Molecular Determinants of Ca<sub>v</sub>1.2 Calcium Channel Inactivation

Soldatov, Nikolai M.

doi:https://doi.org/10.5402/2012/691341

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Volume 2012 | Article ID 691341 | https://doi.org/10.5402/2012/691341

Molecular Determinants of Ca_v1.2 Calcium Channel Inactivation

Nikolai M. Soldatov¹

Academic Editor: E. Caffarelli, M. W. Berchtold, H.-C. Lee

Received24 Aug 2012

Accepted13 Sept 2012

Published17 Oct 2012

Abstract

Voltage-gated L-type Ca_v1.2 calcium channels couple membrane depolarization to transient increase in cytoplasmic free Ca²⁺ concentration that initiates a number of essential cellular functions including cardiac and vascular muscle contraction, gene expression, neuronal plasticity, and exocytosis. Inactivation or spontaneous termination of the calcium current through Ca_v1.2 is a critical step in regulation of these processes. The pathophysiological significance of this process is manifested in hypertension, heart failure, arrhythmia, and a number of other diseases where acceleration of the calcium current decay should present a benefit function. The central issue of this paper is the inactivation of the Ca_v1.2 calcium channel mediated by multiple determinants.

1. Introduction

The voltage-gated inward Ca²⁺ current () is a common mechanism of transient increase in the cytoplasmic free Ca²⁺ concentration triggered by cell depolarization. This form of Ca²⁺ signaling activates essential cellular processes including cardiac contraction [1], regulation of a smooth muscle tone [2], gene expression [3], synaptic plasticity [4] and exocytosis [5]. Complete and rapid termination of Ca²⁺ influx is mediated by an intricate mechanism of spontaneous calcium channel inactivation, which is crucial for preventing Ca²⁺ overloading of the cell during action potentials and restoration of the resting sub-μM cytoplasmic free Ca²⁺ concentration [6]. This paper will focus on the molecular basis and multiple determinants of the Ca_v1.2 calcium channel inactivation.

2. Ca_v1.2: Challenges and Solutions

2.1. Molecular Complexity

The Ca_v1.2 calcium channel is an oligomeric complex composed of the α_1C, α₂δ, and β subunits [7, 8]. The ion channel pore is formed by the α_1C peptide (Figure 1) that is encoded by the CACNA1C gene. The auxiliary β and α₂δ subunits are essential for the functional expression and plasma membrane (PM) targeting of the channel [9, 10]. They exist in multiple genomic isoforms generated by four CACNB genes (CACNB1–4) and three CACNA2D genes (CACNA2D1–3). All three subunits are subject to alternative splicing. Adding to the complexity of the Ca_v1.2 molecular organization, β subunits tend to oligomerize [11]. All together, genomic variability, alternative splicing, and hetero-oligomerization generate a plethora of Ca_v1.2 splice variants that are expressed in cells in species-, tissue-, and developmental-dependent manner, while the change of their fine balance may have significant pathophysiological consequences [12, 13].

Figure 1

Transmembrane topology of the α _1C subunit. To illustrate the sites of molecular diversity, the polypeptide sequence is schematically segmented according to the CACNA1C genomic map [14] and the corresponding invariant (black) and alternative (blue) exons are outlined by black bars and numbered (1–50). Four regions of homology (I–IV), each composed of 6 transmembrane segments (numbered), are believed to be folded around the central pore. α-interaction domain (AID) of a constitutive β-binding site is shown in green. LA and IQ motifs (red) constitute calmodulin-binding domain (CBD).

2.2. Challenges in the Selection of the Host Cell

Naturally occurring diversity of Ca_v1.2 complicates the interpretation of data obtained from native cells, let alone the single channel data. This underlies the importance of Ca_v1.2 research in recombinant expression systems where the molecular composition of the channel and the structure of its constituents are predefined. However, this experimental approach encountered the major problem of the selection of an appropriate host cell.

Most of the studies of calcium channels were carried out using HEK293 cells. These cells provide high expression efficiency of recombinant Ca²⁺ channels but, unfortunately, contain endogenous calcium channels exhibiting Ca²⁺ currents up to 3 pA/pF [15, 16]. Thus, HEK293 cells allow for the adequate study of recombinant Ca²⁺ channels only when the amplitude of the current is large enough to ignore the contribution of the endogenous channels. Correct assessment of the functional determinants of Ca²⁺ channels, however, requires the use of host cells that are completely free of endogenous Ca²⁺ channel subunits. COS1 or COS7 cells suit this requirement well because they generate no appreciable calcium current, do not contain endogenous Ca²⁺ channel subunits or their precursors, and show no induction of endogenous Ca_v1.2 subunits in response to the expression of the recombinant ones [17, 18]. Kinetics parameters and voltage dependence of activation and inactivation of the Ca_v1.2 channel currents measured in COS1 cells are consistent with data obtained in other expression systems [19]. An important advantage of COS cells is their relatively slow division rate that allows for better control over efficiency of expression and assembly of the Ca_v1.2 channel subunits of different size.

2.3. Problems of Fluorescent Labeling and Measurement

Fusion of GFP-like fluorophores to the N- and/or C-termini of the recombinant α_1C or to the N-terminus of β does not markedly change the electrophysiological properties of the expressed channels, enables the application of fluorescent and FRET (fluorescent resonance energy transfer) microscopy to the study of subcellular distribution and assembly of Ca_v1.2 as well as intricate aspects of molecular architecture and dynamics of the channel. The channel retains major electrophysiological characteristics unchanged when the α_1C C-terminal sequence encoded by distal exons 46–50 (Figure 1, residues 1833–2138 in α_1C,77) is replaced by ECFP. However, α_1C fused by its N- or/and C-termini to EYFP is highly sensitive to photobleaching that irreversibly inactivates it. Known as fluorophore-assisted light inactivation (FALI), this interesting property limits the applicability of acceptor photobleaching for the measurements of FRET in Ca_v1.2 because of uncertainty in the functional state of the channel [20]. However, the ratiometric analysis of corrected FRET between the fluorophores, fused to the tails of the α_1C and/or β subunits, reflects the reversible state-dependent structural rearrangements of the channel induced by the changes of transmembrane voltage under patch clamp [19, 21].

