Method Development and Validation of Darifenacin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry: Application to a Pharmacokinetic Study

Table 3

Stability of darifenacin in rat plasma samples.

	Bench-top stability		Autosampler stability		Long term stability		Freeze and thaw stability
	30 hr		65 hr		55 Days		Cycle 3 (48 hr)
Spiked plasma concentration; (pg/mL)	Concentration measured (𝑛=6); (pg/mL); (mean ± S.D)	CV (%) (𝑛=6)	Concentration measured (𝑛=6); (pg/mL); (mean ± S.D)	CV (%) (𝑛=6)	Concentration measured (𝑛=6); (pg/mL); (mean ± S.D)	CV (%) (𝑛=6)	Concentration measured (𝑛=6); (pg/mL); (mean ± S.D)	CV (%) (𝑛=6)
30.00	29.60±0.81	2.70	29.80±0.40	1.34	28.90±0.60	2.08	29.50±0.80	2.71
18000.00	17889.00±120.50	0.67	17923.00±118.50	0.66	17863.00±161.80	0.90	17943.00±135.20	0.75