. Bishop, “MET and MYC cooperate in mammary tumorigenesis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 12, pp. 4324–4329, 2005.
- D. D. Gan, M. Macaluso, C. Cinti, K. Khalili, and A. Giordano, “How does a normal human cell become a cancer cell?” Journal of Experimental and Clinical Cancer Research, vol. 22, no. 4, pp. 509–516, 2003.
- M. J. Berardi and V. R. Fantin, “Survival of the fittest: metabolic adaptations in cancer,” Current Opinion in Genetics and Development, vol. 21, no. 1, pp. 59–66, 2011.
- G. Romano, “The complex biology of the receptor for the insulin-like growth factor-1,” Drug News and Perspectives, vol. 16, no. 8, pp. 525–531, 2003.
- D. F. Merlo, R. Filiberti, M. Kobernus et al., “Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence,” Environmental Health, vol. 11, supplement 1, Article ID S9, 2012.
- A. Sun, L. Bagella, S. Tutton, G. Romano, and A. Giordano, “From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway,” Journal of Cellular Biochemistry, vol. 102, no. 6, pp. 1400–1404, 2007.
- A. Giordano, A. Rossi, G. Romano, and L. Bagella, “Tumor suppressor pRb2/p130 gene and its derived product Spa310 spacer domain as perspective candidates for cancer therapy,” Journal of Cellular Physiology, vol. 213, no. 2, pp. 403–406, 2007.
- M. H. Sherman, M. Downes, and R. M. Evans, “Nuclear receptors as modulators of the tumor microenvironment,” Cancer Prevention Research, vol. 5, no. 1, pp. 3–10, 2012.
- C. Florean, M. Schnekenburger, C. Grandjenette, M. Dicato, and M. Diederich, “Epigenomics of leukemia: from mechanisms to therapeutic applications,” Epigenomics, vol. 3, no. 5, pp. 581–609, 2011.
- J. Füllgrabe, E. Kavanagh, and B. Joseph, “Histone onco-modifications,” Oncogene, vol. 30, no. 31, pp. 3391–3403, 2011.
- R. Gabriel, R. Eckenberg, A. Paruzynski et al., “Comprehensive genomic access to vector integration in clinical gene therapy,” Nature Medicine, vol. 15, no. 12, pp. 1431–1436, 2009.
- J.-Y. Métais and C. E. Dunbar, “The MDS1-EVI1 gene complex as a retrovirus integration site: impact on behavior of hematopoietic cells and implications for gene therapy,” Molecular Therapy, vol. 16, no. 3, pp. 439–449, 2008.
- A. Paruzynski, A. Arens, R. Gabriel et al., “Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing,” Nature Protocols, vol. 5, no. 8, pp. 1379–1395, 2010.
- G. Romano, “Recent advances in gene therapy programs: highlights from the 13th Annual Meeting of the American Society of Gene & Cell Therapy,” Drugs of the Future, vol. 35, no. 8, pp. 687–694, 2010.
- A. Deichmann, M. H. Brugman, C. C. Bartholomae et al., “Insertion sites in engrafted cells cluster within a limited repertoire of genomic areas after gammaretroviral vector gene therapy,” Molecular Therapy, vol. 19, no. 11, pp. 2031–2039, 2011.
- A. Paruzynski, H. Glimm, M. Schmidt, and C. von Kalle, “Analysis of the clonal repertoire of gene-corrected cells in gene therapy,” Methods in Enzymology, vol. 507, pp. 59–87, 2012.
- A. Galy and A. J. Thrasher, “Gene therapy for the Wiskott-Aldrich syndrome,” Current Opinion in Allergy and Clinical Immunology, vol. 11, no. 6, pp. 545–550, 2011.
- O. S. Kustikova, U. Modlich, and B. Fehse, “Retroviral insertion site analysis in dominant haematopoietic clones,” Methods in Molecular Biology, vol. 506, pp. 373–390, 2009.
- A. Deichmann, S. Hacein-Bey-Abina, M. Schmidt et al., “Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID-X1 gene therapy,” Journal of Clinical Investigation, vol. 117, no. 8, pp. 2225–2232, 2007.
