Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments
Figure 3
Carboxymethyl cellulase activity in cell-free culture supernatant (a) and cell debris (b) of isolated Bacillus subtilis strains. Bacterial cells (open rhombus, SL9-9; open square, C5-16; open triangle, S52-2; open circle, KACC10111) were grown in carboxymethyl cellulose (CMC) liquid medium (pH 7.0) on a shaker at 150 rpm for 7 days at 28°C. All cultures entered stationary phase in 5 days. Both cell-free culture supernatants (CFSs) and cell debris (CD) were assayed for CMCase activity. The mean values obtained from triplicate experiment were used to present results.