2.4. Recombinant Ca_v1.2: What Does It Need for Functional Expression and How Does It Appear?

Typical properties of a “wild-type” recombinant Ca_v1.2 are illustrated in Figure 2(A) using an example of the ubiquitous human α_1C,77 isoform (GenBank no. z34815). When the EYFP-labeled α_1C was expressed in COS1 cells alone, the fluorescent-tagged channel protein was diffusely distributed over the cytoplasm and did not generate measurable calcium current (Figure 2(A), panel a). The quantitative analysis of distribution of α_1C between PM and the cytoplasm (Figure 2(B)) [18] confirmed lack of significant PM targeting by α_1C independently on the presence of α₂δ (bars a and b). Expression of Ca_vβ in the absence of α₂δ stimulated PM targeting of α_1C, but the channel remained silent (Figure 2(A), panel c) unless α₂δ was coexpressed (panel d). Thus, β and α₂δ subunits are sufficient for the functional channel; under these experimental conditions, β subunits stimulate PM targeting of the channel complex and, in the presence of α₂δ, facilitate voltage gating of the Ca_v1.2 channel.

Figure 2

Role of the Ca_v1.2 auxiliary subunits. (A) Epifluorescent images of the expressing COS1 cells showing distribution of EYFP_N-α _1C obtained with the YFP filter (scaling bars, 4 μm) and traces of the maximum calcium current recorded in response to 600 ms steps to +30 mV from the holding potential mV (left). (B) Relative distribution of EYFP_N-α _1C in the plasma membrane (PM) over the cytoplasm in the absence (a) or presence of α ₂δ (b), β _2d (c), or α ₂δ + β _2d (d). The ratio of fluorescence intensity in PM over the area underneath PM was averaged after background subtraction in each cell. The ratio less than 1.0 indicates lack of significant PM targeting by α _1C. * [18].

The shape and appearance of the peak calcium current shown in Figure 2(A) (panel d) is quite typical for the β₂-modulated Ca_v1.2 [22]. Its major features include the relatively slow rate of decay and a large fraction of the sustained remaining the end of the depolarizing pulse [18]. It is clear that during long-lasting action potentials such properties may lead to pathogenic calcium overload of the cell if it is not balanced by robust compensatory mechanisms. It was the ultimate role of Ca_v1.2 in defining the duration of the action potential in cardiac cells that triggered the research and development of calcium channel blockers, a class of drugs that by now has a billion dollar market. It is this role of Ca_v1.2 that stimulates the current interest to the identification of molecular determinants of Ca_v1.2 inactivation in hopes of finding more specific and more effective drugs.

2.5. Last but Not Least a Complication: Ca_v1.2 Clustering

A single ventricular myocyte contains ~300,000 Ca_v1.2 channels, but only ~3% of the channels are open at peak [23]. Contrary to the popular belief, Ca_v1.2 channels are not evenly distributed over the plasma membrane. In native neuronal [24–26] and cardiac muscle cells [27–29] they form large clusters. Single-molecule imaging of the functional recombinant EYFP_N-α_1C/β_2a/α₂δ channels expressed in HEK293 cells revealed clusters composed of ~40 channels that were mobile in the plasma membrane [30]. Both the fluorescence correlation spectroscopy and fluorescence recovery after photobleaching experiments yielded a lateral diffusion constant of μm²/s. The functional significance of the Ca_v1.2 clusters mobility is not clear. It is believed that in cardiac muscle cells such mobility may be restrained by interactions with other proteins, for example, ryanodine receptors [27]. The size of Ca_v1.2 clusters and their specific density in the plasma membrane depend on the type of β subunit expressed [31]. The distance between the termini of neighbor α_1C subunits varies from 67 Å with neuronal/cardiac β_1b to 79 Å with vascular β₃. The highest density of Ca_v1.2 clusters in the plasma membrane and the smallest cluster size were observed with β_1b present. Insight into molecular mechanisms defining the architecture and properties of Ca_v1.2 clusters is important for better understanding of pathophysiology of the coupling between the Ca_v1.2 activity and the induced responses in Ca²⁺ signal transduction.

3. Voltage- and Ca²⁺-Dependent Inactivation of the Ca_v1.2 Calcium Channel

In the case of Ca_v1.2 calcium channels, two different mechanisms are in control of Ca²⁺ current inactivation. One mechanism is driven by Ca²⁺ ions on the cytoplasmic side of the plasma membrane, whereas the other depends on transmembrane voltage. Experimentally, replacement of Ca²⁺ for Ba²⁺ as the charge carrier eliminates Ca²⁺-dependent inactivation (CDI) [32] so that the Ba²⁺-conducting calcium channels inactivate in a voltage-dependent manner by fast (FI) and slow (SI) mechanisms [33]. These three mechanisms of inactivation, FI, SI and CDI, and their major determinants are illustrated on Figure 3.

Figure 3

Molecular determinants of Ca_v1.2 inactivation. Comparison of the wild-type Ca_v1.2 (A) with the same channel deprived of CDI (B) and SI (C) determinants. The five horizontal panels show (a) arrangement of critical determinants of inactivation. ADSI is composed of conserved hydrophobic amino acids in a -2 position of S6 segments in repeats II, III, and IV (yellow circles: Ala, Val, and Ile, resp.) as well as Ser residue in -1 position of IS6 (cyan circle). The CaM-binding domain (CBD) of the α _1C C-tail is shown by a red rounded rectangle. A β subunit (green) binds to the α-interaction domain in the linker between repeats I and II, and, in a Ca²⁺-dependent manner, to the IQ-region of the α _1C subunit C-tail ([45], not shown). The distal structure of β ₂ (β ₂CED, blue ball) binds to the CBD [46]. (b) Evidence of coimmunoprecipitation of the indicated subunits. (c) Normalized traces of (black) and (red), and (d) voltage dependence of (red) and time constant of FI (, black) are presented to illustrate CDI in (A) and lack of CDI in (B) and (C). (e) Link between CDI and differential β-subunit modulation (DβM) of Ca_v1.2. (A) Differential modulation of the inactivation by β _1a (black trace) and β _2a (green trace) in the WT Ca_v1.2. Disruption of CBD (α _1C,86) eliminates CDI and SI targeted by CDI and DβM (B). Mutation of ADSI (α _1C,IS-IV) removed CDI and fully inhibited SI so that the channel remains conducting for the duration of the depolarization stimulus (C).

