Antagonistic Roles for GcvA and GcvB in hdeAB Expression in Escherichia coli

Table 1

Strains, plasmids, and phage.

Strain, plasmid, or phage	Relevant genotype	Source
Strain*
GS162	WT	This lab
GS776	cycA30::Tn10	This lab
GS854	argA81::Tn10	This lab
GS998	GS162 gcvA	[27, 28]
GS1132	GS162 Δ(gcvAgcvB)::ΩaadA (referred to as ΔgcvAB)	[19]
GS1144	GS162 ΔgcvB::ΩCM^R (referred to as ΔgcvB)	[21]
GS1148	GS162 hfq-1::ΩCM^R (referred to as Δhfq)	[23]
Plasmid
pGS554	Single-copy vector + constitutive gcvB (p𝑔𝑐𝑣𝐵^2+)	[19]
pGS571	Multicopy vector + WT gcvB (p𝑔𝑐𝑣𝐵^3+)	[29]
pGS594	Single-copy vector + WT gcvB (p𝑔𝑐𝑣𝐵^+)	This lab
pGS609	Multi-copy vector + WT hfq (phfq ^3+)	[23]
pGS611	Multi-copy vector + WT gcvA (p𝑔𝑐𝑣𝐴^3+)	This study
pGS624	Multi-copy vector + WT gcvA gcvB (p𝑔𝑐𝑣𝐴^3+𝑔𝑐𝑣𝐵^3+)	This study
Phage
λgt2	λ cloning vector; cI857 repressor	[30]
λhdeA::lacZ	λ vector carrying WT hdeA::lacZ fusion	This study
λhdeB::lacZ	λ vector carrying WT hdeB::lacZ fusion	This study
𝜆ℎ𝑑𝑒𝐴^−36::lacZ	λ vector carrying ℎ𝑑𝑒𝐴^−36::lacZ transcriptional fusion	This study
λPBAD::hdeA::lacZ	λ vector carrying hdeA::lacZ fusion under control of the PBAD promoter	This study

*All strains also carry the pheA905 thi araD129 rpsL150 relA1 deoC1 flbB5301 ptsF25 rbsR mutations.