Review Article

Toll-Like Receptors as Novel Therapeutic Targets for Ovarian Cancer

Figure 4

Stimulation of A2780 ovarian cancer cells via TLR3. A270 human ovarian cancer cells were treated for 24 h with 10 μg/mL of poly(I:C) (Invivogen, San Diego, CA) admixed with lipofectamine (Invitrogen, Carlsbad, MA) following the manufacturer’s instructions. Then supernatants were recovered for analysis. Supernatants from two independent experiments were pooled, and the presence of different chemokines and cytokines in poly(I:C) treated (a) or controls (b) was analyzed by using the RayBio Human Cytokine Antibody Array 3 (Raybiotech Inc, Norcross, GA) following the manufacturer’s instructions. Finally, density values were analyzed by using the ImageJ program.
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