- U. Modlich, O. S. Kustikova, M. Schmidt et al., “Leukemias following retroviral transfer of multidrug resistance 1 (MDR1) are driven by combinatorial insertional mutagenesis,” Blood, vol. 105, no. 11, pp. 4235–4246, 2005.
- O. Kustikova, B. Fehse, U. Modlich et al., “Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking,” Science, vol. 308, no. 5725, pp. 1171–1174, 2005.
- T. Maetzig, M. H. Brugman, S. Bartels et al., “Polyclonal fluctuation of lentiviral vector-transduced and expanded murine hematopoietic stem cells,” Blood, vol. 117, no. 11, pp. 3053–3064, 2011.
- R. S. Mitchell, B. F. Beitzel, A. R. W. Schroder et al., “Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences,” PLoS Biology, vol. 2, no. 8, p. E234, 2004.
- M. K. Lewinski, M. Yamashita, M. Emerman et al., “Retroviral DNA integration: viral and cellular determinants of target-site selection,” PLoS Pathogens, vol. 2, no. 6, p. E60, 2006.
- A. Ciuffi, R. S. Mitchell, C. Hoffmann et al., “Integration site selection by HIV-based vectors in dividing and growth-arrested IMR-90 lung fibroblasts,” Molecular Therapy, vol. 13, no. 2, pp. 366–373, 2006.
- A. R. W. Schröder, P. Shinn, H. Chen, C. Berry, J. R. Ecker, and F. Bushman, “HIV-1 integration in the human genome favors active genes and local hotspots,” Cell, vol. 110, no. 4, pp. 521–529, 2002.
- S. D. Barr, A. Ciuffi, J. Leipzig, P. Shinn, J. R. Ecker, and F. D. Bushman, “HIV integration site selection: targeting in macrophages and the effects of different routes of viral entry,” Molecular Therapy, vol. 14, no. 2, pp. 218–225, 2006.
- A. C. Bester, M. Schwartz, M. Schmidt et al., “Fragile sites are preferential targets for integrations of MLV vectors in gene therapy,” Gene Therapy, vol. 13, no. 13, pp. 1057–1059, 2006.
- L. F. Maxfield, C. D. Fraize, and J. M. Coffin, “Relationship between retroviral DNA-integration-site selection and host cell transcription,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 5, pp. 1436–1441, 2005.
- G. Romano, “The standpoint of gene therapy programs,” Drug News and Perspectives, vol. 20, no. 5, pp. 335–343, 2007.
- B. Moldt, S. R. Yant, P. R. Andersen, M. A. Kay, and J. G. Mikkelsen, “Cis-acting gene regulatory activities in the terminal regions of Sleeping Beauty DNA transposon-based vectors,” Human Gene Therapy, vol. 18, no. 12, pp. 1193–1204, 2007.
- J. U. Appelt, F. A. Giordano, M. Ecker et al., “QuickMap: a public tool for large-scale gene therapy vector insertion site mapping and analysis,” Gene Therapy, vol. 16, no. 7, pp. 885–893, 2009.
- N. C. Popescu, “Genetic alterations in cancer as a result of breakage at fragile sites,” Cancer Letters, vol. 192, no. 1, pp. 1–17, 2003.
- D. G. Miller, L. M. Petek, and D. W. Russell, “Adeno-associated virus vectors integrate at chromosome breakage sites,” Nature Genetics, vol. 36, no. 7, pp. 767–773, 2004.
- C. H. Freudenreich, “Chromosome fragility: molecular mechanisms and cellular consequences,” Frontiers in Bioscience, vol. 12, pp. 4911–4924, 2007.
- H. M. Padilla-Nash, K. Heselmeyer-Haddad, D. Wangsa et al., “Jumping translocations are common in solid tumor cell lines and result in recurrent fusions of whole chromosome arms,” Genes Chromosomes and Cancer, vol. 30, no. 4, pp. 349–363, 2001.
- D. I. Smith, S. McAvoy, Y. Zhu, and D. S. Perez, “Large common fragile site genes and cancer,” Seminars in Cancer Biology, vol. 17, no. 1, pp. 31–41, 2007.
- L. W. Dillon, A. A. Burrow, and Y. H. Wang, “DNA instability at chromosomal fragile sites in cancer,” Current Genomics, vol. 11, no. 5, pp. 326–337, 2010.
