Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments

Figure 6

Xylanase activity in cell-free culture supernatant of isolated Bacillus subtilis strains. Bacterial cells (open rhombus, SL9-9; open square, C5-16; open triangle, S52-2; open circle, KACC10111) were grown in carboxymethyl cellulose (CMC) liquid medium (pH 7.0) on a shaker at 150 rpm for 7 days at 28°C. All cultures entered stationary phase in 5 days. Both cell-free culture supernatants (CFSs) and cell debris (CD) were assayed for xylanase activity. No xylanase activity was detected in the CD fraction. The mean values obtained from triplicate experiment were used to present results.