Cytokines and Growth Factors Promote Airway Smooth Muscle Cell Proliferation
Figure 6
Induction of the MAPK and PI3K signalling in rabbit tracheal ASMCs incubated with bFGF and PDGF. Left: western blot analysis with (a) antiphospho-p42/44, (b) antiphospho-p38, and (c) antiphospho-Akt and anti-β-actin antibodies in total protein cell extracts from rabbit tracheal smooth muscle cells incubated for 4 h with bFGF (10 ng/mL) or PDGF (25 ng/mL). Right: mean fold expression of phospho-p42/44, phospho-p38, or phospho-Akt normalized to β-actin. Data are presented as mean ± SEM, and refers to the number of independent experiments. *, **, and *** compared to control (one-way ANOVA with statistically significant differences between groups being determined by Bonferroni’s posttest).