3.1. Ca²⁺-Dependent Inactivation and Calmodulin-Binding Domain of α_1C

There are several different determinants of CDI, but it was not until 1997 that the Ca²⁺-sensing site of CDI had been narrowed down to a stretch of the 80-amino-acid C-terminal sequence of α_1C encoded by exons 40–42 [34] (Figure 2) marked by red block in Figure 3(A) (panel a). A naturally occurring splice variation in this region in α_1C,86 (Figure 3(B)) completely inhibited CDI as it is evident from the lack of deceleration of the current with Ba²⁺ as the charge carrier (panel c, black trace) as compared with (red trace). Another characteristic feature of the inhibited CDI was lack of the current size dependence of on voltage (Figure 3(B), panel d, open symbols) that stays in contrast to the U-shape dependence of the time constant of fast inactivation () on membrane potential in the wild-type Ca_v1.2 (see Figure 3(A), panel d). Two distinct sequences, L and K, were identified within this 80-amino-acid stretch whose α_1C,86-like mutations in the wild-type α_1C conform to the same characteristic features [35], suggesting the existence of two adjacent CDI sensors. One of them was outlined in the K region as the calmodulin- (CaM-) binding IQ motif [36] and, later on, the link of the IQ motif to CDI as the functional Ca²⁺-CaM binding site was confirmed in three independent studies [37–39] by the use of CaM mutants lacking affinity to Ca²⁺. Correspondingly, the LA motif was linked to CDI as apo-CaM binding site [40–42] endowed by the resting state of the channel. A single CaM molecule tethered to this Ca²⁺-dependent CaM-binding domain (CBD) of α_1C is the major Ca²⁺ sensor of the channel [43, 44].

Splice variation of α_1C in CBD region of α_1C,86 not only completely inhibits CDI, but also removes SI (Figure 3(B), panel c) and deprives the channel of differential sensitivity to β-subunit modulation (Figure 3(B), panel e) in spite of the fact that β remains associated with α_1C,86 (Figure 3(B), panel b). This indicates that all three properties of the channel—CDI, SI, and β-subunit modulation—are linked together [48, 49].

3.2. Slow Inactivation

A number of evidences have been presented that amino acids confined to the distal part of S6 segments in α_1C play important role in SI [50–52]. Systematic study of this region [53] outlined the “annual determinant of slow inactivation” (ADSI) as a structure composed of four highly conserved amino acids of four transmembrane segments S6, constituting the cytoplasmic end of the pore (Figure 3(A), panel a). Their simultaneous mutation (S405I in IS6, A752T in IIS6, V1165T in IIIS6, and I1475T in IVS6) generates the α_1C,IS-IV channel. Analysis of the current kinetics of the α_1C,IS-IV channel showed tremendous acceleration of the rapidly inactivating component ( ≤ 10 ms) that comprises about 50% of the total (or ) amplitude. Slow voltage-dependent inactivation of α_1C,IS-IV is fully inhibited, and the channel remains conducting for the duration of depolarization. Replacement of Ca²⁺ for Ba²⁺ as the charge carrier (panel c) did not change significantly this pattern of inactivation, while the analysis of voltage dependence of for the inactivating component of through the α_1C,IS-IV channel (panel d) confirmed lack of CDI. The replacement of β_1a for β_2a (panel e) did not change inactivation of the α_1C,IS-IV channel current suggesting lack of differential β-subunit modulation, while the co-immunoprecipitation analysis (panel b) provided direct evidence of association between α_1C,IS-IV and β.

Taken together, results presented in Figure 3 suggest that there is a cross-talk between ADSI, CBD and β, supported by direct interactions between them and/or specific conformational folding of the constituents of the polypeptide bundle underlying the pore. Indeed, both the interaction of β with CBD and the importance of functional conformation were directly demonstrated in live cells expressing recombinant Ca_v1.2.

3.3. Role of the α_1C C-tail Folding

Quantitative voltage-dependent FRET microscopy combined with patch clamp in the live cell showed that the α_1C subunit C-terminal tail is subject to reversible voltage-gated conformational rearrangements [21, 47]. The anchoring of the α_1C C-tail to the inner leaflet of the plasma membrane via the pleckstrin homology (PH) domain fused to the C-terminus of α_1C (α_1C-) abolished this conformational rearrangement and inhibited both SI and CDI (Figure 4(A)) in a manner very similar to that observed with α_1C,IS-IV (Figure 3(C)). This modification limiting the mobility of the α_1C carboxyl terminus had major implication on Ca²⁺ signal transduction. CREB-dependent transcriptional activation associated with the activity of Ca_v1.2 was completely suppressed in spite of robust generated by the “C-anchored” channel in response to depolarization. Release of the α_1C C-tail by activation of PIP₂ hydrolysis upon activation of phospholipase C fully restores all these deficient functions, including SI, CDI, and the effective coupling of to the CREB-dependent transcription [21]. Thus, it is specific functional folding of the α_1C C-terminal tail that is crucial for inactivation. It is crucial for signal transduction because it is designed to cage the permeating Ca²⁺ in CaM attached to CBD and to effectively move this caged Ca²⁺ to downstream signaling targets associated with CREB-dependent transcription or cardiac muscle contraction [54]. Above all, this function occurs in tight coordination with extracellular stimuli activating the channel. In terms of signal transduction, SI is a lock on the inside of the channel that is released by the permeating Ca²⁺ to accelerate its closure and initiate the movement of the C-terminal tail [49].

