Research Article

Antagonistic Roles for GcvA and GcvB in hdeAB Expression in Escherichia coli

Table 2

Effect of GcvA and GcvB on growth at low pH.

StrainGT (min) grown in LB at
pH 7.0pH 4.5*

(1) WT 5 2 ± 5 1 3 3 ± 2
(2) ΔgcvAB 5 6 ± 2 1 6 0 ± 6
(3) WT[p 𝑔 𝑐 𝑣 𝐴 3 + ] 6 7 ± 7 3 0 6 ± 9
(4) ΔgcvAB[p 𝑔 𝑐 𝑣 𝐴 3 + ] 6 0 ± 6 4 3 7 ± 4 5
(5) WT[p 𝑔 𝑐 𝑣 𝐴 3 + 𝑔 𝑐 𝑣 𝐵 3 + ] 6 7 ± 7 1 5 7 ± 4
(6) ΔgcvAB[p 𝑔 𝑐 𝑣 𝐴 3 + 𝑔 𝑐 𝑣 𝐵 3 + ] 6 2 ± 1 1 5 0 ± 5

*Cultures were tested at the end of the experiment to verify the pH had not changed.