- D. Bystricka, I. Sarova, Z. Zemanova et al., “Recurrent chromosomal breakpoints in patients with myelodysplastic syndromes and complex karyotype versus fragile sites,” Leukemia Research, vol. 36, no. 6, pp. e125–e127, 2012.
- A. Fungtammasan, E. Walsh, F. Chiaromonte, K. A. Eckert, and K. D. Makova, “A genome-wide analysis of common fragile sites: What features determine chromosomal instability in the human genome?” Genome Research, vol. 22, no. 6, pp. 993–1005, 2012.
- K. L. Dall, C. G. Scarpini, I. Roberts et al., “Characterization of naturally occurring HPV16 integration sites isolated from cervical keratinocytes under noncompetitive conditions,” Cancer Research, vol. 68, no. 20, pp. 8249–8259, 2008.
- G. Romano, “Viral oncology and development of preventive vaccines,” Drugs of the Future, vol. 32, no. 4, pp. 367–373, 2007.
- M. Schmitz, C. Driesch, K. Beer-Grondke, L. Jansen, I. B. Runnebaum, and M. Dürst, “Loss of gene function as a consequence of human papillomavirus DNA integration,” International Journal of Cancer, vol. 131, no. 5, pp. E593–E602, 2012.
- L. Nambaru, B. Meenakumari, R. Swaminathan, and T. Rajkumar, “Prognostic significance of HPV physical status and integration sites in cervical cancer,” Asian Pacific Journal of Cancer Prevention, vol. 10, no. 3, pp. 355–360, 2009.
- W. J. Luo, T. Takakuwa, M. F. Ham et al., “Epstein-Barr virus is integrated between REL and BCL-11A in American Burkitt lymphoma cell line (NAB-2),” Laboratory Investigation, vol. 84, no. 9, pp. 1193–1199, 2004.
- A. Jox, C. Rohen, G. Belge et al., “Integration of Epstein-Barr virus in Burkitt's lymphoma cells leads to a region of enhanced chromosome instability,” Annals of Oncology, vol. 8, supplement 2, pp. S131–S135, 1997.
- M. A. Feitelson and J. Lee, “Hepatitis B virus integration, fragile sites, and hepatocarcinogenesis,” Cancer Letters, vol. 252, no. 2, pp. 157–170, 2007.
- F. Zhang, S. I. Thornhill, S. J. Howe et al., “Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells,” Blood, vol. 110, no. 5, pp. 1448–1457, 2007.
- E. Almarza, F. Zhang, G. Santilli et al., “Correction of SCID-X1 using an enhancerless vav promoter,” Human Gene Therapy, vol. 22, no. 3, pp. 263–270, 2011.
- S. Knight, F. Zhang, U. Mueller-Kuller et al., “Safer, silencing-resistant lentiviral vectors: optimization of the ubiquitous chromatin-opening element through elimination of aberrant splicing,” Journal of Virology, vol. 86, no. 17, pp. 9088–9095, 2012.
- Á. González-Murillo, M. L. Lozano, L. Álvarez et al., “Development of lentiviral vectors with optimized transcriptional activity for the gene therapy of patients with fanconi anemia,” Human Gene Therapy, vol. 21, no. 5, pp. 623–630, 2010.
- J. Soulier, “Fanconi anemia,” American Society of Hematology Education Program, pp. 492–497, 2011.
- S. Zhou, D. Mody, S. S. DeRavin et al., “A self-inactivating lentiviral vector for SCID-X1 gene therapy that does not activate LMO2 expression in human T cells,” Blood, vol. 116, no. 6, pp. 900–908, 2010.
- N. Uchida, K. N. Washington, C. J. Lap, M. M. Hsieh, and J. F. Tisdale, “Chicken HS4 insulators have minimal barrier function among progeny of human hematopoietic cells transduced with an HIV1-based lentiviral vector,” Molecular Therapy, vol. 19, no. 1, pp. 133–139, 2011.
- S. Rivella, J. A. Callegari, C. May, C. W. Tan, and M. Sadelain, “The cHS4 insulator increases the probability of retroviral expression at random chromosomal integration sites,” Journal of Virology, vol. 74, no. 10, pp. 4679–4687, 2000.