3.4. Role of the α_1C N-Terminus

All the functions mentioned above depend also on the integrity of the α_1C N-terminus. Inactivation properties of the recombinant α_1C/β/α₂δ channel are not greatly altered by structural changes of the proximal part of the α_1C N-tail, for example, by the fusion of a fluorescent protein [19, 21], by PH domain [47], or by alternative splicing of exons 1/1A generating the long isoform of α_1C [55]. The very first functional analysis of the effect of partial deletion of the α_1C N-terminus showed [56] that it is involved in inactivation while β prevents inhibition of the channel by the N-tail. Using FRET microscopy combined with patch clamp, we found that inactivation causes strong mutual reorientation of the α_1C and β_1a NH₂-termini, but their distance vis-à-vis the plasma membrane is not appreciably changed [19]. This relative lack of mobility is conferred by β in a manner that facilitates the channel response to voltage gating. Experiments on uncoupling of the α_1C subunit N-terminal tail from the regulation of the channel were carried out in the absence of β. Anchoring of the α_1C N-tail in the inner leaflet of the plasma membrane via attached PH domain created conditions when PH_N-α_1C and α₂δ were sufficient to generate a robust inward current (Figure 4(B)). This channel, however, is deprived of CDI and any voltage-dependent inactivation. Indeed, neither Ba²⁺ nor Ca²⁺ current has shown appreciable decay (see overlapped traces). Release of the α_1C N-tail upon PIP₂ hydrolysis by activation of phospholipase C completely inhibited the β-deficient channel [47]. Similar properties, except a much slower activation of the current, were observed on deletion of the entire (but 4 amino acids) N-terminal tail of α_1C (Figure 4(C)). With either type of uncoupling of the α_1C N-terminal tail—whether through a deletion or by PM anchoring,—a delay in the activation of the whole-cell current appears to be associated with prolongation of the first latency. Single channel recordings revealed that deletion of the N-tail essentially stabilized the open state of the Δ_N-α_1C/α₂δ channel, which showed longer openings during long-lasting depolarization [47].

Thus, CDI is mediated by CBD determinants of the α_1C C-tail, by the ADSI in the cytoplasmic pore region, and by the folding of the α_1C C- and N-termini. Calmodulin integrates these determinants, providing a Ca²⁺-dependent switch that terminates slow inactivation, releases the α_1C C-tail, and shuttles the associated Ca²⁺/calmodulin acting as an activating stimulus of the Ca²⁺ signal transduction [49].

3.5. Expression and Inactivation of Ca_v1.2 in the Absence of the β and α₂δ

Are the β and α₂δ subunits essential for the functional expression of the Ca_v1.2 channel? The analysis of the effects of exogenous CaM (CaM_ex) on the expression and properties of Ca_v1.2 in the absence of either β [18] or α₂δ [57] clearly demonstrated that neither β nor α₂δ is essential. Overexpression of CaM_ex only slightly modifies the voltage gating of the α_1C/β_2d/α₂δ channel by shifting the voltage dependence of activation and inactivation towards more negative potentials, facilitating (but not accelerating) inactivation, and increasing the density of approximately 2-fold [18]. CDI is retained, as it is evident from the effect of the replacement of Ca²⁺ for Ba²⁺ as the charge carrier that significantly increased the time course of inactivation of the current (Figure 5(A)). New understanding of the roles of β and α₂δ comes with the finding that CaM_ex renders expression and activity of the α_1C channel in the absence of β (Figure 5(B)) or α₂δ (Figure 5(C)), but not both of these auxiliary subunits. Although CaM_ex is structurally unrelated to β and α₂δ, it supports trafficking, CDI, and channel gating. Quantitative analysis showed that CaM_ex did not stimulate redistribution of α_1C in PM over the cytoplasm, but significantly enhanced plasma membrane targeting of α_1C/α₂δ channels. On the other hand, CaM_ex did not enhance the relative distribution of the α_1C/β_2d and α_1C/β_2d/α₂δ channels in the plasma membrane over the cytoplasm. Thus, depending on the auxiliary subunit present, CaM_ex-supported channel activity of α_1C/β_2d and α_1C/α₂δ is under control of different mechanisms. In spite of that, the CaM_ex-facilitated, single-auxiliary-subunit channels exhibit quite similar properties including significantly slower inactivation kinetics of the calcium current and a strong shift of the voltage dependence of activation and inactivation towards more negative potentials. Similar to the conventional α_1C/β_2d/α₂δ channels, these channels retain CDI and high sensitivity to dihydropyridine calcium channel blockers [18]. However, only the α_1C/β/CaM_ex channel shows facilitation of the calcium current by strong depolarization prepulse [57] (data not shown).

Because CaM associated with CBD is involved in CDI, it is clear that the effect of CaM_ex is mediated by different CaM-binding site(s). One of the potential candidates of such a site is present in the distal part of the α_1C N-terminal tail [58, 59]. It remains to be seen whether this site indeed plays an integrating role in the regulatory bundle of several molecular determinants supporting Ca_v1.2 inactivation. Another possibility confines the role of CaM_ex to the activation of silent Ca_v1.2 within the large clusters, where limited local availability of CaM may be the reason of the low fractional activity described in Section 2.5. Whatever the mechanisms associated with regulation of Ca_v1.2 by CaM are, they seems to have little practical implication for use in medicine at this time exactly because CaM is a ubiquitous and multifunctional peptide that regulates many other cellular functions, while its presence in Ca_v1.2 is vital for CDI.

