Study on Genetic Variance of miR-541 in Type 1 Diabetes
Figure 2
Restriction fragment length polymorphism (RFLP) analysis for -404 G→T. Polymerase chain reaction (PCR) amplification length was 992 bp. Nonmutated amplification region includes 1 DpnII site, generating 741 + 251 digested fragments. However, for heterozygote -404 G→T mutation, another DpnII site (GAGC→GATC) is present, thereby generating 4 digested fragments 741 + 680 + 251 + 61 (the 61 bp fragment was extremely small and hence could not be visualized). NC is the negative control (without enzymatic digestion), M1 and M2 for marker, *for samples with heterozygote -404 G→T mutation.