- D. W. Emery, “The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors,” Human Gene Therapy, vol. 22, no. 6, pp. 761–774, 2011.
- A. Schambach, M. Galla, T. Maetzig, R. Loew, and C. Baum, “Improving transcriptional termination of self-inactivating gamma-retroviral and lentiviral vectors,” Molecular Therapy, vol. 15, no. 6, pp. 1167–1173, 2007.
- M. P. Felder, A. Eychene, D. Laugier, M. Marx, P. Dezelee, and G. Calothy, “Steps and mechanisms of oncogene transduction by retroviruses,” Folia Biologica, vol. 40, no. 5, pp. 225–235, 1994.
- C. Stocking, R. Kollek, U. Bergholz, and W. Ostertag, “Long terminal repeat sequences impart hematopoietic transformation properties to the myeloproliferative sarcoma virus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 82, no. 17, pp. 5746–5750, 1985.
- J. S. Lipsick and D. M. Wang, “Transformation by v-Myb,” Oncogene, vol. 18, no. 19, pp. 3047–3055, 1999.
- A. K. Zaiss, S. Son, and L. J. Chang, “RNA 3′ readthrough of oncoretrovirus and lentivirus: implications for vector safety and efficacy,” Journal of Virology, vol. 76, no. 14, pp. 7209–7219, 2002.
- G. M. Gilmartin, E. S. Fleming, and J. Oetjen, “Activation of HIV-1 pre-mRNA 3' processing in vitro requires both an upstream element and TAR,” EMBO Journal, vol. 11, no. 12, pp. 4419–4428, 1992.
- N. J. Philpott and A. J. Thrasher, “Use of nonintegrating lentiviral vectors for gene therapy,” Human Gene Therapy, vol. 18, no. 6, pp. 483–489, 2007.
- B. Hu, H. Yang, B. Dai, A. Tai, and P. Wang, “Nonintegrating lentiviral vectors can effectively deliver ovalbumin antigen for induction of antitumor immunity,” Human Gene Therapy, vol. 20, no. 12, pp. 1652–1664, 2009.
- D. R. M. Negri, R. Bona, Z. Michelini et al., “Transduction of human antigen-presenting cells with integrase-defective lentiviral vector enables functional expansion of primed antigen-specific CD8+ T cells,” Human Gene Therapy, vol. 21, no. 8, pp. 1029–1035, 2010.
- S. Philippe, C. Sarkis, M. Barkats et al., “Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 47, pp. 17684–17689, 2006.
- J. Vargas, G. L. Gusella, V. Najfeld, M. E. Klotman, and A. Cara, “Novel integrase-defective lentiviral episomal vectors for gene transfer,” Human Gene Therapy, vol. 15, no. 4, pp. 361–372, 2004.
- A. V. Terskikh, M. A. Ershler, N. J. Drize, I. N. Nifontova, and J. L. Chertkov, “Long-term persistence of a nonintegrated lentiviral vector in mouse hematopoietic stem cells,” Experimental Hematology, vol. 33, no. 8, pp. 873–882, 2005.
- L. Apolonia, S. N. Waddington, C. Fernandes et al., “Stable gene transfer to muscle using non-integrating lentiviral vectors,” Molecular Therapy, vol. 15, no. 11, pp. 1947–1954, 2007.
- K. Karwacz, S. Mukherjee, L. Apolonia et al., “Nonintegrating lentivector vaccines stimulate prolonged T-cell and antibody responses and are effective in tumor therapy,” Journal of Virology, vol. 83, no. 7, pp. 3094–3103, 2009.
- S. J. Nightingale, R. P. Hollis, K. A. Pepper et al., “Transient gene expression by nonintegrating lentiviral vectors,” Molecular Therapy, vol. 13, no. 6, pp. 1121–1132, 2006.
- B. Kantor, M. Bayer, H. Ma et al., “Notable reduction in illegitimate integration mediated by a PPT-deleted, nonintegrating lentiviral vector,” Molecular Therapy, vol. 19, no. 3, pp. 547–556, 2011.