4. β-Subunit Modulation of Ca_v1.2

Remarkable molecular variability of β subunits, reflected in altered inactivation properties of the differentially modulated Ca_v1.2 [12, 60], exemplified in Figure 3(A) (panel e) presents a new opportunity for the development of innovative approaches to the treatment of the diseases associated with Ca²⁺ mishandling. Several recent observations provide a foundation for such an optimistic view. First, β subunits exhibit a tendency to form homo- and hetero-oligomers [11, 61] that was directly demonstrated by a variety of biochemical techniques in both native cells and in recombinant expression system. While an augmentation of β homooligomerization significantly increases the density of , heterooligomerization of β₂ splice variants with other β subunits may also change the voltage-dependence and inactivation kinetics of Ca_v1.2 [11]. The β-oligomerization is mediated by several molecular determinants and thus needs multiple interventions to be managed, for example, in case of pathogenic overexpression of β₂. However, it seems to be more feasible to target β₂ itself; molecular determinant of β₂-specific slow and incomplete inactivation (see Figure 2(A), panel d) was identified [46] as the 40-amino-acid C-terminal determinant (β₂CED) present in all 7 known naturally occurring β₂ splice variants. Uncoupling of its Ca²⁺- and CaM-independent interaction with CBD (Figure 3(A), panel a) recovers the inactivation properties characteristic for β_1b/β₃-modulated Ca_v1.2 exhibiting rapid and complete inactivation of , as it was shown in deletion experiments. In my view, such selective uncoupling of β₂CED from binding to its receptor in CBD is a new attractive strategy to manage Ca²⁺ overload because other β subunits are not to be affected. Moreover, a cross-talk between Ca_v1.2 and the nearest target Ca²⁺/CaM-dependent protein kinase II [62, 63] will be preserved.

5. Conclusions

This paper has demonstrated that we know how to accelerate inactivation of Ca_v1.2 to less than 10 ms (Figure 3(C)), to deprive it from inactivation completely (Figures 4(B) and 4(C)), or to eliminate dependence of its expression from β or α₂δ without significant consequences for inactivation. We outlined the ultimate roles of the α_1C termini and CaM for inactivation, and yet none of these studies have brought us any closer to the ultimate goal of managing calcium mishandling associated with Ca_v1.2 except of old and, unfortunately, not too selective calcium channel blockers. The only new feasible target is pathogenic β₂ modulation of Ca_v1.2, where effector-receptor interaction is established.

In terms of molecular biology, Ca_v1.2 is certainly among the most complicated regulatory systems known. Remarkable molecular diversity of each of the Ca_v1.2 constituents gives rise to multiple genetic/splice variants of the channel that are subject to segregation into large and diverse clusters and to continuous functional change through homo- and hetero-oligomerization of β and other signaling components, not to speak about species, tissue, and developmental variability. We are surprised by the redundancy of the properties of multiple Ca_v1.2 isoforms [64, 65] and are even more surprised when some of them, showing just “conventional” electrophysiological properties, turn out to be associated with a disease [13]. In looking for an explanation, our insight should not be intuitively focused just on the characteristics of the calcium current-voltage dependence, amplitude, and duration. The end response, such as spatial and temporal organization of CREB signaling events associated with specific Ca_v1.2 isoform [66], and its competition with other (e.g., cAMP dependent) signaling mechanisms, or other Ca_v1.2 isoforms present, may provide new ideas and open new frontiers for investigation of the roles of individual Ca_v1.2 splice variants in normal and diseased cells and tissues.

Abbreviations

ADSI:	Annual determinant of slow inactivation
CaM:	Calmodulin
CBD:	Calmodulin-binding domain
CDI:	Ca-dependent inactivation
ECFP:	Enhanced cyan fluorescent protein
EYFP:	Enhanced yellow fluorescent protein
FALI:	Fluorophore-assisted light inactivation
FI:	Fast inactivation
FRET:	Fluorescent resonance energy transfer
GFP:	Green fluorescent protein
SI:	Slow voltage-dependent inactivation.

