Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method

Table 2

Grading of ocular lesions by acute eye irritation test in rabbits.

Animal numbers	01				02				03				Mean	04				Mean
At hours	1	24	48	72	1	24	48	72	1	24	48	72		1	24	48	72
Reactions	Aqueous extract of Achyranthes aspera													Positive control (1% SDS)
Corneal opacity	0	0	0	0	0	0	0	0	0	0	0	0	0	2	3	2	2	2.33
Area of opacity	0	0	0	0	0	0	0	0	0	0	0	0	0	3	2	2	2	2.00
Iris	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Conjunctivae	1	1	0	0	1	1	1	0	1	1	1	0	0.55	0	0	0	0	0
Chemosis	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Note: grading of area of cornea involved.
Zero: 0.
One quarter (or less) but not zero: 1.
Greater than one quarter, but less than half: 2.
Greater than half, but less than three quarters: 3.
Greater than three quarters, up to whole area: 4.