The Escherichia coli GcvB sRNA Uses Genetic Redundancy to Control cycA Expression
Figure 5
(a) Effects of the allele plus additional mutations on cycA-lacZ expression. WT and ΔgcvB λcycA-lacZ lysogens transformed with the indicated plasmids were grown in LB (+Amp for transformants) to mid-log phase and assayed for β-galactosidase. (b) Northern analysis of GcvB. RNA was isolated from WT, ΔgcvB, or ΔgcvB transformed with the indicated plasmids and probed with either a DIG-labeled GcvB or 5S rRNA-specific DNA probe. Numbers above each lane indicate levels of GcvB relative to WT.