References

D. M. Bers, “Calcium cycling and signaling in cardiac myocytes,” Annual Review of Physiology, vol. 70, no. 1, pp. 23–49, 2008.
View at: Publisher Site | Google Scholar
M. T. Nelson, N. B. Standen, J. E. Brayden, and J. F. Worley, “Noradrenaline contracts arteries by activating voltage-dependent calcium channels,” Nature, vol. 336, no. 6197, pp. 382–385, 1988.
View at: Publisher Site | Google Scholar
H. Bading, D. D. Ginty, and M. E. Greenberg, “Regulation of gene expression in hippocampal neurons by distinct calcium signaling pathways,” Science, vol. 260, no. 5105, pp. 181–186, 1993.
View at: Google Scholar
D. Johnston, S. Williams, D. Jaffe, and R. Gray, “NMDA-receptor-independent long-term potentiation,” Annual Review of Physiology, vol. 54, pp. 489–505, 1992.
View at: Google Scholar
C. R. Artalejo, M. E. Adams, and A. P. Fox, “Three types of Ca²⁺ channel trigger secretion with different efficacies in chromaffin cells,” Nature, vol. 367, no. 6458, pp. 72–76, 1994.
View at: Publisher Site | Google Scholar
E. Carafoli, “Intracellular calcium homeostasis.,” Annual Review of Biochemistry, vol. 56, pp. 395–433, 1987.
View at: Google Scholar
V. Flockerzi, H. J. Oeken, and F. Hofmann, “Purification of a functional receptor for calcium-channel blockers from rabbit skeletal-muscle microsomes,” European Journal of Biochemistry, vol. 161, no. 1, pp. 217–224, 1986.
View at: Google Scholar
M. Takahashi, M. J. Seagar, J. F. Jones, B. F. X. Reber, and W. A. Catterall, “Subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle,” Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 15, pp. 5478–5482, 1987.
View at: Google Scholar
A. C. Dolphin, “β Subunits of voltage-gated calcium channels,” Journal of Bioenergetics and Biomembranes, vol. 35, no. 6, pp. 599–620, 2003.
View at: Publisher Site | Google Scholar
N. Klugbauer, E. Marais, and F. Hofmann, “Calcium channel α₂δ subunits: differential expression, function, and drug binding,” Journal of Bioenergetics and Biomembranes, vol. 35, no. 6, pp. 639–647, 2003.
View at: Publisher Site | Google Scholar
Q. Z. Lao, E. Kobrinsky, Z. Liu, and N. M. Soldatov, “Oligomerization of ${Ca}_{v} β$ subunits is an essential correlate of Ca²⁺ channel activity,” FASEB Journal, vol. 24, no. 12, pp. 5013–5023, 2010.
View at: Publisher Site | Google Scholar
H. M. Colecraft, B. Alseikhan, S. X. Takahashi et al., “Novel functional properties of Ca²⁺ channel β subunits revealed by their expression in adult rat heart cells,” Journal of Physiology, vol. 541, no. 2, pp. 435–452, 2002.
View at: Publisher Site | Google Scholar
S. Tiwari, Y. Zhang, J. Heller, D. R. Abernethy, and N. M. Soldatov, “Artherosclerosis-related molecular alteration of the human ${Ca}_{v} 1 .2$ calcium channel $α_{1C}$ subunit,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 45, pp. 17024–17029, 2006.
View at: Publisher Site | Google Scholar
N. M. Soldatov, “Genomic structure of human L-type Ca²⁺ channel,” Genomics, vol. 22, no. 1, pp. 77–87, 1994.
View at: Publisher Site | Google Scholar
S. Berjukow, F. Döring, M. Froschmayr, M. Grabner, H. Glossmann, and S. Hering, “Endogenous calcium channels in human embryonic kidney (HEK293) cells,” British Journal of Pharmacology, vol. 118, no. 3, pp. 748–754, 1996.
View at: Google Scholar
M. Kurejová, B. Uhrík, Z. Sulová, B. Sedláková, O. Križanová, and L. Lacinová, “Changes in ultrastructure and endogenous ionic channels activity during culture of HEK 293 cell line,” European Journal of Pharmacology, vol. 567, no. 1-2, pp. 10–18, 2007.
View at: Publisher Site | Google Scholar
A. Meir, D. C. Bell, G. J. Stephens, K. M. Page, and A. C. Dolphin, “Calcium channel β subunit promotes voltage-dependent modulation of α1B by Gβγ,” Biophysical Journal, vol. 79, no. 2, pp. 731–746, 2000.
View at: Google Scholar
A. Ravindran, Q. Z. Lao, J. B. Harry, P. Abrahimi, E. Kobrinsky, and N. M. Soldatov, “Calmodulin-dependent gating of ${Ca}_{v} 1 .2$ calcium channels in the absence of ${Ca}_{v} β$ subunits,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 23, pp. 8154–8159, 2008.
View at: Publisher Site | Google Scholar
E. Kobrinsky, K. J. F. Kepplinger, A. Yu et al., “Voltage-gated rearrangements associated with differential β-subunit modulation of the L-type Ca²⁺ channel inactivation,” Biophysical Journal, vol. 87, no. 2, pp. 844–857, 2004.
View at: Publisher Site | Google Scholar
E. Kobrinsky, J.-H. Lee, and N. M. Soldatov, “Selective fluorophore-assisted light inactivation of voltage-gated calcium channels,” Channels, vol. 6, no. 3, pp. 154–156, 2012.
View at: Publisher Site | Google Scholar
E. Kobrinsky, E. Schwartz, D. R. Abernethy, and N. M. Soldatov, “Voltage-gated mobility of the Ca²⁺ channel cytoplasmic tails and its regulatory role,” Journal of Biological Chemistry, vol. 278, no. 7, pp. 5021–5028, 2003.
View at: Publisher Site | Google Scholar
S. Herzig, I. F. Y. Khan, D. Gründemann et al., “Mechanism of ${Ca}_{v} 1 .2$ channel modulation by the amino terminus of cardiac β2-subunits,” FASEB Journal, vol. 21, no. 7, pp. 1527–1538, 2007.
View at: Publisher Site | Google Scholar
W. Y. W. Lew, L. V. Hryshko, and D. M. Bers, “Dihydropyridine receptors are primarily functional L-type calcium channels in rabbit ventricular myocytes,” Circulation Research, vol. 69, no. 4, pp. 1139–1145, 1991.
View at: Google Scholar
J. W. Hell, R. E. Westenbroek, C. Warner et al., “Identification and differential subcellular localization of the neuronal class C and class D L-type calcium channel α1 subunits,” Journal of Cell Biology, vol. 123, no. 4, pp. 949–962, 1993.
View at: Publisher Site | Google Scholar
D. Lipscombe, D. V. Madison, M. Poenie, H. Reuter, R. Y. Tsien, and R. W. Tsien, “Spatial distribution of calcium channels and cytosolic calcium transients in growth cones and cell bodies of sympathetic neurons,” Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 7, pp. 2398–2402, 1988.
