Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages: Role in the Potentiation of Cellular Responses to LPS and LTA
Figure 6
LPS and LTA induce sustained generation of reactive oxygen species (ROS) in TPA- differentiated ML-1 cells. ML-1 cells were incubated with TPA 5 nM for three days followed by incubation with growth media in absence of TPA for three days. The differentiated ML-1 cells were harvested, washed with PBS, and ROS assessed using Luminol-derived luminescence. Extracellular hydrogen peroxide produced by 1 million cells was measured in Berthold luminometer in the presence or absence of 100 ng/mL LPS or 10 ng/mL. CL is quantified as cpm x 106 over the indicated time period. As indicated on the time curve, 10 μM DPI was injected directly into the reaction mixture. The luminol-dependent CL of undifferentiated ML-1 cells is at the background level of the Berthold luminometer. The figure depicts results from one of three replicate experiments.
