Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

Table 1

Comparison of detection limits of 3 serum proteins with the current HPLC-LIF system and available data from literature.

Sl. No.	Proteins	Literature Limit of Detection	Current HPLC-LIF Limit of Detection
1	Creatine Phosphokinase (CPK)	8 U/L^33	7.3 (0.05) fmoles
2	Ferritin	0.25 ng/mL^30 (≈52.1 picomoles)	76 (0.1) fmoles
3	Human Serum Albumin (HSA)	17.32 ng/mL^34 (≈0.26 nmoles)	11.6 (0.01) fmoles
4	Transferrin	50 pg/mL^31 (≈0.625 nmoles)	2.1 (0.001) fmoles
5	Insulin	2 ppm^28, 30 ppm^29	17.4 (0.01) fmoles