View at: Publisher Site | Google Scholar
R. E. Westenbroek, M. K. Ahlijanian, and W. A. Catterall, “Clustering of L-type Ca²⁺ channels at the base of major dendrites in hippocampal pyramidal neurons,” Nature, vol. 347, no. 6290, pp. 281–284, 1990.
View at: Publisher Site | Google Scholar
C. Franzini-Armstrong, F. Protasi, and P. Tijskens, “The assembly of calcium release units in cardiac muscle,” Annals of the New York Academy of Sciences, vol. 1047, pp. 76–85, 2005.
View at: Publisher Site | Google Scholar
D. V. Gathercole, D. J. Colling, J. N. Skepper, Y. Takagishi, A. J. Levi, and N. J. Severs, “Immunogold-labeled L-type calcium channels are clustered in the surface plasma membrane overlying junctional sarcoplasmic reticulum in guinea-pig myocytes—implications for excitation-contraction coupling in cardiac muscle,” Journal of Molecular and Cellular Cardiology, vol. 32, no. 11, pp. 1981–1994, 2000.
View at: Publisher Site | Google Scholar
Y. Takagishi, K. Yasui, N. J. Severs, and Y. Murata, “Species-specific difference in distribution of voltage-gated L-type Ca²⁺ channels of cardiac myocytes,” American Journal of Physiology, vol. 279, no. 6, pp. C1963–C1969, 2000.
View at: Google Scholar
G. S. Harms, L. Cognet, P. H. M. Lommerse et al., “Single-molecule imaging of L-type Ca²⁺ channels in live cells,” Biophysical Journal, vol. 81, no. 5, pp. 2639–2646, 2001.
View at: Google Scholar
E. Kobrinsky, P. Abrahimi, S. Q. Duong et al., “Effect of ${Ca}_{v} β$ subunits on structural organization of ${Ca}_{v} 1 .2$ calcium channels,” PLoS ONE, vol. 4, no. 5, Article ID e5587, 2009.
View at: Publisher Site | Google Scholar
K. S. Lee, E. Marban, and R. W. Tsien, “Inactivation of calcium channels in mammalian heart cells: joint dependence on membrane potential and intracellular calcium,” Journal of Physiology, vol. 364, pp. 395–411, 1985.
View at: Google Scholar
X. Zong and F. Hofmann, “Ca²⁺-dependent inactivation of the class C L-type Ca²⁺ channel is a property of the α1 subunit,” FEBS Letters, vol. 378, no. 2, pp. 121–125, 1996.
View at: Publisher Site | Google Scholar
N. M. Soldatov, R. D. Zühlke, A. Bouron, and H. Reuter, “Molecular structures involved in L-type calcium channel inactivation. Role of the carboxyl-terminal region encoded by exons 40–42 in $α_{1C}$ subunit in the kinetics and Ca²⁺ dependence of inactivation,” Journal of Biological Chemistry, vol. 272, no. 6, pp. 3560–3566, 1997.
View at: Publisher Site | Google Scholar
N. M. Soldatov, M. Oz, K. A. O'Brien, D. R. Abernethy, and M. Morad, “Molecular determinants of L-type Ca²⁺ channel inactivation: segment exchange analysis of the carboxyl-terminal cytoplasmic motif encoded by exons 40-42 of the human $α_{1C}$ subunit gene,” Journal of Biological Chemistry, vol. 273, no. 2, pp. 957–963, 1998.
View at: Publisher Site | Google Scholar
R. D. Zühlke and H. Reuter, “Ca²⁺-sensitive inactivation of L-type Ca²⁺ channels depends on multiple cytoplasmic amino acid sequences of the $α_{1C}$ subunit,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 6, pp. 3287–3294, 1998.
View at: Google Scholar
B. Z. Peterson, C. D. DeMaria, J. P. Adelman, and D. T. Yue, “Calmodulin is the Ca²⁺ sensor for Ca²⁺-dependent inactivation of L-type calcium channels,” Neuron, vol. 22, no. 3, pp. 549–558, 1999.
View at: Google Scholar
N. Qin, R. Olcese, M. Bransby, T. Lin, and L. Birnbaumer, “Ca²⁺-induced inhibition of the cardiac Ca²⁺ channel depends on calmodulin,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 5, pp. 2435–2438, 1999.
View at: Google Scholar
R. D. Zühlke, G. S. Pitt, K. Deisseroth, R. W. Tsien, and H. Reuter, “Calmodulin supports both inactivation and facilitation of L-type calcium channels,” Nature, vol. 399, no. 6732, pp. 159–162, 1999.
View at: Publisher Site | Google Scholar
M. G. Erickson, B. A. Alseikhan, B. Z. Peterson, and D. T. Yue, “Preassociation of calmodulin with voltage-gated Ca²⁺ channels revealed by FRET in single living cells,” Neuron, vol. 31, no. 6, pp. 973–985, 2001.
View at: Publisher Site | Google Scholar
P. Pate, J. Mochca-Morales, Y. Wu et al., “Determinants for calmodulin binding on voltage-dependent Ca²⁺ channels,” Journal of Biological Chemistry, vol. 275, no. 50, pp. 39786–39792, 2000.
View at: Publisher Site | Google Scholar
C. Romanin, R. Gamsjaeger, H. Kahr et al., “Ca²⁺ sensors of L-type Ca²⁺ channel,” FEBS Letters, vol. 487, no. 2, pp. 301–306, 2000.
View at: Publisher Site | Google Scholar
M. X. Mori, M. G. Erickson, and D. T. Yue, “Functional stoichiometry and local enrichment of calmodulin interacting with Ca²⁺ channels,” Science, vol. 304, no. 5669, pp. 432–435, 2004.
View at: Publisher Site | Google Scholar
L. Xiong, Q. K. Kleerekoper, R. He, J. A. Putkey, and S. L. Hamilton, “Sites on calmodulin that interact with the C-terminal tail of ${Ca}_{v} 1 .2$ channel,” Journal of Biological Chemistry, vol. 280, no. 8, pp. 7070–7079, 2005.
View at: Publisher Site | Google Scholar
R. Zhang, I. Dzhura, C. E. Grueter, W. Thiel, R. J. Colbran, and M. E. Anderson, “A dynamic α-β inter-subunit agonist signaling complex is a novel feedback mechanism for regulating L-type Ca²⁺ channel opening,” FASEB Journal, vol. 19, no. 11, pp. 1573–1575, 2005.
View at: Publisher Site | Google Scholar
Q. Z. Lao, E. Kobrinsky, J. B. Harry, A. Ravindran, and N. M. Soldatov, “New determinant for the ${Ca}_{v} β$ ₂ subunit modulation of the ${Ca}_{v} 1 .2$ calcium channel,” Journal of Biological Chemistry, vol. 283, no. 23, pp. 15577–15588, 2008.
View at: Publisher Site | Google Scholar
