Figure 1: A and B: Effects of WA on MDA-MB-231 (a) and BT20 (b) cell apoptosis as measured by Annexin V-FITC-propidium iodide flow cytometry. Cells were treated with indicated concentrations of WA for 24 h. Results were presented as mean () SD. *, , significantly different from control by one-way ANOVA. (C) Western analysis of the expression of PARP and caspase-3 in MDA-MB-231 and BT20 cells. Cells were treated with indicated concentrations of WA for 24 h. Western for -actin was conducted to confirm equal loading of proteins.