Research Article
Antagonistic Roles for GcvA and GcvB in hdeAB Expression in Escherichia coli
Table 1
Strains, plasmids, and phage.
| Strain, plasmid, or phage | Relevant genotype | Source |
| Strain* | | | GS162 | WT | This lab | GS776 | cycA30::Tn10 | This lab | GS854 | argA81::Tn10 | This lab | GS998 | GS162 gcvA | [27, 28] | GS1132 | GS162 Δ(gcvAgcvB)::ΩaadA (referred to as ΔgcvAB) | [19] | GS1144 | GS162 ΔgcvB::ΩCMR (referred to as ΔgcvB) | [21] | GS1148 | GS162 hfq-1::ΩCMR (referred to as Δhfq) | [23] | Plasmid | | | pGS554 | Single-copy vector + constitutive gcvB (p) | [19] | pGS571 | Multicopy vector + WT gcvB (p) | [29] | pGS594 | Single-copy vector + WT gcvB (p) | This lab | pGS609 | Multi-copy vector + WT hfq (phfq 3+) | [23] | pGS611 | Multi-copy vector + WT gcvA (p) | This study | pGS624 | Multi-copy vector + WT gcvA gcvB (p) | This study | Phage | | | λgt2 | λ cloning vector; cI857 repressor | [30] | λhdeA::lacZ | λ vector carrying WT hdeA::lacZ fusion | This study | λhdeB::lacZ | λ vector carrying WT hdeB::lacZ fusion | This study | ::lacZ | λ vector carrying ::lacZ transcriptional fusion | This study | λPBAD::hdeA::lacZ | λ vector carrying hdeA::lacZ fusion under control of the PBAD promoter | This study |
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*All strains also carry the pheA905 thi araD129 rpsL150 relA1 deoC1 flbB5301 ptsF25 rbsR mutations.
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