E. Kobrinsky, S. Tiwari, V. A. Maltsev et al., “Differential role of the $α_{1C}$ subunit tails in regulation of the ${Ca}_{v} 1 .2$ channel by membrane potential, β subunits, and Ca²⁺ ions,” Journal of Biological Chemistry, vol. 280, no. 13, pp. 12474–12485, 2005.
View at: Publisher Site | Google Scholar
S. Hering, S. Berjukow, S. Aczél, and E. N. Timin, “Ca²⁺ channel block and inactivation: common molecular determinants,” Trends in Pharmacological Sciences, vol. 19, no. 11, pp. 439–443, 1998.
View at: Publisher Site | Google Scholar
N. M. Soldatov, “Ca²⁺ channel moving tail: link between Ca²⁺-induced inactivation and Ca²⁺ signal transduction,” Trends in Pharmacological Sciences, vol. 24, no. 4, pp. 167–171, 2003.
View at: Publisher Site | Google Scholar
S. Hering, S. Berjukow, S. Sokolov et al., “Molecular determinants of inactivation in voltage-gated Ca²⁺ channels,” Journal of Physiology, vol. 528, no. 2, pp. 237–249, 2000.
View at: Google Scholar
N. M. Soldatov, S. Zhenochin, B. AlBanna, D. R. Abernethy, and M. Morad, “New molecular determinant for inactivation of the human L-type $α_{1C}$ Ca²⁺ channel,” Journal of Membrane Biology, vol. 177, no. 2, pp. 129–135, 2000.
View at: Publisher Site | Google Scholar
J. F. Zhang, P. T. Ellinor, R. W. Aldrich, and R. W. Tsien, “Molecular determinants of voltage-dependent inactivation in calcium channels,” Nature, vol. 372, no. 6501, pp. 97–100, 1994.
View at: Publisher Site | Google Scholar
C. Shi and N. M. Soldatov, “Molecular determinants of voltage-dependent slow inactivation of the Ca²⁺ channel,” Journal of Biological Chemistry, vol. 277, no. 9, pp. 6813–6821, 2002.
View at: Publisher Site | Google Scholar
M. Morad and N. Soldatov, “Calcium channel inactivation: possible role in signal transduction and Ca²⁺ signaling,” Cell Calcium, vol. 38, no. 3-4, pp. 223–231, 2005.
View at: Publisher Site | Google Scholar
Y. Blumenstein, N. Kanevsky, G. Sahar, R. Barzilai, T. Ivanina, and N. Dascal, “A novel long N-terminal isoform of human L-type Ca²⁺ channel is up-regulated by protein kinase C,” Journal of Biological Chemistry, vol. 277, no. 5, pp. 3419–3423, 2002.
View at: Publisher Site | Google Scholar
E. Shistik, T. Ivanina, Y. Blumenstein, and N. Dascal, “Crucial role of N terminus in function of cardiac L-type Ca²⁺ channel and its modulation by protein kinase C,” Journal of Biological Chemistry, vol. 273, no. 28, pp. 17901–17909, 1998.
View at: Publisher Site | Google Scholar
A. Ravindran, E. Kobrinsky, Q. Z. Lao, and N. M. Soldatov, “Functional properties of the ${Ca}_{v} 1 .2$ calcium channel activated by calmodulin in the absence of α₂δ subunits,” Channels, vol. 3, no. 1, pp. 25–31, 2009.
View at: Google Scholar
A. Benmocha, L. Almagor, S. Oz, J. A. Hirsch, and N. Dascal, “Characterization of the calmodulin-binding site in the N terminus of ${Ca}_{v} 1 .2$ .,” Channels, vol. 3, no. 5, pp. 337–342, 2009.
View at: Google Scholar
T. Ivanina, Y. Blumenstein, E. Shistik, R. Barzilai, and N. Dascal, “Modulation of L-type Ca²⁺ channels by Gβγ and calmodulin via interactions with N and C termini of $α_{1C}$ ,” Journal of Biological Chemistry, vol. 275, no. 51, pp. 39846–39854, 2000.
View at: Publisher Site | Google Scholar
Z. Buraei and J. Yang, “The β subunit of voltage-gated Ca²⁺ channels,” Physiological Reviews, vol. 90, no. 4, pp. 1461–1506, 2010.
View at: Publisher Site | Google Scholar
E. Miranda-Laferte, G. Gonzalez-Gutierrez, S. Schmidt et al., “Homodimerization of the Src homology 3 domain of the calcium channel β-subunit drives dynamin-dependent endocytosis,” Journal of Biological Chemistry, vol. 286, no. 25, pp. 22203–22210, 2011.
View at: Publisher Site | Google Scholar
C. E. Grueter, S. A. Abiria, Y. Wu, M. E. Anderson, and R. J. Colbran, “Differential regulated interactions of calcium/calmodulin-dependent protein kinase II with isoforms of voltage-gated calcium channel β subunits,” Biochemistry, vol. 47, no. 6, pp. 1760–1767, 2008.
View at: Publisher Site | Google Scholar
O. M. Koval, X. Guan, Y. Wu et al., “ ${Ca}_{v} 1 .2$ β-subunit coordinates CaMKII-triggered cardiomyocyte death and afterdepolarizations,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 11, pp. 4996–5000, 2010.
View at: Publisher Site | Google Scholar
P. Liao, D. Yu, S. Lu et al., “Smooth muscle-selective alternatively spliced exon generates functional variation in ${Ca}_{v} 1 .2$ calcium channels,” Journal of Biological Chemistry, vol. 279, no. 48, pp. 50329–50335, 2004.
View at: Publisher Site | Google Scholar
P. Liao, D. Yu, G. Li et al., “A smooth muscle ${Ca}_{v} 1 .2$ calcium channel splice variant underlies hyperpolarized window current and enhanced state-dependent inhibition by nifedipine,” Journal of Biological Chemistry, vol. 282, no. 48, pp. 35133–35142, 2007.
View at: Publisher Site | Google Scholar
E. Kobrinsky, S. Q. Duong, A. Sheydina, and N. M. Soldatov, “Microdomain organization and frequency-dependence of CREB-dependent transcriptional signaling in heart cells,” FASEB Journal, vol. 25, no. 5, pp. 1544–1555, 2011.
View at: Publisher Site | Google Scholar

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Copyright © 2012 Nikolai M. Soldatov. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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International Scholarly Research Notices

Molecular Determinants of Cav1.2 Calcium Channel Inactivation

Abstract

1. Introduction

2. Cav1.2: Challenges and Solutions

2.1. Molecular Complexity

2.2. Challenges in the Selection of the Host Cell

2.3. Problems of Fluorescent Labeling and Measurement

2.4. Recombinant Cav1.2: What Does It Need for Functional Expression and How Does It Appear?

2.5. Last but Not Least a Complication: Cav1.2 Clustering

3. Voltage- and Ca2+-Dependent Inactivation of the Cav1.2 Calcium Channel

3.1. Ca2+-Dependent Inactivation and Calmodulin-Binding Domain of α1C