- R. J. Yáñez-Muñoz, K. S. Balaggan, A. MacNeil et al., “Effective gene therapy with nonintegrating lentiviral vectors,” Nature Medicine, vol. 12, no. 3, pp. 348–353, 2006.
- A. Baiker, C. Maercker, C. Piechaczek et al., “Mitotic stability of an episomal vector containing a human scaffold/matrix-attached region is provided by association with nuclear matrix,” Nature Cell Biology, vol. 2, no. 3, pp. 182–184, 2000.
- M. M. P. Lufino, R. Manservigi, and R. Wade-Martins, “An S/MAR-based infectious episomal genomic DNA expression vector provides long-term regulated functional complementation of LDLR deficiency,” Nucleic Acids Research, vol. 35, no. 15, article e98, 2007.
- E. P. Papapetrou, P. G. Ziros, I. D. Micheva, N. C. Zoumbos, and A. Athanassiadou, “Gene transfer into human hematopoietic progenitor cells with an episomal vector carrying an S/MAR element,” Gene Therapy, vol. 13, no. 1, pp. 40–51, 2006.
- M. Dhanasekaran, S. Negi, and Y. Sugiura, “Designer zinc finger proteins: tools for creating artificial DNA-binding functional proteins,” Accounts of Chemical Research, vol. 39, no. 1, pp. 45–52, 2006.
- C. L. Dent, G. Lau, E. A. Drake, A. Yoon, C. C. Case, and P. D. Gregory, “Regulation of endogenous gene expression using small molecule-controlled engineered zinc-finger protein transcription factors,” Gene Therapy, vol. 14, no. 18, pp. 1362–1369, 2007.
- S. Dion, M. V. Demattéi, and S. Renault, “Zinc finger proteins: tools for site-specific correction or modification of the genome,” Medecine/Sciences, vol. 23, no. 10, pp. 834–839, 2007.
- P. J. Verschure, A. E. Visser, and M. G. Rots, “Step out of the groove: epigenetic gene control systems and engineered transcription factors,” Advances in Genetics, vol. 56, pp. 163–204, 2006.
- F. Pâques and P. Duchateau, “Meganucleases and DNA double-strand break-induced recombination: perspectives for gene therapy,” Current Gene Therapy, vol. 7, no. 1, pp. 49–66, 2007.
- S. Iuchi, “Three classes of C2H2 zinc finger proteins,” Cellular and Molecular Life Sciences, vol. 58, no. 4, pp. 625–635, 2001.
- J. Smith, S. Grizot, S. Arnould et al., “A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences,” Nucleic Acids Research, vol. 34, no. 22, article e149, 2006.
- S. Arnould, C. Perez, J. P. Cabaniols et al., “Engineered I-CreI derivatives cleaving sequences from the human XPC gene can induce highly efficient gene correction in mammalian cells,” Journal of Molecular Biology, vol. 371, no. 1, pp. 49–65, 2007.
- S. Arnould, C. Delenda, S. Grizot et al., “The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy,” Protein Engineering, Design and Selection, vol. 24, no. 1-2, pp. 27–31, 2011.
- P. Redondo, J. Prieto, I. G. Mũoz et al., “Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases,” Nature, vol. 456, no. 7218, pp. 107–111, 2008.
- K. H. Kraemer, M. M. Lee, and J. Scotto, “Xeroderma pigmentosum. Cutaneous, ocular, and neurologic abnormalities in 830 published cases,” Archives of Dermatology, vol. 123, no. 2, pp. 241–250, 1987.
- M. Fenina, D. Simon-Chazottes, S. Vandormael-Pournin et al., “I-SceI-mediated double-strand break does not increase the frequency of homologous recombination at the Dct Locus in mouse embryonic stem cells,” PLoS One, vol. 7, no. 6, Article ID e39895, 2012.
- N. Bennardo and J. M. Stark, “ATM limits incorrect end utilization during non- homologous end joining of multiple chromosome breaks,” PLoS Genetics, vol. 6, no. 11, Article ID e1001194, 2010.
- S. Grizot, J. C. Epinat, S. Thomas et al., “Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds,” Nucleic Acids Research, vol. 38, no. 6, Article ID gkp1171, pp. 2006–2018, 2009.